{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:20:20Z","timestamp":1787854820847,"version":"build-2784847793"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Defining the microbial etiology of sepsis is difficult but has important implications for antibiotic stewardship and prognosis. We explore the diagnostic yield of a novel, direct-from-blood multiplex polymerase chain reaction in intensive care unit patients with suspected sepsis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From March 2022 to March 2024, patients were included if the clinical team ordered a blood culture. In study participants, an additional 6\u2005mL of whole blood was collected by the phlebotomist. Samples were analyzed by the RaPID\/BSI platform, a polymerase chain reaction-based assay that detects 19 bacterial and fungal species direct from whole blood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 165 samples were included in the final cohort. Of these, 27 patients had a positive RaPID\/BSI result and 8 patients had positive blood cultures with 1 pathogen not included in the RaPID\/BSI panel. Of the 27 patients with positive RaPID\/BSI results, 5 patients also had positive blood cultures. Pathogen identification from RaPID\/BSI and blood cultures were concordant for 4 of 5 of these pathogens. Twenty-five of the 27 RaPID\/BSI positive patients had clinically defined infections. Eighteen patients of the 27 RaPID\/BSI-positive patients had additional positive microbial culture data that corroborated pathogen identification. Of the 27 patients with bloodstream infections detected by RaPID\/BSI and\/or blood cultures, 20 had received antibiotics in the 48\u2005hours before their blood draw. The RaPID\/BSI assay identified the pathogen in all 20 of these patients, whereas only 4 of these 20 patients had positive blood cultures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The diagnostic yield of a direct-from-blood molecular diagnostic assay for the detection of bloodstream infection was 15.2% compared to 4.3% from blood cultures, providing higher detection rate especially in the setting of recent antibiotic administration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofag486","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T11:53:53Z","timestamp":1786190033000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a Direct-from-Blood Molecular Diagnostic Platform for Pathogen Detection in Critically Ill Patients: A Prospective Observational Cohort Study"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7254-5697","authenticated-orcid":false,"given":"Yupeng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Northwestern University Feinberg School of Medicine , Chicago, Illinois ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Donnelly","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine , Chicago, Illinois ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"Donayre","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine , Chicago, Illinois 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