{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T08:45:13Z","timestamp":1791794713835,"version":"4.3.4"},"reference-count":102,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Engineering pyrenoid-based CO2-concentrating mechanisms (pCCMs) into crop plants is a promising strategy to boost photosynthetic performance, enhance yield potential, and improve resilience to future climates. Achieving this goal requires a deeper understanding of the molecular principles that enable pyrenoids to elevate CO2 around Rubisco. Although pyrenoids span a remarkable diversity of forms across algae and hornworts, they consistently exhibit 3 core features: a condensed Rubisco matrix, specialized membranes that deliver inorganic carbon, and a diffusion barrier that restricts CO2 leakage. Here, we review recent mechanistic advances from Chlamydomonas reinhardtii alongside emerging insights from other algae and hornworts that highlight both conserved strategies and lineage-specific innovations in Rubisco condensation, membrane-associated inorganic carbon channelling, and matrix encapsulation. Together, these findings refine our understanding of pCCM diversity and provide an increasingly robust blueprint for reconstructing pCCMs in vascular plant chloroplasts.<\/jats:p>","DOI":"10.1093\/plphys\/kiag250","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T11:24:53Z","timestamp":1777289093000},"source":"Crossref","is-referenced-by-count":3,"title":["Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering"],"prefix":"10.1093","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9770-8444","authenticated-orcid":false,"given":"Jessica H","family":"Pritchard","sequence":"first","affiliation":[{"name":"Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]},{"name":"Centre for Engineering Biology, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5080-2904","authenticated-orcid":false,"given":"Sophie N R","family":"Young","sequence":"additional","affiliation":[{"name":"Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]},{"name":"Centre for Engineering Biology, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7650-0189","authenticated-orcid":false,"given":"Herbie","family":"Garland","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products (CNAP), Department of Biology, University of York , Heslington, York, YO10 5DD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1440-3233","authenticated-orcid":false,"given":"Luke C M","family":"Mackinder","sequence":"additional","affiliation":[{"name":"Centre for Novel Agriculture Products (CNAP), Department of Biology, University of York , Heslington, York, YO10 5DD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7255-872X","authenticated-orcid":false,"given":"Alistair J","family":"McCormick","sequence":"additional","affiliation":[{"name":"Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]},{"name":"Centre for Engineering Biology, University of Edinburgh , Edinburgh, EH9 3BF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"2026101203565186100_kiag250-B1","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1093\/plphys\/kiac373","article-title":"New horizons for building 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