{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:42:18Z","timestamp":1790185338234,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japanese Association of Infectious Diseases"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The genus Aeromonas is increasingly implicated in human infections, but knowledge of its clinical characteristics and antimicrobial resistance profiles has been limited owing to its complex taxonomy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a multicenter prospective cohort study of patients with Aeromonas infections at hospitals across Japan. Patients were eligible for inclusion if they had an Aeromonas spp. strain in a clinical culture and were considered infected at the culture site. Clinical data were collected, and isolates underwent susceptibility testing and whole-genome sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 144 patients were included. Hepatobiliary infection accounted for a majority of infections (73% [105 of 144]), which mostly occurred in elderly patients with comorbid conditions, including hepatobiliary complications. The all-cause 30-day mortality rate was 10.0% (95% confidence interval, 4.9%\u201314.8%). By whole-genome sequencing, 141 strains (98%) belonged to 4 Aeromonas species\u2014A caviae, A hydrophila, A veronii, and A dhakensis\u2014with significant intraspecies diversity. A caviae was predominant in all infection sites except skin and soft tissue, for which A hydrophila was the prevailing species. The genes encoding chromosomally mediated class B, C, and D \u03b2-lactamases were harbored by 92%\u2013100% of the isolates in a species-specific manner, but they often lacked association with resistance phenotypes. The activity of cefepime was reliable. All isolates of A hydrophila and A dhakensis carried an mcr-3-like colistin resistance gene and showed reduced susceptibility to colistin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Hepatobiliary tract was the most common infection site of Aeromonas spp., with A caviae being the dominant causative species. The resistance genotype and phenotype were often incongruent for \u03b2-lactam agents.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofad587","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T11:23:48Z","timestamp":1700047428000},"source":"Crossref","is-referenced-by-count":32,"title":["Clinical Features, Genome Epidemiology, and Antimicrobial Resistance Profiles of\n                    <i>Aeromonas<\/i>\n                    spp. Causing Human Infections: A Multicenter Prospective Cohort Study"],"prefix":"10.1093","volume":"10","author":[{"given":"Aki","family":"Sakurai","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Fujita Health University School of Medicine , Aichi , Japan"},{"name":"Department of Microbiology, Fujita Health University School of Medicine , Aichi , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Fujita Health University School of Medicine , Aichi , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Ohkushi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Cancer Institute Hospital, Japanese Foundation for Cancer Research , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3073-6564","authenticated-orcid":false,"given":"Sohei","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, University of Tokyo Hospital , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Kameda Medical Center , Chiba , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St Luke's International Hospital , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, NTT Medical Center , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuma","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Innovative and Clinical Research Promotion Center, Gifu University Hospital , Gifu , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Sasazawa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Kameda Medical Center , Chiba , Japan"},{"name":"Department of Internal Medicine\/Infectious Diseases, Omachi Municipal General Hospital , Nagano , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeru","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Kameda Medical Center , Chiba , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumi","family":"Takehana","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Cancer Institute Hospital, Japanese Foundation for Cancer Research , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saho","family":"Koyano","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, University of Tokyo Hospital , Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Doi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Fujita Health University School of Medicine , Aichi , Japan"},{"name":"Department of Microbiology, Fujita Health University School of Medicine , Aichi , Japan"},{"name":"Division of Infectious Diseases, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,16]]},"reference":[{"key":"2023122813132083300_ofad587-B1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.00039-09","article-title":"The genus Aeromonas: taxonomy, pathogenicity, and infection","volume":"23","author":"Janda","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"2023122813132083300_ofad587-B2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3390\/microorganisms8010129","article-title":"An update on the genus Aeromonas: taxonomy, epidemiology, and pathogenicity","volume":"8","author":"Fern\u00e1ndez-Bravo","year":"2020","journal-title":"Microorganisms"},{"key":"2023122813132083300_ofad587-B3","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1128\/JCM.41.6.2348-2357.2003","article-title":"The genus Aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes","volume":"41","author":"Abbott","year":"2003","journal-title":"J Clin Microbiol"},{"key":"2023122813132083300_ofad587-B4","doi-asserted-by":"crossref","first-page":"625961","DOI":"10.3389\/fmicb.2020.625961","article-title":"Comparison of the multiple platforms to identify various Aeromonas species","volume":"11","author":"Du","year":"2021","journal-title":"Front Microbiol"},{"key":"2023122813132083300_ofad587-B5","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1099\/ijsem.0.004332","article-title":"List of prokaryotic names with standing in nomenclature (LPSN) moves to the DSMZ","volume":"70","author":"Parte","year":"2020","journal-title":"Int J Syst Evol Microbiol"},{"key":"2023122813132083300_ofad587-B6","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0369-8114(03)00027-0","article-title":"Phenotypes of \u03b2-lactam resistance in the genus Aeromonas [in French]","volume":"51","author":"Fosse","year":"2003","journal-title":"Pathol Biol (Paris)"},{"key":"2023122813132083300_ofad587-B7","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1128\/jb.179.6.2006-2013.1997","article-title":"Expression of the AsbA1, OXA-12, and AsbM1 beta-lactamases in Aeromonas jandaei AER 14 is coordinated by a two-component regulon","volume":"179","author":"Alksne","year":"1997","journal-title":"J Bacteriol"},{"key":"2023122813132083300_ofad587-B8","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1093\/jac\/dkg247","article-title":"Genetic linkage of the penicillinase gene, amp, and blrAB, encoding the regulator of \u03b2-lactamase expression in Aeromonas spp","volume":"51","author":"Niumsup","year":"2003","journal-title":"J Antimicrob Chemother"},{"key":"2023122813132083300_ofad587-B9","doi-asserted-by":"crossref","first-page":"e00543-17","DOI":"10.1128\/mBio.00543-17","article-title":"Novel plasmid-mediated colistin resistance gene mcr-3 in Escherichia coli","volume":"8","author":"Yin","year":"2017","journal-title":"mBio"},{"key":"2023122813132083300_ofad587-B10","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1093\/jac\/dky111","article-title":"Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae","volume":"73","author":"Yang","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2023122813132083300_ofad587-B11","doi-asserted-by":"crossref","first-page":"667406","DOI":"10.3389\/fmicb.2021.667406","article-title":"Prevalence and genetic analysis of chromosomal mcr-3\/7 in Aeromonas from U.S. animal-derived samples","volume":"12","author":"Wang","year":"2021","journal-title":"Front Microbiol"},{"key":"2023122813132083300_ofad587-B12","author":"Clinical and Laboratory Standards Institute."},{"key":"2023122813132083300_ofad587-B13","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1128\/AAC.00866-06","article-title":"Competitive inhibitors of the CphA metallo-\u03b2-lactamase from Aeromonas hydrophila","volume":"51","author":"Horsfall","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"2023122813132083300_ofad587-B14","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.diagmicrobio.2016.02.005","article-title":"Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia","volume":"85","author":"Sinclair","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2023122813132083300_ofad587-B15","doi-asserted-by":"crossref","first-page":"749269","DOI":"10.3389\/fcimb.2021.749269","article-title":"Pancreaticobiliary cancers and Aeromonas isolates carrying type \u2162 secretion system genes ascF-ascG are associated with increased mortality: an analysis of 164 Aeromonas infection episodes in southern Taiwan","volume":"11","author":"Chen","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"2023122813132083300_ofad587-B16","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/jac\/37.3.423","article-title":"Enzyme kinetics and biochemical analysis of ImiS, the metallo-\u03b2-lactamase from Aeromonas sobria 163a","volume":"37","author":"Walsh","year":"1996","journal-title":"J Antimicrob Chemother"},{"key":"2023122813132083300_ofad587-B17","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1007\/s00018-003-3092-x","article-title":"Role of Cys221 and Asn116 in the zinc-binding sites of the Aeromonas 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Chemother"},{"key":"2023122813132083300_ofad587-B23","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0163-4453(95)90808-0","article-title":"Aeromonas septicaemia in Hong Kong species distribution and associated disease","volume":"30","author":"Duthie","year":"1995","journal-title":"J Infect"},{"key":"2023122813132083300_ofad587-B24","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1093\/clind\/15.3.449","article-title":"Epidemiology of Aeromonas infections in California","volume":"15","author":"King","year":"1992","journal-title":"Clin Infect dis"},{"key":"2023122813132083300_ofad587-B25","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/JCM.00155-09","article-title":"Prospective nationwide study of Aeromonas infections in France","volume":"47","author":"Lamy","year":"2009","journal-title":"J Clin Microbiol"},{"key":"2023122813132083300_ofad587-B26","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1086\/382876","article-title":"Outbreak of Aeromonas hydrophila wound infections associated with mud football","volume":"38","author":"Vally","year":"2004","journal-title":"Clin Infect Dis"},{"key":"2023122813132083300_ofad587-B27","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1139\/W07-051","article-title":"Virulence potential and genetic diversity of Aeromonas caviae, Aeromonas veronii, and Aeromonas hydrophila clinical isolates from Mexico and Spain: a comparative study","volume":"53","author":"Aguilera-Arreola","year":"2007","journal-title":"Can J Microbiol"},{"key":"2023122813132083300_ofad587-B28","doi-asserted-by":"crossref","first-page":"1084352","DOI":"10.3389\/fcimb.2023.1084352","article-title":"Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from 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carbapenemase-producing Aeromonas","volume":"14","author":"Wang","year":"2021","journal-title":"Infect Drug Resist"},{"key":"2023122813132083300_ofad587-B35","doi-asserted-by":"crossref","first-page":"793","DOI":"10.3389\/fmicb.2016.00793","article-title":"Aeromonas dhakensis, an increasingly recognized human pathogen","volume":"7","author":"Chen","year":"2016","journal-title":"Front Microbiol"},{"key":"2023122813132083300_ofad587-B36","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1093\/jac\/dky262","article-title":"Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes","volume":"73","author":"Partridge","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2023122813132083300_ofad587-B37","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1093\/jac\/dkx538","article-title":"Identification of novel variants of the colistin resistance gene mcr-3 in Aeromonas spp. From the national resistance monitoring programme GERM-vet and from diagnostic submissions","volume":"73","author":"Eichhorn","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2023122813132083300_ofad587-B38","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1046\/j.1472-765X.2003.01257.x","article-title":"Induced colistin resistance as an identifying marker for Aeromonas phenospecies groups","volume":"36","author":"Fosse","year":"2003","journal-title":"Lett Appl Microbiol"},{"key":"2023122813132083300_ofad587-B39","doi-asserted-by":"crossref","first-page":"109135","DOI":"10.1016\/j.celrep.2021.109135","article-title":"The MCR-3 inside linker appears as a facilitator of colistin resistance","volume":"35","author":"Xu","year":"2021","journal-title":"Cell Rep"}],"container-title":["Open Forum Infectious 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