{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T17:08:18Z","timestamp":1789232898329,"version":"build-2803163510"},"reference-count":131,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"JST SPRING","award":["JPMJSP2119"],"award-info":[{"award-number":["JPMJSP2119"]}]},{"name":"EXEX (formerly DX) Doctoral Fellowship, Hokkaido University","award":["8120060776"],"award-info":[{"award-number":["8120060776"]}]},{"name":"EXEX (formerly DX) Doctoral Fellowship, Hokkaido University","award":["8D01230776"],"award-info":[{"award-number":["8D01230776"]}]},{"name":"JSPS KAKENHI","award":["JP21H02516"],"award-info":[{"award-number":["JP21H02516"]}]},{"name":"JSPS KAKENHI","award":["JP21K19247"],"award-info":[{"award-number":["JP21K19247"]}]},{"name":"JSPS KAKENHI","award":["JP25K02290"],"award-info":[{"award-number":["JP25K02290"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cell\u2013cell communication plays a crucial role during plant growth and development. While plasmodesmata (PD) are well known in this communication, their biogenesis and regulation remain poorly understood. In this study, we demonstrate that xyloglucan endotransglycosylases (XETs), enzymes involved in the transglycosylation of xyloglucan chains, impact not only cellular growth but also PD development in the moss Physcomitrium patens (P. patens). Knockout mutant lines of two XET isoforms, named Ppxth5, Ppxth6, and Ppxth5,6 dko, showed growth reductions in both protonemal and gametophytic stages. Subsequent analyses of these mutants indicated a decrease in PD density, abnormal clusters of xyloglucan and callose, and their colocalization, highlighting their potential involvement in PD formation. Further evaluation of these alterations using Dendra2 photoconvertible protein showed impaired intercellular communication, indicating reduced intercellular transport compared to wild-type. For PD structural changes, these mutants show higher ratios of incomplete simple PD and complex PD, highlighting the critical role of xyloglucan. Specifically, proper xyloglucan distribution, which is regulated by XET activities, is required for maintenance of PD density and structure, and for intercellular transport, possibly interacting with callose during cell plate formation.<\/jats:p>","DOI":"10.1093\/pcp\/pcag113","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:15:16Z","timestamp":1786634116000},"source":"Crossref","is-referenced-by-count":0,"title":["PpXTH5 and PpXTH6, xyloglucan endotransglycosylases, regulate plasmodesmata density and structure in the moss\n                    <i>Physcomitrium patens<\/i>"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3405-1706","authenticated-orcid":false,"given":"Phu Thanh","family":"Ngo","sequence":"first","affiliation":[{"name":"Division of Life Science, Graduate School of Life Science, Hokkaido University , Kita 10, Nishi 8, Kita-ku, Sapporo 060-0810 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Pascal Beier","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science, Hokkaido University , Kita10 Nishi 8, Kita-ku, Sapporo 060-0810 ,","place":["Japan"]},{"name":"Institute for the Advancement of Higher Education, Hokkaido University , Kita 17, Nishi 8, Kita-ku, Sapporo 060-0817 ,","place":["Japan"]},{"name":"Soil and Plant Multi-Dynamics Research Unit, Fukushima Institute for Research, Education and Innovation , 6-1 Yazawa-machi, Gongendo, Namie Town, Futaba County, Fukushima 960-1296 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Louisville , 214 W. Barbee Street Walk, Louisville, KY 40208 ,","place":["USA"]},{"name":"Philips Institute for Oral Health Research, Virginia Commonwealth University , 521 North 11th Street Box 980566, Richmond, VA 23298-0566 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P","family":"Running","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Louisville , 214 W. Barbee Street Walk, Louisville, KY 40208 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1434-8705","authenticated-orcid":false,"given":"Tomomichi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science, Hokkaido University , Kita10 Nishi 8, Kita-ku, Sapporo 060-0810 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026091212471002400_ref1","doi-asserted-by":"publisher","first-page":"4538","DOI":"10.1038\/s41467-018-06820-y","article-title":"Interactions between callose and cellulose revealed through the analysis of biopolymer mixtures","volume":"9","author":"Abou-Saleh","year":"2018","journal-title":"Nat. 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Xyloglucan-cellulose interactions in vitro and in vivo","volume":"83","author":"Hayashi","year":"1987","journal-title":"Plant Physiol."},{"key":"2026091212471002400_ref43","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/BF01282070","article-title":"Endoplasmic reticulum in the formation of the cell plate and plasmodesmata","volume":"111","author":"Hepler","year":"1982","journal-title":"Protoplasma."},{"key":"2026091212471002400_ref44","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1094\/MPMI-23-5-0549","article-title":"The role of callose deposition along plasmodesmata in nematode feeding sites","volume":"23","author":"Hofmann","year":"2010","journal-title":"MPMI."},{"key":"2026091212471002400_ref45","first-page":"755","article-title":"A cell plate\u2013specific callose synthase and its interaction with phragmoplastin","volume":"13","author":"Hong","year":"2001","journal-title":"Plant Cell"},{"key":"2026091212471002400_ref46","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1046\/j.1365-313x.2000.00658.x","article-title":"Movement of plant viruses is delayed in a \u03b2-1,3-glucanase-deficient mutant showing a reduced plasmodesmatal size exclusion limit and enhanced callose deposition","volume":"21","author":"Iglesias","year":"2000","journal-title":"Plant J."},{"key":"2026091212471002400_ref47","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/s10265-021-01361-w","article-title":"Reconsidering the function of the xyloglucan endotransglucosylase\/hydrolase family","volume":"135","author":"Ishida","year":"2022","journal-title":"J. Plant Res."},{"key":"2026091212471002400_ref48","doi-asserted-by":"publisher","first-page":"2503","DOI":"10.1105\/tpc.016097","article-title":"An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation","volume":"15","author":"Jacobs","year":"2003","journal-title":"Plant Cell"},{"key":"2026091212471002400_ref49","doi-asserted-by":"publisher","first-page":"eadr8298","DOI":"10.1126\/sciadv.adr8298","article-title":"Abscisic acid signaling regulates primary plasmodesmata density for plant cell-to-cell communication","volume":"11","author":"Jinno","year":"2025","journal-title":"Sci. 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A."},{"key":"2026091212471002400_ref55","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s10265-013-0547-5","article-title":"Quantitative imaging of directional transport through plasmodesmata in moss protonemata via single-cell photoconversion of Dendra2","volume":"126","author":"Kitagawa","year":"2013","journal-title":"J. Plant Res."},{"key":"2026091212471002400_ref56","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1093\/pcp\/pcy249","article-title":"Abscisic acid acts as a regulator of molecular trafficking through plasmodesmata in the moss Physcomitrella patens","volume":"60","author":"Kitagawa","year":"2019","journal-title":"Plant Cell Physiol."},{"key":"2026091212471002400_ref57","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"Kremer","year":"1996","journal-title":"J. Struct. 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