{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T02:57:10Z","timestamp":1755226630595,"version":"3.43.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Graduate School of Environmental and Life Science, Okayama University"},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18F18391 18H02169 23K05025"],"award-info":[{"award-number":["18F18391 18H02169 23K05025"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Graduate School of Environmental and Life Science, Okayama University"},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18F18391 18H02169 23K05025"],"award-info":[{"award-number":["18F18391 18H02169 23K05025"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Guard cells surrounding the stomata play a crucial role in regulating the entrance of hazardous gases such as SO2 into leaves. Stomatal closure could be a plant response to mitigate SO2 damage, although the mechanism for SO2-induced closure remains controversial. Proposed mediators for SO2-induced stomatal closure include phytohormones, reactive oxygen species, gasotransmitters, and cytosolic acidification. In this study, we investigated the mechanism of stomatal closure in Arabidopsis in response to SO2. Despite an increment in auxin and jasmonates after SO2 exposure, the addition of auxin did not cause stomatal closure and jasmonate-insensitive mutants exhibited SO2-induced stomatal closure suggesting auxin and jasmonates are not mediators leading to the closure. In addition, supplementation of scavenging reagents for reactive oxygen species and gasotransmitters did not inhibit SO2-induced closure. Instead, we found that cytosolic acidification is a credible mechanism for SO2-induced stomatal closure in Arabidopsis. CLCa mutants coding H+\/nitrate antiporter, involved in cytosolic pH homeostasis, showed less sensitive stomatal phenotype against SO2. These results suggest that cytosolic pH homeostasis plays a tenable role in SO2 response in guard cells.<\/jats:p>","DOI":"10.1093\/pcp\/pcaf042","type":"journal-article","created":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T07:50:16Z","timestamp":1746345016000},"page":"1076-1085","source":"Crossref","is-referenced-by-count":0,"title":["Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate\/proton antiporter <i>AtCLCa<\/i> mutants"],"prefix":"10.1093","volume":"66","author":[{"given":"Lia","family":"Ooi","sequence":"first","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University , 2-20-1 Chuo, Kurashiki, Okayama 710-0046,","place":["Japan"]},{"name":"Plant & Microbial Research Unit, Research, Technology & Value Creation Division, Nagase Viita Co., Ltd. , 675-1 Fujisaki, Naka-ku, Okayama 702-8006,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takakazu","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University , 2-20-1 Chuo, Kurashiki, Okayama 710-0046,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-0779","authenticated-orcid":false,"given":"Izumi C","family":"Mori","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University , 2-20-1 Chuo, Kurashiki, Okayama 710-0046,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"2025081302220346400_R1","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1080\/00021369.1973.10860693","article-title":"The photo-oxidation of epinephrine by spinach chloroplasts and its inhibition by superoxide dismutase: evidence for the formation of superoxide radicals in chloroplasts","volume":"37","author":"Asada","year":"1973","journal-title":"Agr. 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