{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T02:46:14Z","timestamp":1784774774520,"version":"3.55.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019748","name":"Johns Hopkins University","doi-asserted-by":"publisher","award":["NIBIB U54EB007958"],"award-info":[{"award-number":["NIBIB U54EB007958"]}],"id":[{"id":"10.13039\/100019748","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"publisher","award":["UM1AI068613"],"award-info":[{"award-number":["UM1AI068613"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Point-of-care (POC) polymerase chain reaction (PCR) tests for sexually transmitted infections (STIs) represent a potential paradigm shift for emergency department (ED) management of patients with suspected STIs, given there are now Food and Drug Administration\u2013cleared POC tests that permit definite rapid diagnosis and result-driven care.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A quasi-experimental real-world implementation study was conducted in an urban ED, comparing two approaches for female STI testing for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Trichomonas vaginalis (TV): (1) central laboratory testing (August\u2013November 2022) with batched nucleic acid amplification testing (CT\/NG) and wet prep for TV; (2) POC PCR Testing Integration (ED POC) (January\u2013April 2023) in an ED POC laboratory for all three STIs. We compared proportions of appropriate treatment and ED length of stay (LOS) between the two testing modalities using chi-square test and log-transformed multivariable linear regression, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 627 patients, 340 received central laboratory testing and 287 received ED POC; ED POC resulted in a significant decrease in LOS by 76\u2005minutes or 9.3% (95% confidence interval [CI], \u221216.3% to \u22121.7%; P = .017). ED POC also significantly lowered overtreatment rates for CT (n = 595) and NG (n = 607) by 73% (95% CI, 44\u201387; P &amp;lt; .001) and 63% (95% CI, 28\u201381; P = .002), respectively. ED POC testing was associated with 67% lower rate of undertreatment (95% CI, \u221219% to 91%; P = .093) for any CT\/NG\/TV-positive (n = 78), but not statistically significant due to relatively small number of undertreated cases .<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Compared to traditional STI testing, POC PCR testing significantly shortened ED LOS, allowed for organism-specific targeted treatment, and reduced overtreatment of CT and NG infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofaf749","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T13:16:45Z","timestamp":1765372605000},"source":"Crossref","is-referenced-by-count":2,"title":["Real-world Use of Molecular Point-of-care Testing for Sexually Transmitted Infections (STIs) in the Emergency Department: Why It Matters for Acute Care Management"],"prefix":"10.1093","volume":"13","author":[{"given":"Gaby","family":"Dashler","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendall","family":"Maliszewski","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustapha","family":"Saheed","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edana","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nyah","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spencer J","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Colburn","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Pathology, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte A","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari C","family":"Manabe","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K Davina","family":"Frick","sequence":"additional","affiliation":[{"name":"Department of Operations Management and Business Analytics, Carey Business School, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard E","family":"Rothman","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]},{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1616-8014","authenticated-orcid":false,"given":"Yu-Hsiang","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, School of Medicine, Johns Hopkins University , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"2026010203312322600_ofaf749-B1","first-page":"1","article-title":"Emergency department visit rates by selected characteristics: United States, 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