{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:40:35Z","timestamp":1781282435619,"version":"3.54.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272670"],"award-info":[{"award-number":["32272670"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972986"],"award-info":[{"award-number":["31972986"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS-1901566"],"award-info":[{"award-number":["IOS-1901566"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Grapevine (Vitis vinifera) is an economically important fruit crop worldwide. The widely cultivated grapevine is susceptible to powdery mildew caused by Erysiphe necator. In this study, we used CRISPR-Cas9 to simultaneously knock out VviWRKY10 and VviWRKY30 encoding two transcription factors reported to be implicated in defense regulation. We generated 53 wrky10 single mutant transgenic plants and 15 wrky10 wrky30 double mutant transgenic plants. In a 2-yr field evaluation of powdery mildew resistance, the wrky10 mutants showed strong resistance, while the wrky10 wrky30 double mutants showed moderate resistance. Further analyses revealed that salicylic acid (SA) and reactive oxygen species contents in the leaves of wrky10 and wrky10 wrky30 were substantially increased, as was the ethylene (ET) content in the leaves of wrky10. The results from dual luciferase reporter assays, electrophoretic mobility shift assays and chromatin immunoprecipitation (ChIP) assays demonstrated that VviWRKY10 could directly bind to the W-boxes in the promoter of SA-related defense genes and inhibit their transcription, supporting its role as a negative regulator of SA-dependent defense. By contrast, VviWRKY30 could directly bind to the W-boxes in the promoter of ET-related defense genes and promote their transcription, playing a positive role in ET production and ET-dependent defense. Moreover, VviWRKY10 and VviWRKY30 can bind to each other's promoters and mutually inhibit each other's transcription. Taken together, our results reveal a complex mechanism of regulation by VviWRKY10 and VviWRKY30 for activation of measured and balanced defense responses against powdery mildew in grapevine.<\/jats:p>","DOI":"10.1093\/plphys\/kiae080","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T06:15:40Z","timestamp":1708150540000},"page":"446-461","source":"Crossref","is-referenced-by-count":19,"title":["Transcription factors VviWRKY10 and VviWRKY30 co-regulate powdery mildew resistance in grapevine"],"prefix":"10.1093","volume":"195","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6847-3221","authenticated-orcid":false,"given":"Min","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0609-043X","authenticated-orcid":false,"given":"Hongyan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1418-5678","authenticated-orcid":false,"given":"Xuena","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1582-2734","authenticated-orcid":false,"given":"Kaicheng","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6383-1299","authenticated-orcid":false,"given":"Yang","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1348-4879","authenticated-orcid":false,"given":"Shunyuan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland , Rockville, MD 20850 , USA"},{"name":"Department of Plant Science and Landscape Architecture, University of Maryland, College Park , MD 20742 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-7260","authenticated-orcid":false,"given":"Ying-Qiang","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University , Yangling 712100, Shaanxi , China"},{"name":"Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs , Yangling 712100, Shaanxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"issue":"2","key":"2024043019242964500_kiae080-B1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/tpj.14124","article-title":"Differential regulation and interaction of homoeologous WRKY18 and WRKY40 in Arabidopsis allotetraploids and biotic stress responses","volume":"97","author":"Abeysinghe","year":"2019","journal-title":"Plant J"},{"issue":"1","key":"2024043019242964500_kiae080-B2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1105\/tpc.16.00681","article-title":"Induced genome-wide binding of three arabidopsis WRKY transcription factors during early MAMP-triggered 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