{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T20:51:35Z","timestamp":1773607895102,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"vor","delay-in-days":91,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH-PICREID","award":["1U01AI151758"],"award-info":[{"award-number":["1U01AI151758"]}]},{"name":"HHMI-Wellcome Trust International Research Scholar","award":["208710\/Z\/17\/Z"],"award-info":[{"award-number":["208710\/Z\/17\/Z"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dengue virus (DENV) poses a global health threat, affecting millions individuals annually with no specific therapy and limited vaccines. Mosquitoes, mainly Aedes aegypti and Aedes albopictus worldwide, transmit DENV through their saliva during blood meals. In this study, we aimed to understand how Aedes mosquito saliva modulate skin immune responses during DENV infection in individuals living in mosquito-endemic regions. To accomplish this, we dissociated skin cells from Cambodian volunteers and incubated them with salivary gland extract (SGE) from three different mosquito strains: Ae. aegypti USDA strain, Ae. aegypti and Ae. albopictus wild type (WT) in the presence\/absence of DENV. We observed notable alterations in skin immune cell phenotypes subsequent to exposure to Aedes salivary gland extract (SGE). Specifically, exposure lead to an increase in the frequency of macrophages expressing chemokine receptor CCR2, and neutrophils expressing CD69. Additionally, we noted a substantial increase in the percentage of macrophages that became infected with DENV in the presence of Aedes SGE. Differences in cellular responses were observed when Aedes SGE of three distinct mosquito strains were compared. Our findings deepen the understanding of mosquito saliva's role in DENV infection and skin immune responses in individuals regularly exposed to mosquito bites. This study provides insights into skin immune cell dynamics that could guide strategies to mitigate DENV transmission and other arbovirus diseases.<\/jats:p>","DOI":"10.1093\/oxfimm\/iqae003","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T04:36:50Z","timestamp":1711600610000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>In-vitro<\/i>\n                    assessment of cutaneous immune responses to\n                    <i>aedes<\/i>\n                    mosquito salivary gland extract and dengue virus in Cambodian individuals"],"prefix":"10.1093","volume":"5","author":[{"given":"David","family":"Guerrero","sequence":"first","affiliation":[{"name":"Institut Pasteur du Cambodge, Immunology Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sokchea","family":"Lay","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Immunology Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eakpor","family":"Piv","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Malaria Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chansophea","family":"Chhin","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Malaria Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sokkeang","family":"Leng","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Medical and Veterinary Entomology Unit , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratana","family":"Meng","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Immunology Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim Eng","family":"Mam","sequence":"additional","affiliation":[{"name":"Crystal Esthetic Center , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polidy","family":"Pean","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Immunology Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelie","family":"Vantaux","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Malaria Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastien","family":"Boyer","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Medical and Veterinary Entomology Unit , Phnom Penh 12201,","place":["Cambodia"]},{"name":"Institut Pasteur Unit\u00e9 Ecologie et Emergence des Pathog\u00e8nes Transmis par les Arthropodes, , Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doroth\u00e9e","family":"Miss\u00e9","sequence":"additional","affiliation":[{"name":"Univ. Montpellier MIVEGEC, , IRD, CNRS, 34000, Montpellier,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tineke","family":"Cantaert","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Cambodge, Immunology Unit, Pasteur Network , Phnom Penh 12201,","place":["Cambodia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,4,1]]},"reference":[{"key":"2026031516262223900_iqae003-B1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"2026031516262223900_iqae003-B2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41541-023-00658-2","article-title":"Is new dengue vaccine efficacy data a relief or cause for concern?","volume":"8","author":"Thomas","year":"2023","journal-title":"NPJ 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