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This study focuses on APX-R from Chlamydomonas reinhardtii, namely, ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the photosystem I primary electron donor, P700, upon sudden light increase and a slower re-reduction rate compared to the wild type, pointing to a limitation of plastocyanin. Spectroscopic, proteomic and immunoblot analyses confirmed that the phenotype was a result of lower levels of plastocyanin in the apx2 mutants. The redox state of P700 did not differ between wild type and apx2 mutants when the loss of function in plastocyanin was nutritionally complemented by growing apx2 mutants under copper deficiency. In this case, cytochrome c6 functionally replaces plastocyanin, confirming that lower levels of plastocyanin were the primary defect caused by the absence of APX2. Overall, the results presented here shed light on an unexpected regulation of plastocyanin level under copper-replete conditions, induced by APX2 in Chlamydomonas.<\/jats:p>","DOI":"10.1093\/pcp\/pcae019","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T11:29:44Z","timestamp":1712662184000},"page":"644-656","source":"Crossref","is-referenced-by-count":6,"title":["APX2 Is an Ascorbate Peroxidase\u2013Related Protein that Regulates the Levels of Plastocyanin in <i>Chlamydomonas<\/i>"],"prefix":"10.1093","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-313X","authenticated-orcid":false,"given":"Anna","family":"Caccamo","sequence":"first","affiliation":[{"name":"Genetics and Physiology of Microalgae, InBios\/Phytosystems Research Unit, University of Li\u00e8ge , Chemin de la vall\u00e9e 4, Li\u00e8ge 4000, Belgium"},{"name":"VIB-VUB Center for Structural Biology , Pleinlaan 2, Brussels 1050, Belgium"},{"name":"Brussels Center for Redox Biology , Pleinlaan 2, Brussels 1050, Belgium"},{"name":"Structural Biology Brussels, Vrije Universiteit Brussel , Pleinlaan 2, Brussels 1050, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3935-7499","authenticated-orcid":false,"given":"F\u00e9lix","family":"Vega de Luna","sequence":"additional","affiliation":[{"name":"Genetics and Physiology of Microalgae, InBios\/Phytosystems Research Unit, University of Li\u00e8ge , Chemin de la vall\u00e9e 4, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5977-3532","authenticated-orcid":false,"given":"Agnieszka E","family":"Misztak","sequence":"additional","affiliation":[{"name":"Genetics and Physiology of Microalgae, InBios\/Phytosystems Research Unit, University of Li\u00e8ge , Chemin de la vall\u00e9e 4, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5449-1541","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Pyr dit Ruys","sequence":"additional","affiliation":[{"name":"de Duve Institute and MASSPROT platform, UCLouvain , Avenue Hippocrate 74, Brussels 1200, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7648-8282","authenticated-orcid":false,"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"de Duve Institute and MASSPROT platform, UCLouvain , Avenue Hippocrate 74, Brussels 1200, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9799-0546","authenticated-orcid":false,"given":"Pierre","family":"Cardol","sequence":"additional","affiliation":[{"name":"Genetics and Physiology of Microalgae, InBios\/Phytosystems Research Unit, University of Li\u00e8ge , Chemin de la vall\u00e9e 4, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2128-8264","authenticated-orcid":false,"given":"Joris","family":"Messens","sequence":"additional","affiliation":[{"name":"VIB-VUB Center for Structural Biology , Pleinlaan 2, Brussels 1050, Belgium"},{"name":"Brussels Center for Redox Biology , Pleinlaan 2, Brussels 1050, Belgium"},{"name":"Structural Biology Brussels, Vrije Universiteit Brussel , Pleinlaan 2, Brussels 1050, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5016-9547","authenticated-orcid":false,"given":"Claire","family":"Remacle","sequence":"additional","affiliation":[{"name":"Genetics and Physiology of Microalgae, InBios\/Phytosystems Research Unit, University of Li\u00e8ge , Chemin de la vall\u00e9e 4, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,2]]},"reference":[{"key":"2024051510362621000_R1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s00425-008-0869-z","article-title":"Contribution of plastocyanin isoforms to photosynthesis and copper homeostasis in Arabidopsis thaliana grown at different copper regimes","volume":"229","author":"Abdel-Ghany","year":"2009","journal-title":"Planta"},{"key":"2024051510362621000_R2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1146\/annurev.arplant.50.1.601","article-title":"THE WATER-WATER CYCLE IN CHLOROPLASTS: scavenging of active oxygens and dissipation of excess photons","volume":"50","author":"Asada","year":"1999","journal-title":"Ann. 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