{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:21Z","timestamp":1788568581536,"version":"build-2803163510"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Pulmonary infections due to Aspergillus, Mucorales, and Nocardia have high morbidity and mortality, in part due to diagnostic challenges. Commercially available molecular assays on bronchoalveolar lavage fluid (BALF) may have increased sensitivity over currently available diagnostic options. Our aim was to characterize the diagnostic performance of assays for each of these pathogens in our patient population.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The medical records of patients whose BALF was tested by polymerase chain reaction (PCR) for Aspergillus, Mucorales, and Nocardia between 2019 and 2021 were reviewed in a cross-sectional manner. European Organization for Research and Treatment of Cancer and the Mycoses Study Group (EORTC\/MSG) definitions of \u201cproven,\u201d \u201cprobable,\u201d and \u201cpossible\u201d infection were used, including histopathology, serology, and culture. We used (1) \u201cproven\u201d or \u201cprobable\u201d infection by EORTC criteria, (2) improvement or stabilization on targeted antimicrobial therapy, and (3) absence of a more likely diagnosis as the reference standard.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The Aspergillus PCR assay demonstrated the highest agreement with the diagnostic reference standard, with 31.25% (10\/32) sensitivity and 97.17% (206\/212) specificity. Positive and negative predictive values were 62.50% (10\/16) and 90.35% (206\/228), respectively. No Mucorales or Nocardia infections were identified by the diagnostic reference standard, so the sensitivity could not be calculated. The specificity of Mucorales and Nocardia targets was 98.35% and 96.69%, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our data demonstrated relatively poor clinical sensitivity for all 3 constituent PCR assays in our patient population, suggesting a limited role for this test in the diagnosis of Aspergillus, Mucorales, or Nocardia.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofac646","type":"journal-article","created":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T05:17:44Z","timestamp":1670303864000},"source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of the Diagnostic Accuracy and Clinical Utility of Fungal Profile Plus Polymerase Chain Reaction Assay in Pulmonary Infections"],"prefix":"10.1093","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1572-295X","authenticated-orcid":false,"given":"Clarissa B","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosong","family":"Shi","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, The University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael M","family":"Liesman","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A","family":"Thomas","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, The University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan C","family":"Bahr","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle R","family":"Brownback","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, The University of Kansas Medical Center , Kansas City, Kansas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"2022122708005091600_ofac646-B1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.3109\/13693786.2012.738312","article-title":"Global burden of allergic bronchopulmonary aspergillosis with asthma and its complication chronic pulmonary aspergillosis in 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