{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T11:14:38Z","timestamp":1781090078937,"version":"3.54.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"vor","delay-in-days":4,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>This study was conducted to evaluate screening procedures for anal high-grade squamous intraepithelial lesions (HSILs) with anal liquid-based cytology (aLBC) and biomarkers to identify candidates for high-resolution anoscopy (HRA).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This cross-sectional study included men who have sex with men with HIV. Participants underwent HRA, aLBC, and biomarker testing. Three screening procedures were compared with aLBC: biomarker alone, cytology and biomarker in all, and cytology and reflex biomarkers (biomarkers applied if aLBC results were atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion). Biomarkers included Linear Array (LA), LA for 14 high-risk human papillomavirus (LA 14 HR-HPV) genotypes, LA HPV-16, Hybrid Capture 2 (HC2), E6\/E7 mRNA, and E6\/E7 mRNA HPV-16.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 354 participants, 179 (50.6%) had atypical squamous cells of undetermined significance or worse, requiring HRA (sensitivity, 80%; specificity, 57.3%; area under the curve, 0.687; reference, biopsy-proven HSIL). Cytology and reflex biomarkers per E6\/E7 mRNA, LA 14 HR-HPV, and HC2 and the biomarker-alone procedure with HC2 showed comparable accuracy (sensitivities: 71.6%, 78.8%, 73.1%, 75.7%; specificities: 73.5%, 67.9%, 76.1%, 65.5%; areas under the curve: 0.726, 0.734, 0.746, 0.706) with fewer HRA referrals (number needed to diagnose: 2.2, 2.1, 2, 2.4).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings suggest that E6\/E7 mRNA, LA 14 HR-HPV, and HC2 in the cytology and reflex biomarkers procedure, as well as HC2 in the biomarker-alone procedure, can improve anal HSIL screening effectiveness.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofae735","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T05:21:02Z","timestamp":1735276862000},"source":"Crossref","is-referenced-by-count":3,"title":["Using Anal Cytology and Human Papillomavirus DNA and E6\/E7 mRNA Detection to Optimize High-Resolution Anoscopy Referrals in Men Who Have Sex With Men With HIV"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4660-534X","authenticated-orcid":false,"given":"Ana C","family":"Silva-Klug","sequence":"first","affiliation":[{"name":"HIV and STD Unit, Infectious Diseases Department, Bellvitge University Hospital\/Bellvitge Biomedical Research Institute, L\u2019Hospitalet de Llobregat , Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f2nia","family":"Paytubi","sequence":"additional","affiliation":[{"name":"Infection and Cancer Laboratory, Cancer Epidemiology Research Program, Catalan Institute of Oncology\/Bellvitge Biomedical Research Institute, L\u2019Hospitalet de Llobregat , Barcelona ,","place":["Spain"]},{"name":"Centro de investigaci\u00f3n Biom\u00e9dica en Red en Epidemiolog\u00eda y Salud P\u00fablica, Ministerio de Ciencia e 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,","place":["Spain"]},{"name":"Centro de investigaci\u00f3n Biom\u00e9dica en Red en Epidemiolog\u00eda y Salud P\u00fablica, Ministerio de Ciencia e Innovaci\u00f3n en Epidemiolog\u00eda y Salud P\u00fablica , Madrid ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"De Sanjose","sequence":"additional","affiliation":[{"name":"Viral and Bacterial Infections Worldwide Program, Barcelona Institute for Global Health , Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Catala","sequence":"additional","affiliation":[{"name":"Pathology Unit, Bellvitge University Hospital\/Bellvitge Biomedical Research Institute, L\u2019Hospitalet de Llobregat , Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"August","family":"Vidal","sequence":"additional","affiliation":[{"name":"Pathology Unit, Bellvitge University Hospital\/Bellvitge Biomedical 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