{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:49:51Z","timestamp":1791319791321,"version":"4.3.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Arsenic is a toxic, carcinogenic metalloid that threatens human health through contaminated water and food. Arsenite [As(III)] efflux mediated by ACR3 transporters is an ancient detoxification mechanism conserved across prokaryotes, fungi, algae, and non-angiosperm land plants, yet plant ACR3 proteins remain poorly characterized. Here, we show that MpACR3, the ACR3 ortholog from the model liverwort Marchantia polymorpha, contains an arsenic-responsive N-terminal domain that regulates intracellular trafficking. Under non-stress conditions, MpACR3 is detected in Golgi bodies as well as at the plasma membrane in plant cells, whereas As(III) promotes its relocalization to the plasma membrane. As(III) interacts with a cysteine-rich N-terminal region and stabilizes this domain, as shown by arsenite-biotin competition and increased protease resistance. Together with molecular dynamics simulations, which predicts As(III)-dependent changes in secondary structure and surface properties, these findings support a model in which ligand-induced remodeling of the N terminus modulates MpACR3 retention and trafficking. We further identify a di-arginine-containing LRCRF motif in which leucine and arginine residues are required for intracellular retention, whereas cysteine and phenylalanine residues are required for As(III)-induced relocalization to the plasma membrane. The broad conservation of related N-terminal domains in plant ACR3 transporters suggests that arsenic-regulated trafficking is a conserved plant adaptation to arsenic toxicity.<\/jats:p>","DOI":"10.1093\/plcell\/koag306","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T11:36:40Z","timestamp":1791027400000},"source":"Crossref","is-referenced-by-count":0,"title":["Arsenic-sensing domain controls ACR3 transporter trafficking in\n                    <i>Marchantia polymorpha<\/i>"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9550-1689","authenticated-orcid":false,"given":"Ignacy","family":"Bonter","sequence":"first","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]},{"name":"Department of Plant Sciences, University of Cambridge , Downing Street, Cambridge, CB2 3EA ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5031-994X","authenticated-orcid":false,"given":"Katarzyna","family":"Mizio","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9540-4694","authenticated-orcid":false,"given":"Alicja","family":"Dolzblasz","sequence":"additional","affiliation":[{"name":"Department of Plant Developmental Biology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9596-7219","authenticated-orcid":false,"given":"Paulina","family":"Tomaszewska","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]},{"name":"Department of Genetics, Genomics and Cancer Sciences, University of Leicester , LE1 7RH, Leicester ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7451-4536","authenticated-orcid":false,"given":"Kacper","family":"Zbieralski","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6022-0350","authenticated-orcid":false,"given":"Jacek","family":"Staszewski","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-9907","authenticated-orcid":false,"given":"Donata","family":"Wawrzycka","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8458-7918","authenticated-orcid":false,"given":"Anna","family":"Reymer","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg , 405 30 G\u00f6teborg ,","place":["Sweden"]},{"name":"Institute for Advanced Biosciences (Inserm U1209) , Site Sant\u00e9 \u2013 Domaine de la Merci, 38700 La Tronche ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3390-5450","authenticated-orcid":false,"given":"Wojciech","family":"Bialek","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Biotechnology, University of Wroclaw , 50-383 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3782-5834","authenticated-orcid":false,"given":"Verena","family":"Kriechbaumer","sequence":"additional","affiliation":[{"name":"School of Biological and Medical Sciences and Centre for Bioimaging, Oxford Brookes University , Oxford OX3 0BP ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4793-8058","authenticated-orcid":false,"given":"Jim","family":"Haseloff","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of Cambridge , Downing Street, Cambridge, CB2 3EA ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3274-2629","authenticated-orcid":false,"given":"Robert","family":"Wysocki","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-7190","authenticated-orcid":false,"given":"Ewa","family":"Maciaszczyk-Dziubinska","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Physiology, Faculty of Biological Sciences, University of Wroclaw , 50-328 Wroclaw ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,6]]},"container-title":["The Plant Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/plcell\/advance-article-pdf\/doi\/10.1093\/plcell\/koag306\/71629178\/koag306.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plcell\/advance-article-pdf\/doi\/10.1093\/plcell\/koag306\/71629178\/koag306.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:50:37Z","timestamp":1791316237000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plcell\/advance-article\/doi\/10.1093\/plcell\/koag306\/8869226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/plcell\/koag306","relation":{},"ISSN":["1040-4651","1532-298X"],"issn-type":[{"value":"1040-4651","type":"print"},{"value":"1532-298X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"article-number":"koag306"}}