{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:07:40Z","timestamp":1788890860477,"version":"build-2803163510"},"reference-count":45,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32573015"],"award-info":[{"award-number":["32573015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360743"],"award-info":[{"award-number":["32360743"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072559"],"award-info":[{"award-number":["32072559"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lateral bud outgrowth critically determines tomato plant architecture, canopy structure, photosynthate distribution, and fruit yield. SQUAMOSA promoter binding protein-like (SPL) transcription factors regulate multiple developmental processes; however, the role of Solanum lycopersicum SPL10 (SlSPL10) in controlling lateral bud outgrowth remains unknown. In this study, we used the tomato cultivar Micro-Tom (wild type), SlSPL10 knockout mutants (slspl10#1 and slspl10#2), and overexpression lines (OE-SlSPL10#6 and OE-SlSPL10#7) to assess agronomic traits and phytohormone levels. The slspl10 mutants exhibited dwarfism, an increased lateral bud number, and elongated buds, whereas the OE-SlSPL10 lines suppressed bud outgrowth. Phytohormone and sucrose analyses revealed that the slspl10 mutants displayed elevated levels of zeatin (ZT), gibberellin 3 (GA3), and sucrose, but reduced levels of indole-3-acetic acid (IAA), abscisic acid (ABA), and salicylic acid (SA), whereas the OE-SlSPL10 lines showed the opposite trends. To dissect the underlying molecular mechanism, we demonstrated that SlSPL10 directly binds to GTAC motifs in the promoters of Solanum lycopersicum AUXIN RESPONSE FACTOR 10a (SlARF10a) and Solanum lycopersicum ABA INSENSITIVE 3 (SlABI3) using yeast one-hybrid assays, dual-luciferase reporter assays, and chromatin immunoprecipitation-PCR, thereby transactivating both target genes. Virus-induced gene silencing (VIGS) of SlARF10a or SlABI3 phenocopied the slspl10 mutants, showing an increased lateral bud number and elongation. Hormone measurements in the VIGS lines further indicated that silencing SlARF10a specifically reduced the IAA content, whereas silencing SlABI3 specifically reduced the ABA content; both lines also exhibited increased ZT, GA3, and sucrose, and decreased SA. Collectively, these results demonstrate that SlSPL10 directly targets SlARF10a and SlABI3 to modulate phytohormone and sucrose homeostasis, thereby precisely inhibiting lateral bud outgrowth in tomato.<\/jats:p>","DOI":"10.1093\/pcp\/pcag117","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:56:29Z","timestamp":1786017389000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Sl<\/i>\n                    SPL10, a transcription factor that suppresses lateral bud outgrowth in tomato"],"prefix":"10.1093","author":[{"given":"Di","family":"Yang","sequence":"first","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University , 1 Yinmen Village, Anning District, Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengjing","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University , 1 Yinmen Village, Anning District, Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yandong","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shanxi Agricultural University , 81 Longcheng Street, Xiaodian District, Taiyuan City 030031, Shanxi Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University , 1 Yinmen Village, Anning District, Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-2145","authenticated-orcid":false,"given":"Weibiao","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University , 1 Yinmen Village, Anning District, Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"2026090813222399600_ref1","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1105\/tpc.106.048934","article-title":"Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds","volume":"19","author":"Aguilar-Mart\u00ednez","year":"2007","journal-title":"Plant Cell."},{"key":"2026090813222399600_ref2","doi-asserted-by":"publisher","first-page":"20242","DOI":"10.1073\/pnas.1111902108","article-title":"Strigolactone signaling is required for auxin-dependent stimulation of secondary growth in plants","volume":"108","author":"Agusti","year":"2011","journal-title":"Proc. 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