{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T01:49:36Z","timestamp":1783734576334,"version":"3.55.0"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T00:00:00Z","timestamp":1593648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Sant\u00e9 Publique France"},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Syndromic respiratory panels are increasingly used worldwide. Their performance for detection of Bordetella pertussis needs to be evaluated. We found that the FilmArray Respiratory Panel 2plus (RP2+) assay, which uses the pertussis toxin promoter target for B. pertussis, can only detect highly charged samples. Negative RP2+\u2005results should not be interpreted as an absence of B. pertussis in clinical samples.<\/jats:p>","DOI":"10.1093\/ofid\/ofaa267","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T17:54:04Z","timestamp":1593712444000},"source":"Crossref","is-referenced-by-count":11,"title":["Low Detection Rate of Bordetella pertussis Using the BioFire FilmArray Respiratory Panel 2plus"],"prefix":"10.1093","volume":"7","author":[{"given":"Sophie","family":"Guillot","sequence":"first","affiliation":[{"name":"Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France"},{"name":"National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assaf","family":"Mizrahi","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Saint Joseph Hospital, Paris, France"},{"name":"Institut Micalis, UMR1319, Universit\u00e9 Paris-Saclay, INRAe, AgroParisTech, Bact\u00e9ries Pathog\u00e8nes et Sant\u00e9, Chatenay-Malabry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Armatys","sequence":"additional","affiliation":[{"name":"Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France"},{"name":"National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laureen","family":"Chat","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Saint Joseph Hospital, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alban","family":"Le Monnier","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Saint Joseph Hospital, Paris, France"},{"name":"Institut Micalis, UMR1319, Universit\u00e9 Paris-Saclay, INRAe, AgroParisTech, Bact\u00e9ries Pathog\u00e8nes et Sant\u00e9, Chatenay-Malabry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Brisse","sequence":"additional","affiliation":[{"name":"Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France"},{"name":"National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9561-669X","authenticated-orcid":false,"given":"Julie","family":"Toubiana","sequence":"additional","affiliation":[{"name":"Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France"},{"name":"National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, Paris, France"},{"name":"General Paediatrics and Paediatric Infectious Diseases Department, H\u00f4pital Necker-Enfants Malades, APHP, Universit\u00e9 de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,2]]},"reference":[{"key":"2020080913462246500_CIT0001","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/5584_2019_408","article-title":"Pertussis: identification, prevention and control","volume":"1183","author":"Stefanelli","year":"2019","journal-title":"Adv Exp Med Biol"},{"key":"2020080913462246500_CIT0002","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/S0140-6736(06)68848-X","article-title":"Recent developments in pertussis","volume":"367","author":"Crowcroft","year":"2006","journal-title":"Lancet"},{"key":"2020080913462246500_CIT0003","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1097\/INF.0b013e3181f537c6","article-title":"Bordetella pertussis infection is common in nonvaccinated infants admitted for bronchiolitis","volume":"29","author":"Nuolivirta","year":"2010","journal-title":"Pediatr Infect Dis J"},{"key":"2020080913462246500_CIT0004","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1586\/14760584.2014.950237","article-title":"Pertussis diagnostics: overview and impact of immunization","volume":"13","author":"Wirsing von K\u00f6nig","year":"2014","journal-title":"Expert Rev Vaccines"},{"key":"2020080913462246500_CIT0005","doi-asserted-by":"crossref","first-page":"e00806-16","DOI":"10.1128\/JB.00806-16","article-title":"The history of Bordetella pertussis genome evolution includes structural rearrangement","volume":"199","author":"Weigand","year":"2017","journal-title":"J Bacteriol"},{"key":"2020080913462246500_CIT0006","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s10096-012-1718-3","article-title":"Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome","volume":"32","author":"Tizolova","year":"2013","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2020080913462246500_CIT0007","doi-asserted-by":"crossref","first-page":"4347","DOI":"10.1128\/JCM.01272-11","article-title":"Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis","volume":"49","author":"Njamkepo","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2020080913462246500_CIT0008","volume-title":"FilmArray Respiratory Panel 2 (RP2plus) Instruction Booklet RFIT-ASY-0136","author":"BioFire Diagnostics","year":"2017"},{"key":"2020080913462246500_CIT0009","article-title":"Multicenter evaluation of BioFire FilmArray respiratory panel 2 for detection of viruses and bacteria in nasopharyngeal swab samples","volume":"56:e01945-17","author":"Leber","year":"2018","journal-title":"J Clin Microbiol"},{"key":"2020080913462246500_CIT0010","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1128\/CMR.00031-15","article-title":"Laboratory diagnosis of pertussis","volume":"28","author":"van der Zee","year":"2015","journal-title":"Clin Microbiol Rev"},{"key":"2020080913462246500_CIT0011","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/j.1469-0691.2010.03255.x","article-title":"Marked difference between adults and children in Bordetella pertussis DNA load in nasopharyngeal swabs","volume":"17","author":"Nakamura","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"2020080913462246500_CIT0012","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1002\/jcla.21576","article-title":"Evaluation of the FilmArray\u00ae respiratory panel for clinical use in a large children\u2019s hospital","volume":"27","author":"Couturier","year":"2013","journal-title":"J Clin Lab Anal"},{"key":"2020080913462246500_CIT0013","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1128\/JCM.05996-11","article-title":"Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children","volume":"50","author":"Pierce","year":"2012","journal-title":"J Clin Microbiol"},{"key":"2020080913462246500_CIT0014","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1136\/jclinpath-2014-202833","article-title":"Testing implications of varying targets for Bordetella pertussis: comparison of the FilmArray respiratory panel and the focus B. pertussis PCR assay","volume":"68","author":"Jerris","year":"2015","journal-title":"J Clin Pathol"},{"key":"2020080913462246500_CIT0015","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1097\/INF.0b013e318197fac1","article-title":"Outbreak of atypical pertussis detected by polymerase chain reaction in immunized preschool-aged children","volume":"28","author":"Waters","year":"2009","journal-title":"Pediatr Infect Dis J"},{"key":"2020080913462246500_CIT0016","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.diagmicrobio.2008.02.017","article-title":"Development and evaluation of dual-target real-time polymerase chain reaction assays to detect Bordetella spp","volume":"61","author":"Tatti","year":"2008","journal-title":"Diagn Microbiol Infect Dis"}],"container-title":["Open Forum Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ofid\/advance-article-pdf\/doi\/10.1093\/ofid\/ofaa267\/33457831\/ofaa267.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ofid\/article-pdf\/7\/8\/ofaa267\/33620658\/ofaa267.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ofid\/article-pdf\/7\/8\/ofaa267\/33620658\/ofaa267.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T21:08:03Z","timestamp":1616447283000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ofid\/article\/doi\/10.1093\/ofid\/ofaa267\/5866603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,2]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8,1]]}},"URL":"https:\/\/doi.org\/10.1093\/ofid\/ofaa267","relation":{},"ISSN":["2328-8957"],"issn-type":[{"value":"2328-8957","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,8]]},"published":{"date-parts":[[2020,7,2]]},"article-number":"ofaa267"}}