{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T14:44:14Z","timestamp":1758811454443,"version":"3.44.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":26,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Biotechnology Research and Innovation Council-National Institute of Biomedical Genomics"},{"name":"Senior Research Fellowship"},{"DOI":"10.13039\/501100001412","name":"Council of Scientific & Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Neonates are highly susceptible to sepsis, which is often accompanied by fatal coagulopathy. Anticoagulant therapies have not reduced sepsis-related mortality in clinical trials, possibly due to patient heterogeneity. Neutrophil extracellular traps (NETs) enhance coagulation by activating platelets, suggesting that NET-specific biomarkers may identify patients who may benefit from targeted anticoagulant treatment. This study evaluated the association between NET gene expression and adverse outcomes in neonatal sepsis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We analyzed whole blood transcriptomes from 123 neonates with sepsis and developed a predictive model, the NET score, based on NET-related gene expression. Model performance was assessed in two independent validation sets. Mediation and correlation analyses explored the relationship between the NET score and a coagulation score. Temporal transcriptomic data from septic shock cases further tested this interaction.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The NET score achieved AUCs of 88.7% and 85.4% in validation Sets 1 and 2, respectively, indicating strong predictive performance. Mediation and temporal analyses supported a sequential relationship between NETosis and coagulation in sepsis. Age-specificity of the model was confirmed using pediatric (n = 163) and adult (n = 86) sepsis transcriptomic datasets. Neonates with disseminated intravascular coagulation exhibited a trend toward elevated NET scores.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings support a novel risk stratification approach using the NET score to identify neonates at increased risk for sepsis-associated coagulopathy and poor outcomes, potentially guiding targeted therapeutic strategies.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofaf570","type":"journal-article","created":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T11:39:37Z","timestamp":1758454777000},"source":"Crossref","is-referenced-by-count":0,"title":["Examining Transcriptomic Markers Associated With Neutrophil Extracellular Traps to Predict Mortality Risk in Neonatal Sepsis"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6883-2237","authenticated-orcid":false,"given":"Deepshikha","family":"Shaw","sequence":"first","affiliation":[{"name":"Biotechnology Research and Innovation Council, National Institute of Biomedical Genomics (BRIC-NIBMG) , Kalyani, West Bengal ,","place":["India"]},{"name":"Regional Centre for Biotechnology (RCB) , Faridabad, Haryana ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sridhar","family":"Santhanam","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Christian Medical College, Vellore , Vellore, Tamil Nadu ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8356-9125","authenticated-orcid":false,"given":"Tapas Kumar","family":"Som","sequence":"additional","affiliation":[{"name":"Department of Neonatology, All India Institute of Medical Sciences Bhubaneswar , Bhubaneswar, Odisha ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8549-1130","authenticated-orcid":false,"given":"Samsiddhi","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Biotechnology Research and Innovation Council, National Institute of Biomedical Genomics (BRIC-NIBMG) , Kalyani, West Bengal ,","place":["India"]},{"name":"Regional Centre for Biotechnology (RCB) , Faridabad, Haryana ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4723-5109","authenticated-orcid":false,"given":"Saroj Kant","family":"Mohapatra","sequence":"additional","affiliation":[{"name":"Biotechnology Research and Innovation Council, National Institute of Biomedical Genomics (BRIC-NIBMG) , Kalyani, West Bengal ,","place":["India"]},{"name":"Regional Centre for Biotechnology (RCB) , Faridabad, Haryana 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