{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:00:31Z","timestamp":1785405631754,"version":"3.56.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pharyngitis is a frequent cause of urgent care (UC) visits. The SPOTFIRE Sore Throat (SPOTFIRE ST) panel is a Clinical Laboratory Improvement Amendments\u2013waived point-of-care test capable of detecting 14 common bacterial and viral pathogens from an oropharyngeal swab. We evaluated detection characteristics, antimicrobial prescribing, and clinician and patient perceptions associated with SPOTFIRE ST testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a prospective, single-center study at an academic UC clinic. Eligible participants included 1\u20132-year-olds with fever, 3\u201317-year-olds with acute pharyngitis, and adult patients with acute pharyngitis and either immunocompromising or comorbid conditions. Clinical variables were collected, and specimens were tested according to SPOTFIRE ST instructions. Clinicians completed a pre-implementation survey, and clinicians and patients completed a postimplementation survey to assess perceptions and satisfaction with the test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>At least 1 detected pathogen occurred in 149\/200 (74.5%) patients. Compared with adults, pediatric patients had higher detections (85% vs 60%; P &amp;lt; .001), statistically significantly higher detections of specifically Group A Streptococcus, influenza B, and M. pneumoniae, and higher detections of a pathogen with specific antimicrobial treatment (68% vs 28%; P &amp;lt; .001). Antibiotics were prescribed in only 7% of cases when a virus was detected. Stakeholders reported high satisfaction with SPOTFIRE ST testing and thought it useful in guiding clinical decisions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SPOTFIRE ST testing demonstrated a high proportion of detections with distinct differences between pediatric and adult patients with acute pharyngitis. The detection characteristics and high satisfaction indicate that this test is likely to be valued by clinicians and patients and may improve clinical assessment and antimicrobial prescribing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Trials Registration<\/jats:title>\n                    <jats:p>ClinicalTrials.gov identifier. NCT06713642.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofag441","type":"journal-article","created":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T11:33:28Z","timestamp":1784460808000},"source":"Crossref","is-referenced-by-count":0,"title":["Point-of-Care Multiplex NAAT for Acute Pharyngitis in the Urgent Care: Detection Patterns, Associated Antimicrobial Prescribing, and Stakeholder Perceptions"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9299-3027","authenticated-orcid":false,"given":"Alexander J","family":"Lepak","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0599-5196","authenticated-orcid":false,"given":"Brittany J","family":"Lehrer","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shari","family":"Barlow","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen","family":"Goss","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Hamer","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3529-8906","authenticated-orcid":false,"given":"Cecilia","family":"He","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Norton","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Temte","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Walters","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7097-583X","authenticated-orcid":false,"given":"Jonathan L","family":"Temte","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Community Health, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"2026073005165752900_ofag441-B1","first-page":"1","article-title":"Ambulatory medical care utilization estimates for 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