{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T21:05:13Z","timestamp":1791666313856,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T00:00:00Z","timestamp":1573171200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>With the increasing frequency and impact of Ebola virus disease (EVD) outbreaks illustrated by recent epidemics, good knowledge on extent of viral persistance or RNA detection in body fluids from survivors is urgently needed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Ebola viral RNA shedding was studied with molecular assays in semen (n=1,368), urine (n=1,875), cervico-vaginal fluid (n=549), saliva (n=900), breast milk (n=168) and feces (n=558) from EVD survivors in Guinea (POSTEBOGUI cohort, n=802) at a regular base until 40 months after inclusion.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>27\/277 (9.8%) male survivors tested positive for Ebola RNA in at least one semen sample. The probability of remaining positive for Ebola RNA in semen was estimated at 93.02% and 60.12% after three and six months. Viral RNA in semen was more frequent in patients with eye pain (p=0.036), joint pain (p=0.047), and higher antibody levels to Ebola virus antigens (NP (p=0.001), GP (p=0.05) and VP40 (p=0.05)). Ebola RNA was only rarely detected in other body fluids from EVD survivors\u00a0: saliva (1\/454) urine (2\/593), breast milk (2\/168), cervico-vaginal secretions (0\/273), feces (0\/330). RNA was detected in breast milk one month after delivery but 500 days after discharge of Ebola treatment unit (ETU) in a women who became pregnant seven months after discharge from the ETU.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The frequency and potential long term presence of viral RNA in semen confirm that systematic prevention measures in male survivors are required. Our observation in breast milk suggest that our knowledge on viral reservoir in immune priveledged sites and its impact are still incomplete.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ofid\/ofz482","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T15:20:40Z","timestamp":1573226440000},"source":"Crossref","is-referenced-by-count":38,"title":["A 40 months follow-up of Ebola virus disease survivors in Guinea (Postebogui)\u00a0reveals longterm detection of Ebola viral RNA in semen and breast milk"],"prefix":"10.1093","author":[{"given":"Alpha Kabinet","family":"Keita","sequence":"first","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"},{"name":"Centre de Recherche et de Formation en Infectiologie de Guin\u00e9e, Universit\u00e9 Gamal Abdel Nasser de Conakry, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7022-2643","authenticated-orcid":false,"given":"Nicole","family":"Vidal","sequence":"additional","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdoulaye","family":"Toure","sequence":"additional","affiliation":[{"name":"Centre de Recherche et de Formation en Infectiologie de Guin\u00e9e, Universit\u00e9 Gamal Abdel Nasser de Conakry, Conakry, Guinea"},{"name":"Institut National de Sant\u00e9 Publique, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou Saliou Kalifa","family":"Diallo","sequence":"additional","affiliation":[{"name":"Centre de Recherche et de Formation en Infectiologie de Guin\u00e9e, Universit\u00e9 Gamal Abdel Nasser de Conakry, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u2019Fally","family":"Magassouba","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Conakry University, Projet de Recherche sur les Fi\u00e8vres H\u00e9morragiques en Guin\u00e9e, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Baize","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit of Biology of Emerging Viral Infections - National Reference Center for Viral Hemorrhagic Fevers, Lyon, FranceCentre International de Recherche en Infectiologie, Lyon University - INSERM - ENS Lyon - CNRS, Lyon, France"},{"name":"Laboratoire P4 Inserm-Jean M\u00e9rieux, US003 Inserm, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Mateo","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit of Biology of Emerging Viral Infections - National Reference Center for Viral Hemorrhagic Fevers, Lyon, FranceCentre International de Recherche en Infectiologie, Lyon University - INSERM - ENS Lyon - CNRS, Lyon, France"},{"name":"Laboratoire P4 Inserm-Jean M\u00e9rieux, US003 Inserm, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve","family":"Raoul","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Inserm-Jean M\u00e9rieux, US003 Inserm, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Mely","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Inserm-Jean M\u00e9rieux, US003 Inserm, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Subtil","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Service de Biostatistique, Lyon, France"},{"name":"CNRS UMR 5558 Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive, \u00c9quipe Biostatistique-Sant\u00e9, Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9c\u00e9","family":"Kpamou","sequence":"additional","affiliation":[{"name":"Centre de Recherche et de Formation en Infectiologie de Guin\u00e9e, Universit\u00e9 Gamal Abdel Nasser de Conakry, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamine","family":"Koivogui","sequence":"additional","affiliation":[{"name":"Institut National de Sant\u00e9 Publique, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falaye","family":"Traore","sequence":"additional","affiliation":[{"name":"Institut National de Sant\u00e9 Publique, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou Saliou","family":"Sow","sequence":"additional","affiliation":[{"name":"Centre de Recherche et de Formation en Infectiologie de Guin\u00e9e, Universit\u00e9 Gamal Abdel Nasser de Conakry, Conakry, Guinea"},{"name":"Donka National Hospital, Conakry, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahidjo","family":"Ayouba","sequence":"additional","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Francois","family":"Etard","sequence":"additional","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Delaporte","sequence":"additional","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Peeters","sequence":"additional","affiliation":[{"name":"TransVIHMI, IRD\/INSERM\/Monpellier University, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"POSTEBOGUI Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,11,8]]},"container-title":["Open Forum Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ofid\/advance-article-pdf\/doi\/10.1093\/ofid\/ofz482\/30488293\/ofz482.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ofid\/advance-article-pdf\/doi\/10.1093\/ofid\/ofz482\/30488293\/ofz482.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T15:20:41Z","timestamp":1573226441000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ofid\/advance-article\/doi\/10.1093\/ofid\/ofz482\/5614563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,8]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/ofid\/ofz482","relation":{},"ISSN":["2328-8957"],"issn-type":[{"value":"2328-8957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,8]]},"article-number":"ofz482"}}