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However, this approach faces several limitations, including low DNA-capture efficiency, limited molecular selectivity and the risk of contamination during filtration and handling. Moreover, commonly used membrane materials often lack strong and specific DNA-binding affinity, which reduces detection sensitivity and compromises biodiversity assessment. To address these challenges, we developed a MoS2-coated mixed cellulose ester membrane that significantly enhances both the efficiency and the selectivity in capturing eDNA. The MoS2 coating facilitates preferential interactions with DNA via van der Waals forces between the sulfur atoms in MoS2 and the oxygen or nitrogen atoms in DNA bases. Laboratory and field tests with marine fish confirmed that the MoS2-coated membrane significantly enhanced the selectivity and sensitivity of eDNA-based biota detection. This work represents the first application of a 2D nanomaterial-based membrane for eDNA collection and detection. Furthermore, the membrane is low-cost and scalable, and requires no further processing. This invention offers a promising strategy for the development of 2D nanomaterials-based eDNA-sampling tools and showcases their broader potential in environmental biotechnology applications.<\/jats:p>","DOI":"10.1093\/nsr\/nwag055","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:41:40Z","timestamp":1769604100000},"source":"Crossref","is-referenced-by-count":3,"title":["A tailored MoS2 membrane with strong DNA-binding capability enhances aquatic biota detection through environmental DNA metabarcoding"],"prefix":"10.1093","volume":"13","author":[{"given":"Liang","family":"Mei","sequence":"first","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]},{"name":"State Key Laboratory of Marine Environmental Health, City University of Hong Kong , Hong Kong 999077 ,","place":["China"]},{"name":"School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou 510641 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Ming","family":"How","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Health, City University of Hong Kong , Hong Kong 999077 ,","place":["China"]},{"name":"Hong Kong Baptist University Department of Biology, , Hong Kong 999077 ,","place":["China"]},{"name":"National Observation and Research Station of Coastal Ecological Environments in Macao, Macau Environmental Research Institute, Macau University of Science and Technology , Macau 999078 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzi","family":"Sun","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Chemistry, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixin","family":"Yan","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikang","family":"Zheng","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglu","family":"Hu","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2526-2002","authenticated-orcid":false,"given":"Bolong","family":"Huang","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Chemistry, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Wen","family":"Qiu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Health, City University of Hong Kong , Hong Kong 999077 ,","place":["China"]},{"name":"Hong Kong Baptist University Department of Biology, , Hong Kong 999077 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Zeng","sequence":"additional","affiliation":[{"name":"City University of Hong Kong Department of Materials Science and Engineering, , Hong Kong 999077 ,","place":["China"]},{"name":"State Key Laboratory of Marine Environmental Health, City University of Hong Kong , Hong Kong 999077 ,","place":["China"]},{"name":"Shenzhen Research Institute, City University of Hong Kong , Shenzhen 518057 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth M Y","family":"Leung","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Health, City University of Hong Kong , Hong Kong 999077 ,","place":["China"]},{"name":"City University of Hong Kong Department of Chemistry, , Hong Kong 999077 ,","place":["China"]},{"name":"Shenzhen Research Institute, City University of Hong Kong , Shenzhen 518057 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"2026031910261974700_bib1","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.ecss.2015.04.008","article-title":"Marine biodiversity and ecosystem function relationships: the 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