{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:00:00Z","timestamp":1790211600608,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science KAKENHI","award":["22H02647"],"award-info":[{"award-number":["22H02647"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Japan Society for the Promotion of Science KAKENHI","award":["23K27197"],"award-info":[{"award-number":["23K27197"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Japan Society for the Promotion of Science KAKENHI","award":["24H022501"],"award-info":[{"award-number":["24H022501"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Japan Society for the Promotion of Science KAKENHI","award":["19K06728"],"award-info":[{"award-number":["19K06728"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Japan Society for the Promotion of Science KAKENHI","award":["23K05806"],"award-info":[{"award-number":["23K05806"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"crossref"}]},{"name":"ACRO Research grant of Teikyo university","award":["TeTe20-01"],"award-info":[{"award-number":["TeTe20-01"]}],"id":[{"id":"https:\/\/ror.org\/01gaw2478","id-type":"ROR","asserted-by":"crossref"}]},{"name":"R4\u20136 Research and Development Grant of G-7 Scholarship Foundation"},{"name":"35th Botanical Research Grant of ICHIMURA Foundation for New Technology"},{"name":"Japan Science and Technology Agency SPRING","award":["JPMJSP2124"],"award-info":[{"award-number":["JPMJSP2124"]}],"id":[{"id":"https:\/\/ror.org\/00097mb19","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Teikyo University","id":[{"id":"https:\/\/ror.org\/01gaw2478","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>When vascular tissues are damaged, plants induce site-specific formation of vascular stem cells that proliferate and differentiate into xylem and phloem, restoring vascular continuity. In addition, reactive oxygen species (ROS) are rapidly generated at wound sites in response to damage. However, the role of ROS during vascular regeneration remains largely unknown. Here, we investigated the role of ROS using an Arabidopsis inflorescence stem incision model and the vascular cell induction culture system (VISUAL). During tissue reunion of incised inflorescence stems, scavenging ROS with potassium iodide (KI) suppressed the proliferation of cambial cell layers. Consistently, the expression of cambium- and xylem-related genes, including TDR\/PXY and VND7, was significantly reduced by KI treatment. Furthermore, mutation in respiratory burst oxidase homolog D (RBOHD), which encodes an ROS-producing enzyme, suppressed the proliferation of cambial cell layers. In VISUAL, KI treatment inhibited ectopic xylem formation and reduced the expression of vascular stem cell-related genes. Temporal analyses further indicated that ROS functions during the early phase of induction, as the expression of dedifferentiation-related genes such as WAK1 and ACS6 decreased under KI treatment. Notably, the expression of Arabidopsis NAC domain-containing protein 071 (ANAC071) and ANAC096 was not suppressed by KI in incised stems, and estradiol-induced expression of ANAC071 failed to rescue vascular differentiation in VISUAL under KI treatment. These findings demonstrated that ROS act prior to ANAC function during the early phase of vascular regeneration.<\/jats:p>","DOI":"10.1093\/pcp\/pcag120","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:50:34Z","timestamp":1787313034000},"source":"Crossref","is-referenced-by-count":0,"title":["ROS function during the early phase of vascular regeneration in\n                    <i>Arabidopsis<\/i>"],"prefix":"10.1093","author":[{"given":"Yusuke","family":"Ohba","sequence":"first","affiliation":[{"name":"Graduate school of Life and Environmental Science, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572 ,","place":["Japan"]},{"name":"Environment and Biotechnology course, Department of Integrated Science and Engineering, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuyi","family":"Li","sequence":"additional","affiliation":[{"name":"Graduate school of Life and Environmental Science, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0473-1460","authenticated-orcid":false,"given":"Keita","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1118-8662","authenticated-orcid":false,"given":"Yuki","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Graduate School of Science, The University of Osaka , 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8854-0375","authenticated-orcid":false,"given":"Kazuyuki","family":"Kuchitsu","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science, Tokyo University of Science , 2641 Yamazaki, Noda, Chiba 278-8510 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Satoh","sequence":"additional","affiliation":[{"name":"Institute of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2525-9476","authenticated-orcid":false,"given":"Hiroaki","family":"Iwai","sequence":"additional","affiliation":[{"name":"Institute of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572 ,","place":["Japan"]},{"name":"Department of Biology, School of Biological Sciences, Tokai University , 5-1-1-1 Minamisawa, Minami-ku, Sapporo, Hokkaido 005-8601 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1828-0966","authenticated-orcid":false,"given":"Masashi","family":"Asahina","sequence":"additional","affiliation":[{"name":"Environment and Biotechnology course, Department of Integrated Science and Engineering, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]},{"name":"Department of Biosciences, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]},{"name":"Advanced Instrumental Analysis Center, Teikyo University , 1-1 Toyosatodai, Utsunomiya, Tochigi 320-8551 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026092320023144200_ref1","doi-asserted-by":"publisher","first-page":"16128","DOI":"10.1073\/pnas.1110443108","article-title":"Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue Reunion in Arabidopsis","volume":"108","author":"Asahina","year":"2011","journal-title":"Proc. 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