{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:18:23Z","timestamp":1790252303155,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan","doi-asserted-by":"crossref","award":["232300421112"],"award-info":[{"award-number":["232300421112"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 32472662"],"award-info":[{"award-number":["NSFC: 32472662"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Previous studies have shown that hydrogen peroxide (H2O2) treatment promotes early ripening in \u201cKyoho\u201d grapes (Vitis vinifera \u00d7 Vitis labrusca); however, the mechanism by which H2O2 oxidation modification regulates grape ripening remains unclear. Here, RNA-Seq data revealed substantial downregulation of small Heat Shock Protein 20-43 (VvHSP20-43) expression levels in the treated group. Co-expression network analysis identified MYB Altered Phloem Development (VvMYB APL) as a key regulator of VvHSP20-43 expression. Dual luciferase, yeast one-hybrid, and electrophoretic mobility shift assays confirmed that VvMYB APL binds to the VvHSP20-43 promoter, thereby activating its transcriptional expression. Stable transformation in Arabidopsis and transient transformation in grape berries demonstrated that VvMYB APL and VvHSP20-43 negatively regulate fruit ripening. Further analysis revealed that exogenous H2O2 treatment rapidly oxidizes the VvMYB APL protein, inducing methionine (Met) site mutations that inhibit its transcriptional activation of VvHSP20-43. In addition, reduced expression of VvMYB APL and VvHSP20-43 promoted the expression of ripening-related genes, thereby accelerating grape berry ripening. This study elucidates the molecular mechanism by which H2O2 oxidizes the VvMYB APL transcription factor, thereby downregulating VvHSP20-43 expression and influencing fruit ripening.<\/jats:p>","DOI":"10.1093\/plphys\/kiag454","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T18:44:41Z","timestamp":1782758681000},"source":"Crossref","is-referenced-by-count":1,"title":["H2O2 oxidation of VvMYB APL reduces\n                    <i>VvHSP20-43<\/i>\n                    expression and promotes grape ripening"],"prefix":"10.1093","volume":"201","author":[{"given":"Jia-Lin","family":"Xing","sequence":"first","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding-Ding","family":"Zuo","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"College of Agronomy\/Tree Peony, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Ya","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy\/Tree Peony, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Ling","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-6537","authenticated-orcid":false,"given":"Hai-Nan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Henan University of Science and Technology , Luoyang, Henan 471023 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3650-3115","authenticated-orcid":false,"given":"Mao-Song","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant 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