{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:12:34Z","timestamp":1788570754260,"version":"build-2803163510"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2017B020226005"],"award-info":[{"award-number":["2017B020226005"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020771","name":"Young Scientists Fund of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82303178"],"award-info":[{"award-number":["82303178"]}],"id":[{"id":"10.13039\/501100020771","id-type":"DOI","asserted-by":"publisher"}]},{"name":"General Program of the National Natural Science Foundation of China","award":["82572991"],"award-info":[{"award-number":["82572991"]}]},{"name":"Major Scientific Research Issues and Medical Technology Challenges of the China Medicine Education Association","award":["2022KTZ017"],"award-info":[{"award-number":["2022KTZ017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reliable prognostic biomarkers are urgently needed for operable esophageal squamous cell carcinoma (ESCC), as conventional histopathology often fails to detect minimal residual disease and accurately predict patient outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>This study investigated the mutational patterns in ESCC and the prognostic value of shared mutations between tumors and margins (margin-informed tumor, MiT) using next-generation sequencing. We analyzed tumor, margin, and normal tissues from a total of 70 patients with operable ESCC, including 48 from a retrospective cohort and 22 from a prospective cohort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TP53 mutations were more prevalent in tumors than in margins or normal tissues (retrospective, P &amp;lt;.0001; prospective, P = .02), while NOTCH1 mutations were more frequent in normal or margin tissues (retrospective, P = .0002; prospective, P = .07). Positive MiT was observed in 16.7% of the retrospective cohort and 45.5% of the prospective cohort, respectively, with all patients exhibiting over 50% shared mutations experiencing disease progression. Patients with identical TP53 mutations in both tumor and margin tissues (TP53-MiT) exhibited significantly worse survival (retrospective, hazard ratio [HR]\u2009=\u20093.32, 95% confidence interval [CI], 1.11-9.91, P = .03; prospective, HR = 4.35, 95% CI, 0.87-21.63, P = .05). TP53 mutations were associated with tumorigenic pathways and low immune infiltration fraction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings underscore the clinical importance of MiT, particularly TP53-MiT, in postoperative prognosis. Integrating TP53-MiT assessment into postoperative evaluations could refine risk stratification and guide adjuvant management better.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/oncolo\/oyag337","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:43:59Z","timestamp":1787312639000},"source":"Crossref","is-referenced-by-count":0,"title":["Prognostic significance of positive margin-informed tumor status in esophageal squamous cell carcinoma using next-generation sequencing"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8635-6512","authenticated-orcid":false,"given":"Di","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University , Guangzhou, Guangdong 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