{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:41:13Z","timestamp":1792590073466,"version":"4.5.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Plasma membrane transporters play an essential role in plant development and all physiological processes. Their activity is tightly controlled by a variety of mechanisms, including the rate of transporter endocytosis. There is evidence that the local membrane environment, in particular the sterol content, influences transporter endocytosis. Here, we analyzed the sucrose transporters StSUT1 (potato) and AtSUC2 (Arabidopsis) transiently expressed in Nicotiana benthamiana epidermal cells. Using live-cell imaging of fluorescent protein fusions combined with extracellular quenching by Trypan Blue, we quantitatively distinguished plasma membrane\u2013localized from internal transporter pools. Pharmacological perturbation of sterols and sphingolipids with methyl-\u03b2-cyclodextrin, fenpropimorph and myriocin reduced sucrose transporter abundance at the plasma membrane and decreased uptake of the fluorescent sucrose analogue esculin. In Arabidopsis leaves, methyl-\u03b2-cyclodextrin treatment reduced endogenous AtSUC2 protein levels, confirming sterol-dependent regulation in planta. Single-molecule localization microscopy of AtSUC2\u2013reporter constructs revealed its enrichment in \u223c50\u00a0nm plasma membrane domains, with the localization becoming more homogeneous upon sterol depletion. Together, our results demonstrate that plasma membrane lipid composition modulates sucrose transporter localization and abundance, with likely relevance for the regulation of phloem loading and other membrane proteins.<\/jats:p>","DOI":"10.1093\/pcp\/pcag141","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T11:48:39Z","timestamp":1792583319000},"source":"Crossref","is-referenced-by-count":0,"title":["Membrane environment-dependent relocation of phloem-loading sucrose transporters"],"prefix":"10.1093","author":[{"given":"Shijiao","family":"Yin","sequence":"first","affiliation":[{"name":"College of Life Sciences, Northwest A&F University , 712100 Yangling ,","place":["China"]},{"name":"Institute of Biology, University of Graz , 8010 Graz ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biology, University of Graz , 8010 Graz ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Burt","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Humboldt University of Berlin , 10115 Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8370-9706","authenticated-orcid":false,"given":"Christina","family":"K\u00fchn","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Humboldt University of Berlin , 10115 Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8753-4037","authenticated-orcid":false,"given":"Johannes","family":"Liesche","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Northwest A&F University , 712100 Yangling ,","place":["China"]},{"name":"Institute of Biology, University of Graz , 8010 Graz ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,21]]},"container-title":["Plant and Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/pcp\/advance-article-pdf\/doi\/10.1093\/pcp\/pcag141\/72251228\/pcag141.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/pcp\/advance-article-pdf\/doi\/10.1093\/pcp\/pcag141\/72251228\/pcag141.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:56:12Z","timestamp":1792587372000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/pcp\/advance-article\/doi\/10.1093\/pcp\/pcag141\/8964914"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,21]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/pcp\/pcag141","relation":{},"ISSN":["0032-0781","1471-9053"],"issn-type":[{"value":"0032-0781","type":"print"},{"value":"1471-9053","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,21]]},"article-number":"pcag141"}}