{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T09:03:41Z","timestamp":1792746221772,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Niger's National Malaria Control Programme and its partners use histidine-rich protein 2\u2013based RDTs, which are specific to Plasmodium falciparum diagnosis. This study aimed to screen for the circulation of non-falciparum species in Zinder, a region of Niger, West Africa.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A cross-sectional study was carried out from July to December 2022 at the district hospital of the Zinder region of Niger. P falciparum histidine-rich protein 2\u2013based rapid diagnostic tests were performed, and dried blood spot samples were collected for further laboratory multiplexed photo-induced electron transfer\u2013polymerase chain reaction (PET-PCR) analysis on positive light microscopy from all patients with fever who attended the Zinder district hospital during the study period.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In total, 340 dried blood spots were collected and analyzed by PET-PCR. Overall, 73.2% (95% CI, 68.2%\u201377.9%; 249\/340) were positive for Plasmodium genus and species and represented the study population. Plasmodium species proportions were 89.5% (95% CI, 85.1%\u201393.1%; 223\/249) for P falciparum, 38.5% (95% CI, 32.5%\u201344.9%; 96\/249) for P malariae, 10.8% (95% CI, 7.3%\u201315.4%; 27\/249) for P vivax, and 1.6% (95% CI, .4%\u20134.1%; 4\/249) for P ovale. Single infection with Plasmodium species counted for 61.8% (95% CI, 55.5%\u201367.9%; 154\/249), and the mixed infections rate, with at least 2 Plasmodium species, was 38.1% (95% CI, 32.1%\u201344.5%; 95\/249). Single non-falciparum infections represented a rate of 10.0% (95% CI, 6.6%\u201314.5%; 25\/249).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This study confirms the first evidence of Plasmodium vivax by PET-PCR in Niger in addition to the other 3 Plasmodium species. These findings underline the need to adapt malaria diagnostic tools and therapeutic management, as well as the training of microscopists, for recognition of non-falciparum plasmodial species circulating in the country. This will better inform the strategies toward malaria control and elimination, as well as the decision making of the health authorities of Niger.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofae474","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T06:37:26Z","timestamp":1723790246000},"source":"Crossref","is-referenced-by-count":5,"title":["Circulation of Non-<i>falciparum<\/i> Species in Niger: Implications for Malaria Diagnosis"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1277-7696","authenticated-orcid":false,"given":"Mamane N","family":"Garba","sequence":"first","affiliation":[{"name":"Centre International de Recherche et de Formation en G\u00e9nomique Appliqu\u00e9e et de Surveillance Sanitaire, Facult\u00e9 de M\u00e9decine, de Pharmacie et d\u2019Odontologie, Universit\u00e9 Cheikh Anta Diop de Dakar ,","place":["S\u00e9n\u00e9gal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5384-546X","authenticated-orcid":false,"given":"Lamine","family":"M. 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