{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:13:42Z","timestamp":1791335622120,"version":"4.3.1"},"reference-count":82,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001170","name":"Cancer Council WA","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001170","id-type":"DOI","asserted-by":"crossref"}]},{"name":"iCare Dust Diseases Board"},{"name":"UWA Raine Foundation and CCWA"},{"name":"UWA Richard Walter Gibbon Medical Research and Cancer Council WA scholarships"},{"name":"UWA Raine Foundation"},{"name":"Cancer Council WA, WA Department of Health, and iCare Dust Diseases Board"},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"publisher","award":["APP2008104"],"award-info":[{"award-number":["APP2008104"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The National Centre for Asbestos Related Diseases receive funding through the NHMRC Centre of Research Excellence scheme","award":["APP1197652"],"award-info":[{"award-number":["APP1197652"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The success of cancer immunotherapies has highlighted the importance of monitoring the anti-tumour T cell response. Patients with mesothelioma frequently present with a malignant pleural effusion (MPE) that is commonly drained regularly to alleviate symptoms. As MPE contains tumour cells, T cells and cytokines, it provides a unique opportunity to sample immune events at the tumour site. However, there is minimal information on how MPE T cells are distinct from those in the blood, and whether T cell phenotypes unique to each compartment correlate with survival. We characterised T cell populations of matched MPE and blood from 31 mesothelioma patients using flow cytometry and bulk T cell receptor beta (TCR\u03b2) sequencing. MPE CD8+ and CD4+ T cells displayed increased expression of PD-1, TIGIT, LAG-3 and TIM-3 compared to blood, with co-expression of inhibitory receptors greatest on MPE CD8+ T cells with a tissue resident memory T cell phenotype (CD69+CD103+). CD8+ TCR\u03b2 repertoires displayed clonal overlap between MPE and blood, suggesting that a majority of T cells traffic between these compartments. Finally, we show that high expression of PD-1 on circulating CD4+ T cells is an independent prognostic factor for poor survival in this patient group. This work suggests that MPE T cell phenotypes differ from those in circulation, with blood-based T cell subsets more sensitive predictors of outcome in this study.<\/jats:p>","DOI":"10.1093\/oxfimm\/iqaf008","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T12:34:50Z","timestamp":1766406890000},"source":"Crossref","is-referenced-by-count":1,"title":["Comparative analysis of malignant pleural effusion and peripheral blood reveals unique T cell signatures associated with survival in mesothelioma patients"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6458-2482","authenticated-orcid":false,"given":"Nicola","family":"Principe","sequence":"first","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kofi L P","family":"Stevens","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber-Lee","family":"Phung","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"McCoy","sequence":"additional","affiliation":[{"name":"Colorectal Cancer Research Unit, St John of God Subiaco Hospital , Subiaco, WA, 6008,","place":["Australia"]},{"name":"Medical School, University of Western Australia, Crawley , WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Kidman","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Ismail","sequence":"additional","affiliation":[{"name":"Medical School, University of Western Australia, Crawley , WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alistair M","family":"Cook","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abha","family":"Chopra","sequence":"additional","affiliation":[{"name":"IIID Laboratory, Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University , Murdoch, WA, 6150,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Watson","sequence":"additional","affiliation":[{"name":"IIID Laboratory, Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University , Murdoch, WA, 6150,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce W","family":"Robinson","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"Medical School, University of Western Australia, Crawley , WA, 6009,","place":["Australia"]},{"name":"Department of Respiratory Medicine, Sir Charles Gairdner Hospital , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenette","family":"Creaney","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]},{"name":"Department of Respiratory Medicine, Sir Charles Gairdner Hospital , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y C Gary","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Sir Charles Gairdner Hospital , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Waithman","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W Joost","family":"Lesterhuis","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]},{"name":"The Kids Research Institute Australia , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A","family":"Lake","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 6009,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K","family":"Nowak","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"Medical School, University of Western Australia, Crawley , WA, 6009,","place":["Australia"]},{"name":"Department of Medical Oncology, Sir Charles Gairdner Hospital , Perth, WA, 6000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Chee","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, National Centre for Asbestos Related Diseases, University of Western Australia , Perth, WA, 6000,","place":["Australia"]},{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA, 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