{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T06:38:55Z","timestamp":1789799935000,"version":"4.0.1"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>DNA methylation is a promising biomarker for cervical cancer screening. This study aimed to validate the triage performance of cytological DNA methylation for detecting cervical intraepithelial neoplasia of grade 3 or worse (CIN3+) in women with high-risk human papillomavirus (hrHPV) infection from a large prospective cohort undergoing opportunistic screening in China (METHY3).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The triage performance for detecting CIN3+ lesions was compared between HPV16\/18 genotyping, a liquid-based cytology (LBC) test, and the PAX1 and JAM3 methylation (PAX1m\/JAM3m) test according to cervical pathologic outcomes. Among the 4394 women infected with hrHPV, 1105 had definitive cervical histological findings that were analyzed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>For detecting CIN3+, the specificity of HPV16\/18(+), the LBC result of \u2265atypical squamous cells of undetermined significance (ASCUS), and PAX1m\/JAM3m(+) was 66.4%, 23.9%, and 89.6%, respectively, with odds ratios of 4.24 (95% confidence interval [CI], 2.85-6.40), 4.44 (2.27-10.1), and 18.5 (12.1-28.7) (P\u2005&amp;lt;\u2005.001), respectively. PAX1m\/JAM3m(+) had the highest area under the receiver operating characteristic curve (0.790, 95% CI, 0.747-0.832) in the whole cohort and in women of various ages. PAX1m\/JAM3m (+) was detected in all patients with cancer (n\u2005=\u200528). Compared with HPV16\/18 genotyping and the LBC test, PAX1m\/JAM3m testing reduced referrals to colposcopy by 20.64 percentage points and 61.18 percentage points, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>PAX1 m \/JAM3 m testing is highly specific for detecting CIN3+. As a triage biomarker, it is superior to HPV 16\/18 genotyping and LBC testing for women with hrHPV infection.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/oncolo\/oyae324","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T02:35:47Z","timestamp":1732761347000},"source":"Crossref","is-referenced-by-count":13,"title":["Triage performance of DNA methylation for women with high-risk human papillomavirus infection"],"prefix":"10.1093","volume":"30","author":[{"given":"Linghua","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"National Clinical Research Center for Obstetric & Gynecologic Diseases , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"State Key Laboratory for Complex, Severe and Rare Diseases, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"National Clinical Research Center for Obstetric & Gynecologic Diseases , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"State Key Laboratory for Complex, Severe and Rare Diseases, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanwen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, Beijing Origin-Poly Bio-Tec Co., Ltd. , Beijing 102600,","place":["People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghe","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"National Clinical Research Center for Obstetric & Gynecologic Diseases , Beijing 100730,","place":["People\u2019s Republic of China"]},{"name":"State Key Laboratory for Complex, Severe and Rare Diseases, Peking Union Medical College Hospital , Beijing 100730,","place":["People\u2019s Republic of 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