{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:39:09Z","timestamp":1792111149000,"version":"4.4.0"},"reference-count":88,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["290476080"],"award-info":[{"award-number":["290476080"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["469950637"],"award-info":[{"award-number":["469950637"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["496871662"],"award-info":[{"award-number":["496871662"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WI 3560\/7-1"],"award-info":[{"award-number":["WI 3560\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["He 1848\/20-1"],"award-info":[{"award-number":["He 1848\/20-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Leibniz Programme"},{"DOI":"10.13039\/100002014","name":"CRC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002014","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NSERC-CREATE"},{"name":"NSERC-Discovery funds"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In humans and plants, 40% of the proteome is cotranslationally acetylated at the N-terminus by a single N\u03b1-acetyltransferase (Nat) termed NatA. The core NatA complex is comprised of the catalytic subunit N\u03b1-acetyltransferase 10 (NAA10) and the ribosome-anchoring subunit NAA15. The regulatory subunit Huntingtin Yeast Partner K (HYPK) and the acetyltransferase NAA50 join this complex in humans. Even though both are conserved in Arabidopsis (Arabidopsis thaliana), only AtHYPK is known to interact with AtNatA. Here we uncover the AtNAA50 interactome and provide evidence for the association of AtNAA50 with NatA at ribosomes. In agreement with the latter, a split-luciferase approach demonstrated close proximity of AtNAA50 and AtNatA in planta. Despite their interaction, AtNatA\/HYPK and AtNAA50 exerted different functions in vivo. Unlike NatA\/HYPK, AtNAA50 did not modulate drought tolerance or promote protein stability. Instead, transcriptome and proteome analyses of a novel AtNAA50-depleted mutant (amiNAA50) implied that AtNAA50 negatively regulates plant immunity. Indeed, amiNAA50 plants exhibited enhanced resistance to oomycetes and bacterial pathogens. In contrast to what was observed in NatA-depleted mutants, this resistance was independent of an accumulation of salicylic acid prior to pathogen exposure. Our study dissects the in vivo function of the NatA interactors HYPK and NAA50 and uncovers NatA-independent roles for NAA50 in plants.<\/jats:p>","DOI":"10.1093\/plphys\/kiae200","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T17:59:20Z","timestamp":1712599160000},"page":"3097-3118","source":"Crossref","is-referenced-by-count":4,"title":["N\u03b1-acetyltransferase NAA50 mediates plant immunity independent of the N\u03b1-acetyltransferase A complex"],"prefix":"10.1093","volume":"195","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4559-2404","authenticated-orcid":false,"given":"Laura","family":"Armbruster","sequence":"first","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5997-4879","authenticated-orcid":false,"given":"Marlena","family":"Po\u017coga","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6687-1675","authenticated-orcid":false,"given":"Zhongshou","family":"Wu","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia , V6T1Z4 Vancouver, BC , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0963-1872","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Eirich","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology and Biotechnology, University of M\u00fcnster , 48149 M\u00fcnster , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6652-5032","authenticated-orcid":false,"given":"Karen","family":"Thulasi Devendrakumar","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia , V6T1Z4 Vancouver, BC , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8918-2266","authenticated-orcid":false,"given":"Carolina","family":"De La Torre","sequence":"additional","affiliation":[{"name":"NGS Core Facility, Medical Faculty Mannheim of Heidelberg University , 68167 Mannheim , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1170-5490","authenticated-orcid":false,"given":"Pavlina","family":"Mikl\u00e1nkov\u00e1","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3127-2100","authenticated-orcid":false,"given":"Monika","family":"Huber","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9811-9453","authenticated-orcid":false,"given":"Fabian","family":"Bradic","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-0865","authenticated-orcid":false,"given":"Gernot","family":"Poschet","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8376-2083","authenticated-orcid":false,"given":"Jonas","family":"Weidenhausen","sequence":"additional","affiliation":[{"name":"Structural Biology, Heidelberg University Biochemistry Center , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4052-9927","authenticated-orcid":false,"given":"Sabine","family":"Merker","sequence":"additional","affiliation":[{"name":"Core Facility for Mass Spectrometry and Proteomics , Center for Molecular Biology of Heidelberg University, 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9152-2266","authenticated-orcid":false,"given":"Thomas","family":"Ruppert","sequence":"additional","affiliation":[{"name":"Core Facility for Mass Spectrometry and Proteomics , Center for Molecular Biology of Heidelberg University, 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3596-6094","authenticated-orcid":false,"given":"Carsten","family":"Sticht","sequence":"additional","affiliation":[{"name":"NGS Core Facility, Medical Faculty Mannheim of Heidelberg University , 68167 Mannheim , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9127-4477","authenticated-orcid":false,"given":"Irmgard","family":"Sinning","sequence":"additional","affiliation":[{"name":"Structural Biology, Heidelberg University Biochemistry Center , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8972-4026","authenticated-orcid":false,"given":"Iris","family":"Finkemeier","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology and Biotechnology, University of M\u00fcnster , 48149 M\u00fcnster , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6354-2021","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia , V6T1Z4 Vancouver, BC , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6238-4818","authenticated-orcid":false,"given":"R\u00fcdiger","family":"Hell","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7790-4022","authenticated-orcid":false,"given":"Markus","family":"Wirtz","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University , 69120 Heidelberg , 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