{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:06:08Z","timestamp":1785240368612,"version":"3.55.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Jiangsu Forestry Science and Technology Innovation and Extension Project","award":["LYKJ [2024]01"],"award-info":[{"award-number":["LYKJ [2024]01"]}]},{"name":"Jiangsu Province Young Science and Technology Talent Support Project","award":["JSTJ-2024-655"],"award-info":[{"award-number":["JSTJ-2024-655"]}]},{"name":"Suqian Sci&Tech Program","award":["K202329"],"award-info":[{"award-number":["K202329"]}]},{"name":"Suqian University Talent Introduction Scientific Research Initiation Fund Project","award":["2023XRC011"],"award-info":[{"award-number":["2023XRC011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ginkgo biloba seeds contain toxic 4\u2032-O-methylpyridoxine (MPN) and 4\u2032-O-methylpyridoxine-5\u2032-glucoside (MPNG), but their biosynthetic genes are poorly understood. To identify these genes, we performed multistage transcriptome sequencing, differentially expressed gene screening, weighted gene co-expression network analysis (WGCNA), and enrichment analysis during seed development. MPNG accumulation predominated in mature seeds, correlating with gene expression dynamics. We identified 25 O-methyltransferase (OMT) and 40 uridine diphosphate (UDP)-glucosyltransferase (UGT) candidates. Among these, 7 OMTs (e.g. Gb_35680 and Gb_01186) and 8 UGTs (e.g. Gb_34745 and Gb_37494) were significantly upregulated during maturation, confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). WGCNA revealed four gene modules strongly correlated with MPN\/MPNG accumulation, enriched in methylation, glycosylation, and secondary metabolite biosynthesis. This study integrates dynamic transcriptome and coexpression analyses to pinpoint key regulatory nodes in ginkgotoxin biosynthesis, providing novel genetic targets for developing low-toxicity cultivars through molecular breeding.<\/jats:p>","DOI":"10.1093\/pcp\/pcag046","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:47:10Z","timestamp":1775821630000},"page":"1165-1180","source":"Crossref","is-referenced-by-count":0,"title":["Mining of key O-methyltransferase genes and UDP-glucosyltransferase genes for ginkgotoxin and ginkgotoxin-5\u2032-glucoside synthesis based on transcriptome sequencing analysis"],"prefix":"10.1093","volume":"67","author":[{"given":"Manman","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory for Development and Utilization of Forest Food Resources, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]},{"name":"Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Development and Utilization of Forest Food Resources, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]},{"name":"Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]},{"name":"Department of Biological Engineering, School of Biology and Materials Engineering, Suqian University , No. 399 Huanghe Road, Sucheng District, Suqian, Jiangsu Province, 223800 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Development and Utilization of Forest Food Resources, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]},{"name":"Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Yu","sequence":"additional","affiliation":[{"name":"Suining County Runqi Investment Co., Ltd , Weijing Building A, South Central Avenue, Suining County, Xuzhou, Jiangsu Province 210037 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuliang","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Development and Utilization of Forest Food Resources, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3868-567X","authenticated-orcid":false,"given":"Erzheng","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Development and Utilization of Forest Food Resources, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]},{"name":"Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University , No. 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu Province, 210037 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"key":"2026072807515843100_ref1","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1055\/s-2006-957967","article-title":"Occurrence of neurotoxic 4\u2032-O-methylpyridoxine in Ginkgo biloba leaves, Ginkgo medications and Japanese Ginkgo food","volume":"62","author":"Arenz","year":"1996","journal-title":"Planta Med."},{"key":"2026072807515843100_ref2","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1093\/chromsci\/bmw167","article-title":"Proteomics: technologies and their applications","volume":"55","author":"Aslam","year":"2017","journal-title":"J. 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