{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T20:33:57Z","timestamp":1785443637819,"version":"3.56.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008459","name":"University of Calgary","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>According to the World Health Organization, almost two-thirds of the Ethiopian population is at risk of contracting malaria, where infection with Plasmodium falciparum accounts for approximately 60% of cases today. The risk of artemisinin resistance spreading from southeast Asia to Africa is a major concern. We conducted a 28-day in vivo efficacy trial of artemether-lumefantrine (Coartem) for treatment of uncomplicated malaria (n = 97) in the Gondar area, northwest Ethiopia, from 2017 to 2018. Our results confirmed 100% adequate clinical and parasitologic response, with no parasites observed at day 3 by microscopy. Further analysis of day 0 samples showed the expansion of a Kelch13 mutation R622I to 9.5% from 2.4% of isolates reported 3 years earlier. Closer examination of the R622I mutation in vitro is warranted.<\/jats:p>","DOI":"10.1093\/ofid\/ofaf279","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:01:31Z","timestamp":1746748891000},"source":"Crossref","is-referenced-by-count":6,"title":["Expansion of the <i>Plasmodium falciparum Kelch13<\/i> R622I Mutation in Northwest Ethiopia"],"prefix":"10.1093","volume":"12","author":[{"given":"Aberham Abere","family":"Alemayehu","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology, School of Biomedical & Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar , Gondar ,","place":["Ethiopia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Casta\u00f1eda Mogoll\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medicine, and Microbiology Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisay Getie","family":"Belay","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, School of Biomedical & Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar , Gondar ,","place":["Ethiopia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habtie","family":"Tesfa","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, School of Biomedical & Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar , Gondar ,","place":["Ethiopia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abu Naser","family":"Mohon","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medicine, and Microbiology Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirujah","family":"Balasingam","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medicine, and Microbiology Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abebe Genetu","family":"Bayih","sequence":"additional","affiliation":[{"name":"Africa Centers for Disease Control , Addis Ababa ,","place":["Ethiopia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoaib","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medicine, and Microbiology Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan R","family":"Pillai","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medicine, and Microbiology Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,5,9]]},"reference":[{"key":"2025062505200845900_ofaf279-B1","doi-asserted-by":"crossref","DOI":"10.30875\/9789287076717","volume-title":"World malaria report 2024: addressing inequity in the global malaria response","author":"World Health Organization","year":"2024"},{"key":"2025062505200845900_ofaf279-B2","doi-asserted-by":"crossref","first-page":"17095","DOI":"10.1038\/s41598-019-53386-w","article-title":"The burden of submicroscopic and asymptomatic malaria in India revealed from epidemiology studies at three varied transmission sites in India","volume":"9","author":"van Eijk","year":"2019","journal-title":"Sci Rep"},{"key":"2025062505200845900_ofaf279-B3","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/S1473-3099(18)30420-1","article-title":"HRP-2 deletion: a hole in the ship of malaria elimination","volume":"18","author":"Verma","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"2025062505200845900_ofaf279-B4","volume-title":"World malaria report 2023","author":"World Health Organization","year":"2023"},{"key":"2025062505200845900_ofaf279-B5","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1016\/S0140-6736(05)67787-2","article-title":"Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6","volume":"366","author":"Jambou","year":"2005","journal-title":"Lancet"},{"key":"2025062505200845900_ofaf279-B6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1365-3156.2005.01557.x","article-title":"In vivo sensitivity monitoring of mefloquine monotherapy and artesunate-mefloquine combinations for the treatment of uncomplicated falciparum malaria in Thailand in 2003","volume":"11","author":"Vijaykadga","year":"2006","journal-title":"Trop Med Int Health"},{"key":"2025062505200845900_ofaf279-B7","doi-asserted-by":"publisher","author":"Brhane","DOI":"10.1101\/2024.09.11.24313421"},{"key":"2025062505200845900_ofaf279-B8","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1038\/s41564-023-01461-4","article-title":"Plasmodium falciparum resistant to artemisinin and diagnostics have emerged in Ethiopia","volume":"8","author":"Fola","year":"2023","journal-title":"Nat 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Polymorphisms of pfcoronin in natural populations: implications for functional significance","volume":"116","author":"Sharma","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2025062505200845900_ofaf279-B10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/jb\/mvab070","article-title":"Functional analyses of Plasmodium ferredoxin Asp97Tyr mutant related to artemisinin resistance of human malaria parasites","volume":"170","author":"Kimata-Ariga","year":"2021","journal-title":"J Biochem"},{"key":"2025062505200845900_ofaf279-B11","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/ng.3189","article-title":"Genetic architecture of artemisinin-resistant Plasmodium falciparum","volume":"47","author":"Miotto","year":"2015","journal-title":"Nat Genet"},{"key":"2025062505200845900_ofaf279-B12","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4269\/ajtmh.15-0477","article-title":"A unique Plasmodium falciparum K13 gene mutation in northwest 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