{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T16:01:37Z","timestamp":1771344097608,"version":"3.50.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":3,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institutes for Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI155730"],"award-info":[{"award-number":["R01AI155730"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institutes for Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K24AI134990"],"award-info":[{"award-number":["K24AI134990"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The Zanzibar archipelago has been a pre-elimination region for malaria thanks to rigorous control interventions, but recent surges in malaria cases have been observed. The contribution of non-falciparum species to the current malaria situation is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>This study investigates the epidemiology of falciparum and non-falciparum malaria species in Zanzibar. Leveraging plasma extracted from dried blood spots (DBS) collected during reactive case detection (RCD) activities across Unguja island from May 2022 to January 2023, we measured immunoglobulin G (IgG) responses to Plasmodium MSP1-19kD antigens using a multiplex bead-based immunoassay. Additionally, active infections were detected using species-specific real-time PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Out of 1618 participants surveyed in 35 RCDs, 35.3% had exposure to any malaria species, with Plasmodium falciparum being the most common (29.8%). Seroprevalences for non-falciparum species were lower: Plasmodium ovale (5.8%), Plasmodium malariae (5.9%), and Plasmodium vivax (5.9%). Active infections were detected in 6.0% of participants, predominantly P. falciparum (4.6%). Travel to mainland Tanzania was a dominant risk factor for seropositivity for all 4 malaria species. Other factors associated with Pf seropositivity (high-risk occupations and female status) were not associated with seropositivity for non-falciparum species. The geographic distribution of non-falciparum exposure differed compared to falciparum, with relatively higher seroprevalences in rural districts, especially Kazkazini A in northern Unguja.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study suggests a significant contribution of non-falciparum species to the local epidemiology in Zanzibar. Current control and elimination efforts, focused on P. falciparum, may not adequately address exposure to non-falciparum species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofag051","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:41:37Z","timestamp":1770122497000},"source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Epidemiology of Malaria in Zanzibar Through Molecular and Serological Analysis of Samples Collected During Reactive Case Detection"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2933-427X","authenticated-orcid":false,"given":"Varun","family":"Goel","sequence":"first","affiliation":[{"name":"Department of Geography, University of South Carolina , Columbia, South Carolina 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,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8338-3315","authenticated-orcid":false,"given":"Mohamed","family":"Ali","sequence":"additional","affiliation":[{"name":"Zanzibar Malaria Elimination Program (ZAMEP) , Zanzibar ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bakari","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Zanzibar Malaria Elimination Program (ZAMEP) , Zanzibar ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9380-6158","authenticated-orcid":false,"given":"Abdallah","family":"Zacharia","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Medical Entomology, Muhimbili University of Health and Allied Sciences , Dar es Salaam ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Msolo C","family":"Dominick","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Medical Entomology, Muhimbili University of Health and Allied Sciences , Dar es Salaam ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyaw","family":"Thwai","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safia","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Zanzibar Malaria Elimination Program (ZAMEP) , Zanzibar ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shija J","family":"Shija","sequence":"additional","affiliation":[{"name":"Zanzibar Malaria Elimination Program (ZAMEP) , Zanzibar ,","place":["Tanzania"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6899-8204","authenticated-orcid":false,"given":"Jeffrey A","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Brown University , Providence, Rhode Island ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8882-130X","authenticated-orcid":false,"given":"Anders","family":"Bj\u00f6rkman","sequence":"additional","affiliation":[{"name":"Department of Global Public Health, Karolinska Institute , Stockholm ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9300-7719","authenticated-orcid":false,"given":"Billy E","family":"Ngasala","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Medical Entomology, Muhimbili University of Health and Allied Sciences , Dar es Salaam ,","place":["Tanzania"]},{"name":"Department of Women's and Children's Health, International Maternal and Child Health (IMCH), Uppsala University , Uppsala ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7375-8304","authenticated-orcid":false,"given":"Eric","family":"Rogier","sequence":"additional","affiliation":[{"name":"Division of Digestive Disease and Nutrition, University of Kentucky , Lexington, Kentucky ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0591-0850","authenticated-orcid":false,"given":"Jonathan J","family":"Juliano","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina , Chapel Hill, North Carolina ,","place":["USA"]},{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina at 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