{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T11:12:02Z","timestamp":1788606722089,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP18K05439"],"award-info":[{"award-number":["JP18K05439"]}]},{"name":"JSPS KAKENHI","award":["JP21K05382"],"award-info":[{"award-number":["JP21K05382"]}]},{"name":"JSPS KAKENHI","award":["JP26K08631"],"award-info":[{"award-number":["JP26K08631"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Riboflavin (vitamin B\u2082; RF) and its derivatives flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are indispensable cofactors for redox reactions in plants. While higher plants possess a conserved pathway for de novo RF biosynthesis, how flavins are transported and spatially distributed between tissues remains unresolved. In particular, the molecular identity of plasma membrane (PM) transporters involved in flavin transport in plants remains largely unknown. Here, we identify Arabidopsis purine permease 5 (AtPUP5) as a PM\u2013localized protein associated with RF transport and its distribution in plants. Using an RF-auxotrophic yeast mutant, we show that AtPUP5 enhances intracellular accumulation of RF and, to a lesser extent, FMN, whereas FAD accumulation is inefficient under the conditions tested. In planta, AtPUP5 overexpression increases RF accumulation following external application. In contrast, loss-of-function mutants do not display defects in bulk RF uptake at the whole-plant level, indicating that AtPUP5 is not essential for global RF acquisition. Notably, AtPUP5 deficiency results in RF overaccumulation in reproductive organs, including inflorescences, siliques, and seeds, irrespective of external RF supply. This organ-specific phenotype is fully suppressed by genetic complementation and coincides spatially with strong AtPUP5 promoter activity in reproductive tissues. These findings suggest that AtPUP5 contributes to the regulation of localized RF distribution in reproductive tissues. Together, our study provides a molecular framework for PM\u2013mediated regulation of RF distribution in plants and highlights spatial regulation as an important component of flavin homeostasis.<\/jats:p>","DOI":"10.1093\/pcp\/pcag061","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:20:23Z","timestamp":1778498423000},"page":"1399-1413","source":"Crossref","is-referenced-by-count":0,"title":["Purine permease 5 contributes to riboflavin distribution in\n                    <i>Arabidopsis<\/i>\n                    reproductive organs"],"prefix":"10.1093","volume":"67","author":[{"given":"Rui","family":"Shibata","sequence":"first","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikari","family":"Kuwata","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuto","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madoka","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-2922","authenticated-orcid":false,"given":"Takahiro","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]},{"name":"Department of Life Sciences, Faculty of Life and Environmental Sciences, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]},{"name":"Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1816-7391","authenticated-orcid":false,"given":"Takahisa","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]},{"name":"Department of Life Sciences, Faculty of Life and Environmental Sciences, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]},{"name":"Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University , 1060 Nishikawatsu, Matsue, Shimane 690-8504 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"2026090506314449600_ref1","doi-asserted-by":"publisher","first-page":"pdb-top077628","DOI":"10.1101\/pdb.top077628","article-title":"Classical genetics with Saccharomyces cerevisiae","volume":"2016","author":"Amberg","year":"2016","journal-title":"Cold Spring Harb. 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