{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:25:00Z","timestamp":1768285500682,"version":"3.49.0"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/8\/5\/7\/Adhoc\/2020\/ECD-I"],"award-info":[{"award-number":["5\/8\/5\/7\/Adhoc\/2020\/ECD-I"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>We recently described the utility of the \u201cTB Concentration &amp; Transport\u201d kit for biosafe, ambient temperature transport of dried sputum on Trans-Filter, and the \u201cTB DNA Extraction\u201d kit for DNA extraction from Trans-Filter for the early diagnosis of drug-resistant tuberculosis (TB). This study aimed to assess the feasibility and compatibility of these kits with line probe assay (LPA) under National Tuberculosis Elimination Programme (NTEP) settings.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Patients with presumptive pulmonary TB, multidrug-resistant (MDR) TB, or extensively drug-resistant TB (N = 8491) who attended Designated Microscopy Centers (DMCs, n = 13) under National Reference Laboratories (NRLs) at Bhopal, New Delhi, Chennai, and Bhubaneswar were screened by smear microscopy. The performance of Trans-Filter extracted DNA\u2013based LPA (Kit-LPA) was assessed against Direct-LPA on smear-positive sputum (n = 681), and feedback was obtained from scientists (n = 10) and laboratory technicians (n = 42) regarding logistics, kit usage, training, and troubleshooting. A scoring questionnaire was used to assess (i) the TB Concentration &amp; Transport kit versus conventional sputum transport and (ii) the TB DNA Extraction kit versus Hain GenoLyse kit (statistical significance of the scores was calculated using paired t test).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Kit-LPA showed a sensitivity and specificity in the range of 89%\u201396% and 99%\u2013100%, respectively, for rifampicin and isoniazid resistance detection and was comparable to Direct-LPA (concordance = 99%\u2013100%; \u03ba = 0.94\u20130.97). Overall scores indicated that (i) sputum transport on Trans-Filters was more convenient as compared to conventional sputum transport (P &amp;lt; .0001) and (ii) Trans-Filter extracted DNA was easily amalgamated with LPA testing for MDR-TB detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These findings provide evidence for incorporation of these kits into the NTEP. Their use can facilitate sputum transport from DMCs to NRLs and provide universal drug susceptibility testing to people with TB residing in remote areas.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofaf207","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T03:17:26Z","timestamp":1744341446000},"source":"Crossref","is-referenced-by-count":1,"title":["Operational Feasibility of \u201cTB Concentration &amp; Transport\u201d and \u201cTB DNA Extraction\u201d Kits for Biosafe Dry Sputum Transport for Direct Detection of Drug Resistance Using Line Probe Assay: A Field Study in India"],"prefix":"10.1093","volume":"12","author":[{"given":"Keerti","family":"Chauhan","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh Kumar","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9039-6420","authenticated-orcid":false,"given":"Divya","family":"Anthwal","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2651-0132","authenticated-orcid":false,"given":"Nikita","family":"Panwalkar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Bhopal Memorial Hospital and Research Centre , Bhopal ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabha","family":"Desikan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Bhopal Memorial Hospital and Research Centre , Bhopal ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2166-5760","authenticated-orcid":false,"given":"Ritu","family":"Singhal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases , New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manpreet","family":"Bhalla","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases , New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vithal Prasad","family":"Myneedu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases , New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid Umar","family":"Khayyam","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases , New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9203-2664","authenticated-orcid":false,"given":"Siva Kumar","family":"Shanmugam","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Indian Council of Medical Research (ICMR)-National Institute for Research in Tuberculosis , Chennai ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K Silambu","family":"Chelvi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Indian Council of Medical Research (ICMR)-National Institute for Research in Tuberculosis , Chennai ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1324-4595","authenticated-orcid":false,"given":"Padmapriyadarsini","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Indian Council of Medical Research (ICMR)-National Institute for Research in Tuberculosis , Chennai ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-7392","authenticated-orcid":false,"given":"Sidhartha","family":"Giri","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Tuberculosis, ICMR-Regional Medical Research Centre , Bhubaneshwar ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyotirmayee","family":"Turuk","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Tuberculosis, ICMR-Regional Medical Research Centre , Bhubaneshwar ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dasarathi","family":"Das","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Tuberculosis, ICMR-Regional Medical Research Centre , Bhubaneshwar ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-5592","authenticated-orcid":false,"given":"Sanghamitra","family":"Pati","sequence":"additional","affiliation":[{"name":"National Reference Laboratory (NRL) for Tuberculosis, ICMR-Regional Medical Research Centre , Bhubaneshwar ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhinav","family":"Goyal","sequence":"additional","affiliation":[{"name":"Advanced Microdevices Pvt Ltd , Ambala Cantt ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashawant","family":"Gupta","sequence":"additional","affiliation":[{"name":"Advanced Microdevices Pvt Ltd , Ambala Cantt ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nalini Kant","family":"Gupta","sequence":"additional","affiliation":[{"name":"Advanced Microdevices Pvt Ltd , Ambala Cantt ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjula","family":"Singh","sequence":"additional","affiliation":[{"name":"India Tuberculosis Research Consortium, Indian Council of Medical Research, V. Ramalingaswami Bhawan , Ansari Nagar, New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6052-1137","authenticated-orcid":false,"given":"Jaya Sivaswami","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, All India Institute of Medical Sciences , Ansari Nagar, New Delhi ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8276-4458","authenticated-orcid":false,"given":"Sagarika","family":"Haldar","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"2025072211013749100_ofaf207-B1","author":"World Health Organization"},{"key":"2025072211013749100_ofaf207-B2","author":"World Health Organization"},{"key":"2025072211013749100_ofaf207-B3","author":"National Tuberculosis Elimination Programme"},{"key":"2025072211013749100_ofaf207-B4","doi-asserted-by":"crossref","first-page":"e0220967","DOI":"10.1371\/journal.pone.0220967","article-title":"Development and evaluation of novel bio-safe filter paper\u2013based kits for sputum microscopy and transport to directly detect Mycobacterium tuberculosis and associated drug resistance","volume":"14","author":"Anthwal","year":"2019","journal-title":"PLoS One"},{"key":"2025072211013749100_ofaf207-B5","doi-asserted-by":"crossref","first-page":"00137-2021","DOI":"10.1183\/23120541.00137-2021","article-title":"Compatibility of a novel filter paper\u2013based bio-safe sputum transport kit with line probe assay for diagnosing drug-resistant tuberculosis: a single-site evaluation study","volume":"7","author":"Anthwal","year":"2021","journal-title":"ERJ Open Res"},{"key":"2025072211013749100_ofaf207-B6","author":"Revised National Tuberculosis Control Programme"},{"key":"2025072211013749100_ofaf207-B7","author":"Revised National Tuberculosis Control Programme"},{"key":"2025072211013749100_ofaf207-B8","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1080\/23744235.2024.2375599","article-title":"Operational feasibility and multi-centric evaluation of \u2018TBDetect sputum microscopy kit\u2019 for the direct detection of Mycobacterium tuberculosis in field settings","volume":"56","author":"Chauhan","year":"2024","journal-title":"Infect Dis (Lond)"},{"key":"2025072211013749100_ofaf207-B9","doi-asserted-by":"crossref","first-page":"911.e1","DOI":"10.1016\/j.cmi.2020.08.020","article-title":"Evaluation of \u2018TBDetect\u2019 sputum microscopy kit for improved detection of Mycobacterium tuberculosis: a multi-centric validation study","volume":"27","author":"Anthwal","year":"2021","journal-title":"Clin Microbiol Infect"},{"key":"2025072211013749100_ofaf207-B10","author":"Global Laboratory Initiative"},{"key":"2025072211013749100_ofaf207-B11","author":"Hain Lifesciences"},{"key":"2025072211013749100_ofaf207-B12","author":"World Health Organization"}],"container-title":["Open Forum Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ofid\/advance-article-pdf\/doi\/10.1093\/ofid\/ofaf207\/62909270\/ofaf207.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ofid\/article-pdf\/12\/7\/ofaf207\/62909270\/ofaf207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ofid\/article-pdf\/12\/7\/ofaf207\/62909270\/ofaf207.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T15:01:43Z","timestamp":1753196503000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ofid\/article\/doi\/10.1093\/ofid\/ofaf207\/8110869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,6,30]]}},"URL":"https:\/\/doi.org\/10.1093\/ofid\/ofaf207","relation":{},"ISSN":["2328-8957"],"issn-type":[{"value":"2328-8957","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,7]]},"published":{"date-parts":[[2025,4,11]]},"article-number":"ofaf207"}}