{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:18:26Z","timestamp":1783012706755,"version":"3.54.6"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,3,7]],"date-time":"2020-03-07T00:00:00Z","timestamp":1583539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JSPS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"KAKENHI","award":["18K05557"],"award-info":[{"award-number":["18K05557"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Myrosinase (\u03b2-thioglucoside glucohydrolase, enzyme nomenclature, EC 3.2.1.147, TGG) is a highly abundant protein in Arabidopsis guard cells, of which TGG1 and TGG2 function redundantly in abscisic acid (ABA)- and methyl jasmonate-induced stomatal closure. Reactive carbonyl species (RCS) are \u03b1,\u03b2-unsaturated aldehydes and ketones, which function downstream of reactive oxygen species (ROS) production in the ABA signalling pathway in guard cells. Among the RCS, acrolein is the most highly reactive, which is significantly produced in ABA-treated guard cells. To clarify the ABA signal pathway downstream of ROS production, we investigated the responses of tgg mutants (tgg1-3, tgg2-1 and tgg1-3 tgg2-1) to acrolein. Acrolein induced stomatal closure and triggered cytosolic alkalization in wild type (WT), tgg1-3 single mutants and in tgg2-1 single mutants, but not in tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ induced stomatal closure and cytosolic alkalization not only in WT but also in all of the mutants. Acrolein- and Ca2+-induced stomatal closures were inhibited by an intracellular acidifying agent, butyrate, a Ca2+ chelator, ethylene glycol tetraacetic acid (EGTA) and a Ca2+ channel blocker, LaCl3. Acrolein induced cytosolic free calcium concentration ([Ca2+]cyt) elevation in guard cells of WT plants but not in the tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ elicited [Ca2+]cyt elevation in guard cells of WT and tgg1-3 tgg2-1. Our results suggest that TGG1 and TGG2 function redundantly, not between ROS production and RCS production, but downstream of RCS production in the ABA signal pathway in Arabidopsis guard cells.<\/jats:p>","DOI":"10.1093\/pcp\/pcaa024","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T12:18:34Z","timestamp":1583237914000},"page":"967-977","source":"Crossref","is-referenced-by-count":26,"title":["The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells"],"prefix":"10.1093","volume":"61","author":[{"given":"Mohammad Saidur","family":"Rhaman","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Nakamura","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6606-9105","authenticated-orcid":false,"given":"Yoshimasa","family":"Nakamura","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1204-1822","authenticated-orcid":false,"given":"Shintaro","family":"Munemasa","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Murata","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,7]]},"reference":[{"key":"2020051216575745000_pcaa024-B1","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1038\/35082575","article-title":"A defined range of guard cell calcium oscillation parameters encodes stomatal movements","volume":"411","author":"Allen","year":"2001","journal-title":"Nature"},{"key":"2020051216575745000_pcaa024-B2","doi-asserted-by":"crossref","first-page":"2338","DOI":"10.1126\/science.289.5488.2338","article-title":"Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant","volume":"289","author":"Allen","year":"2000","journal-title":"Science"},{"key":"2020051216575745000_pcaa024-B3","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1105\/tpc.010448","article-title":"Hypersensitivity of abscisic acid-induced cytosolic calcium increases in the Arabidopsis farnesyltransferase mutant era1-2","volume":"14","author":"Allen","year":"2002","journal-title":"Plant Cell"},{"key":"2020051216575745000_pcaa024-B4","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/j.1365-313X.2006.02716.x","article-title":"Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense","volume":"46","author":"Barth","year":"2006","journal-title":"Plant J"},{"key":"2020051216575745000_pcaa024-B5","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1007\/BF00195690","article-title":"K+ channels of stomatal guard cells: abscisic-acid-evoked control of the outward rectifier mediated by cytoplasmic pH","volume":"191","author":"Blatt","year":"1993","journal-title":"Planta"},{"key":"2020051216575745000_pcaa024-B6","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1399-3054.1996.tb00497.x","article-title":"The myrosinase-glucosinolate system, its organisation and biochemistry","volume":"97","author":"Bones","year":"1996","journal-title":"Physiol. 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