{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:56:49Z","timestamp":1779890209318,"version":"3.53.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"\u201cProgram on Small Business Innovation Research\u201d from the Project of the Bio-oriented Technology Research Advancement Institution"},{"name":"\u201cProgram on Small Business Innovation Research\u201d from the Project of the Bio-oriented Technology Research Advancement Institution"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sterols are essential components of eukaryotic cell membranes. However, studies on sterol biosynthesis in bryophytes are limited. This study analyzed the sterol profiles in the bryophyte model plant Marchantia polymorpha L. The thalli contained typical phytosterols such as campesterol, sitosterol and stigmasterol. BLASTX analysis of the M. polymorpha genome against the Arabidopsis thaliana sterol biosynthetic genes confirmed the presence of all the enzymes responsible for sterol biosynthesis in M. polymorpha. We further focused on characterizing two genes, MpDWF5A and MpDWF5B, which showed high homology with A. thaliana DWF5, encoding \u03945,7-sterol \u03947-reductase (C7R). Functional analysis using a yeast expression system revealed that MpDWF5A converted 7-dehydrocholesterol to cholesterol, indicating that MpDWF5A is a C7R. Mpdwf5a-knockout (Mpdwf5a-ko) lines were constructed using CRISPR\/Cas9-mediated genome editing. Gas chromatography\u2013mass spectrometry analysis of Mpdwf5a-ko revealed that phytosterols such as campesterol, sitosterol and stigmasterol disappeared, and instead, the corresponding \u03947-type sterols accumulated. The thalli of Mpdwf5a-ko grew smaller than those of the wild type, and excessive formation of apical meristem in the thalli was observed. In addition, the gemma cups of the Mpdwf5a-ko were incomplete, and only a limited number of gemma formations were observed. Treatment with 1\u2009\u00b5M of castasterone or 6-deoxocastasterone, a bioactive brassinosteroid (BR), partly restored some of these abnormal phenotypes, but far from complete recovery. These results indicate that MpDWF5A is essential for the normal growth and development of M. polymorpha and suggest that the dwarfism caused by the Mpdwf5a-ko defect is due to the deficiency of typical phytosterols and, in part, a BR-like compound derived from phytosterols.<\/jats:p>","DOI":"10.1093\/pcp\/pcad043","type":"journal-article","created":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T16:14:39Z","timestamp":1683994479000},"page":"826-838","source":"Crossref","is-referenced-by-count":7,"title":["<i>MpDWF5A<\/i>-Encoded Sterol \u03947-Reductase Is Essential for the Normal Growth and Development of <i>Marchantia polymorpha<\/i>"],"prefix":"10.1093","volume":"64","author":[{"given":"Miki","family":"Hatada","sequence":"first","affiliation":[{"name":"Graduate School of Agricultural Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, 657-8501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, 657-8501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moeko","family":"Yamagishi","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, 657-8501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0504-8196","authenticated-orcid":false,"given":"Kimitsune","family":"Ishizaki","sequence":"additional","affiliation":[{"name":"Graduate School of Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, 657-8501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4321-0644","authenticated-orcid":false,"given":"Masaharu","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, 657-8501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,5,11]]},"reference":[{"key":"2023071804422079600_R1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.cell.2017.09.030","article-title":"Insights into land plant evolution garnered from the Marchantia polymorpha genome","volume":"171","author":"Bowman","year":"2017","journal-title":"Cell"},{"key":"2023071804422079600_R2","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1016\/j.cub.2020.02.086","article-title":"Multiple metabolic innovations and losses are associated with major transitions in land plant evolution","volume":"30","author":"Cannell","year":"2020","journal-title":"Curr. 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