{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:50:01Z","timestamp":1785423001098,"version":"3.56.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T00:00:00Z","timestamp":1691625600000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Zhejiang Medical and Health Science and Technology","award":["2022KY373"],"award-info":[{"award-number":["2022KY373"]}]},{"name":"Key Construction Disciplines of Provincial and Municipal Co-construction of Zhejiang","award":["2023-SSGJ-002"],"award-info":[{"award-number":["2023-SSGJ-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Bronchiectasis is a chronic inflammatory respiratory disease mainly caused by pathogenic infections. However, standard methods of pathogen detection show prolonged cycle durations and unsatisfactory sensitivity and detection rates. Macrogenomic next-generation sequencing (mNGS) emerges as a promising technique for swift, effective, and unbiased pathogen detection and subsequent data interpretation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Here, a retrospective analysis of 93 patients with suspected bronchiectasis was performed to assess the clinical applicability of mNGS. Bronchoalveolar alveolar lavage fluid (BALF) samples were collected from these subjects, followed by standard assays and mNGS separately. The turnaround time, detection rate, and pathogen identification using mNGS were compared with those of standard methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>mNGS identified a greater number of bacteria (72 vs 16), fungi (26 vs 19), and viruses (14 vs 0) than standard methods. Specifically, the commonly identified bacteria were Haemophilus, Mycobacterium intracellulare, Pseudomonas, and Streptococcus pneumoniae, while the most detected fungi were Aspergillus and the most prevalent viruses were human herpesviruses. Of note, 29 out of 30 patients (96.67%) who received optimized treatment strategies based on mNGS results experienced recovery.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Collectively, these findings suggest that mNGS has the potential to improve the diagnosis and treatment of bronchiectasis patients by enabling rapid and precise pathogen detection, which can lead to timely and effective treatment strategies.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofad425","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T20:47:36Z","timestamp":1691700456000},"source":"Crossref","is-referenced-by-count":15,"title":["The Utility of Metagenomic Next-Generation Sequencing (mNGS) in the Management of Patients With Bronchiectasis: A Single-Center Retrospective Study of 93 Cases"],"prefix":"10.1093","volume":"10","author":[{"given":"Hui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfeng","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyue","family":"Yang","sequence":"additional","affiliation":[{"name":"Biological Medicine Research and Development Center, Yangtze Delta of Zhejiang , Hangzhou, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linfeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmin","family":"Wang","sequence":"additional","affiliation":[{"name":"Biological Medicine Research and Development Center, Yangtze Delta of Zhejiang , Hangzhou, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9516-9523","authenticated-orcid":false,"given":"Xiaolong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University), The Key Laboratory of Precision Therapy for Lung Cancer , Jiaxing, Zhejiang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,8,10]]},"reference":[{"key":"2023083123452947700_ofad425-B1","doi-asserted-by":"crossref","first-page":"105940","DOI":"10.1016\/j.rmed.2020.105940","article-title":"Non-CF bronchiectasis: orphan disease no longer","volume":"166","author":"Imam","year":"2020","journal-title":"Respir Med"},{"key":"2023083123452947700_ofad425-B2","doi-asserted-by":"crossref","first-page":"190055","DOI":"10.1183\/16000617.0055-2019","article-title":"The biology of pulmonary exacerbations in bronchiectasis","volume":"28","author":"Amati","year":"2019","journal-title":"Eur Respir Rev"},{"key":"2023083123452947700_ofad425-B3","first-page":"Cd012579","article-title":"Oral versus inhaled antibiotics for bronchiectasis","volume":"3","author":"Spencer","year":"2018","journal-title":"Cochrane Database Syst 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the aetiology, epidemiology and microbiology of bronchiectasis","volume":"18","author":"Chandrasekaran","year":"2018","journal-title":"BMC Pulm Med"},{"key":"2023083123452947700_ofad425-B8","doi-asserted-by":"crossref","first-page":"776","DOI":"10.5858\/arpa.2016-0539-RA","article-title":"Validation of metagenomic next-generation sequencing tests for universal pathogen detection","volume":"141","author":"Schlaberg","year":"2017","journal-title":"Arch Pathol Lab Med"},{"key":"2023083123452947700_ofad425-B9","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s12879-020-05746-5","article-title":"The diagnostic value of metagenomic next-generation sequencing in infectious diseases","volume":"21","author":"Duan","year":"2021","journal-title":"BMC Infect Dis"},{"key":"2023083123452947700_ofad425-B10","doi-asserted-by":"crossref","first-page":"671109","DOI":"10.3389\/fcimb.2021.671109","article-title":"Combining metagenomic sequencing with whole exome sequencing to optimize clinical strategies in neonates with a suspected central nervous system infection","volume":"11","author":"Ge","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"2023083123452947700_ofad425-B11","doi-asserted-by":"crossref","first-page":"875822","DOI":"10.3389\/fcimb.2022.875822","article-title":"The effectiveness of metagenomic next-generation sequencing in the diagnosis of prosthetic joint infection: a systematic review and meta-analysis","volume":"12","author":"Tan","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"2023083123452947700_ofad425-B12","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1080\/1040841X.2019.1681933","article-title":"mNGS in clinical microbiology laboratories: on the road to maturity","volume":"45","author":"Han","year":"2019","journal-title":"Crit Rev Microbiol"},{"key":"2023083123452947700_ofad425-B13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13073-016-0326-8","article-title":"Next-generation sequencing diagnostics of bacteremia in septic patients","volume":"8","author":"Grumaz","year":"2016","journal-title":"Genome Med"},{"key":"2023083123452947700_ofad425-B14","doi-asserted-by":"crossref","first-page":"309","DOI":"10.3390\/cancers11030309","article-title":"Compositional and functional differences between microbiota and cervical carcinogenesis as identified by shotgun metagenomic sequencing","volume":"11","author":"Kwon","year":"2019","journal-title":"Cancers (Basel)"},{"key":"2023083123452947700_ofad425-B15","doi-asserted-by":"crossref","first-page":"4924","DOI":"10.21037\/jtd-20-930","article-title":"Comparison of the next-generation sequencing (NGS) technology with culture methods in the diagnosis of bacterial and fungal infections","volume":"12","author":"Chen","year":"2020","journal-title":"J Thorac 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