{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:27:53Z","timestamp":1776259673799,"version":"3.50.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP19K06731"],"award-info":[{"award-number":["JP19K06731"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP19K06731"],"award-info":[{"award-number":["JP19K06731"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Folate, also known as vitamin B9, is an essential cofactor for a variety of enzymes and plays a crucial role in many biological processes. We previously reported that plastidial folate prevents starch biosynthesis triggered by the influx of sugar into non-starch-accumulating plastids, such as etioplasts, and chloroplasts under darkness; hence the loss of plastidial folate induces the accumulation of starch in plastids. To understand the molecular mechanism underlying this phenomenon, we screened our in-house chemical library and searched their derivatives to identify chemicals capable of inducing starch accumulation in etioplasts. The results revealed four chemicals, compounds #120 and #375 and their derivatives, compounds #120d and #375d, respectively. The derivative compounds induced starch accumulation in etioplasts and suppressed hypocotyl elongation in dark-grown Arabidopsis seedlings. They also inhibited the post-germinative growth of seedlings under illumination. All four chemicals contained the sulfonamide group as a consensus structure. The sulfonamide group is also found in sulfa drugs, which exhibit antifolate activity, and in sulfonylurea herbicides. Further analyses revealed that compound #375d induces starch accumulation by inhibiting folate biosynthesis. By contrast, compound #120d neither inhibited folate biosynthesis nor exhibited the herbicide activity. Protein and metabolite analyses suggest that compound #120d abrogates folate-dependent inhibition of starch accumulation in etioplasts by enhancing starch biosynthesis.<\/jats:p>","DOI":"10.1093\/pcp\/pcad116","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T15:13:35Z","timestamp":1696605215000},"page":"1551-1562","source":"Crossref","is-referenced-by-count":1,"title":["Identification of\u00a0Chemicals That Abrogate Folate-Dependent Inhibition of\u00a0Starch Accumulation in\u00a0Non-Photosynthetic Plastids of\u00a0<i>Arabidopsis<\/i>"],"prefix":"10.1093","volume":"64","author":[{"given":"Yoshihiro","family":"Kawada","sequence":"first","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriko","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Katsuragi","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aya","family":"Imamura-Jinda","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takatsugu","family":"Hirokawa","sequence":"additional","affiliation":[{"name":"Transborder Medical Research Center, Tsukuba University , 1-1-1 Tenmondai, Tsukuba, Ibaragi, 305-8577 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamio","family":"Mizukami","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"},{"name":"Frontier Pharma , 1281-8 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2207-257X","authenticated-orcid":false,"given":"Makoto","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Nagahama Institute of Bioscience and Technology , 1266 Tamura, Nagahama, Shiga, 526-0829 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,10,6]]},"reference":[{"key":"2023122117542050600_R1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1093\/jxb\/ert483","article-title":"Present and\u00a0future of\u00a0folate biofortification of\u00a0crop plants","volume":"65","author":"Blancquaert","year":"2014","journal-title":"J. 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