{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:48:11Z","timestamp":1792399691473,"version":"4.4.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"United States Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["75D30121D12750"],"award-info":[{"award-number":["75D30121D12750"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There are limited data on the molecular epidemiology of New Delhi metallo-\u03b2-lactamase (NDM)\u2013producing carbapenem-resistant Enterobacterales (CRE) in Latin America, despite the high burden of NDM-CRE in the region. This study characterized blaNDM-harboring plasmids from CRE from patients and the environment of an Argentine hospital.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>We performed whole-genome sequencing of 40 environmental isolates (Klebsiella complex, n = 11; Enterobacter cloacae, complex n = 16; Citrobacter freundii, n = 10; Serratia marcescens, n = 3) and 4 clinical isolates (Klebsiella pneumoniae, n = 4) recovered from the environment and rectal surveillance samples of patients in the same intensive care units (ICUs) at a tertiary care center in Argentina, July 2023\u2013February 2024, respectively. Representative genomic groups underwent Oxford Nanopore sequencing and hybrid genome assembly for complete plasmid characterization. Resolved blaNDM-harboring plasmids were compared across species and publicly available reference sequences to characterize plasmid relatedness and dissemination patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ten representative genomic groups were identified across species, including 7 defined sequence types and 3 assigned in this study. blaNDM-1 and blaNDM-5 were present in 72.5% (29\/40) and 27.5% (11\/40) of environmental isolates, respectively (all 4 clinical isolates were blaNDM-5). Hybrid assembly of the representative genomic groups revealed blaNDM-1 was located on highly conserved plasmids belonging to the IncC incompatibility group across multiple CRE species. blaNDM-5 was restricted to Klebsiella spp. and was associated with structurally diverse plasmids including IncF- and IncHI1B\/IncFIB-type backbones in both environmental and patient isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings highlight the ICU environment as a reservoir for blaNDM plasmids.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ofid\/ofag663","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:43:34Z","timestamp":1790941414000},"source":"Crossref","is-referenced-by-count":0,"title":["New Delhi Metallo-\u03b2-Lactamase Plasmidomes of Intensive Care Unit Clinical and Environmental Enterobacterales Isolates"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1381-6326","authenticated-orcid":false,"given":"Florencia","family":"Martino","sequence":"first","affiliation":[{"name":"Servicio Antimicrobianos, National Reference Laboratory in Antimicrobial Resistance (NRLAR), National Institute of Infectious Diseases (INEI), ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d , Buenos Aires ,","place":["Argentina"]},{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5568-4804","authenticated-orcid":false,"given":"Denise","family":"De Belder","sequence":"additional","affiliation":[{"name":"UO Centro Nacional de Gen\u00f3mica y Bioinform\u00e1tica, National Center of Genomics and Bioinformatics, ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d , Buenos Aires ,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Monge","sequence":"additional","affiliation":[{"name":"Microbiolog\u00eda, Servicio Laboratorio, Hospital Brit\u00e1nico de Buenos Aires , Buenos Aires ,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2722-1751","authenticated-orcid":false,"given":"Agustina","family":"Forastiero","sequence":"additional","affiliation":[{"name":"Microbiolog\u00eda, Servicio Laboratorio, Hospital Brit\u00e1nico de Buenos Aires , Buenos Aires ,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9623-5909","authenticated-orcid":false,"given":"Molly","family":"Patrick","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Atlanta, Georgia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9247-7388","authenticated-orcid":false,"given":"Kara A","family":"Moser","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Atlanta, Georgia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1183-6746","authenticated-orcid":false,"given":"Alejandra","family":"Corso","sequence":"additional","affiliation":[{"name":"Servicio Antimicrobianos, National Reference Laboratory in Antimicrobial Resistance (NRLAR), National Institute of Infectious Diseases (INEI), ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d , Buenos Aires ,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5840-5869","authenticated-orcid":false,"given":"Fernando","family":"Pasteran","sequence":"additional","affiliation":[{"name":"Servicio Antimicrobianos, National Reference Laboratory in Antimicrobial Resistance (NRLAR), National Institute of Infectious Diseases (INEI), ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d , Buenos Aires ,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0376-8560","authenticated-orcid":false,"given":"Matthew","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7374-9678","authenticated-orcid":false,"given":"Valeria","family":"Fabre","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine , Baltimore, Maryland 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