{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:35:14Z","timestamp":1789594514624,"version":"build-2803163510"},"reference-count":94,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"DFG","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["SFB1535"],"award-info":[{"award-number":["SFB1535"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["458090666"],"award-info":[{"award-number":["458090666"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Diurnal changes in light availability are a defining feature of life on Earth. Photoautotrophic organisms therefore store reduced carbon during the day to sustain energy metabolism at night. In cyanobacteria, glycogen is the primary carbon storage compound and supports both energy homeostasis and stress responses. Although glycogen-deficient Synechocystis strains have been studied previously, how these mutants cope with the loss of the major daytime carbon sink and can sustain themselves during the night remains unclear. Using single-cell microfluidics, transcriptomics, and metabolomics, we show that \u0394glgC mutants exhibit pronounced light sensitivity. At sublethal light intensities, daytime transcriptional responses are dominated by downregulation of photosynthesis-related genes, likely preventing NADPH overaccumulation in the absence of a carbon sink. During the night, mutants display severe energy limitation, characterized by reduced ATP levels, altered redox balance, and depletion of central carbon intermediates. In contrast, fumarate and malate accumulate, indicating enhanced respiratory flux through succinate dehydrogenase. These metabolic constraints lead to extended lag phases and delayed cell divisions after the onset of light, demonstrating that glycogen-deficient cells fail to efficiently reinitiate growth after dawn. Overall, our results as a snapshot of the initial response to diurnal regimes highlight glycogen as a central integrator of diurnal physiology in Synechocystis, coordinating energy metabolism, redox balance, and cell division, with implications for metabolic robustness and the evolutionary constraints shaping (endo)symbiosis.<\/jats:p>","DOI":"10.1093\/plphys\/kiag560","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T11:20:28Z","timestamp":1785324028000},"source":"Crossref","is-referenced-by-count":1,"title":["Glycogen deficiency impairs diurnal energy metabolism and cell division in\n                    <i>Synechocystis<\/i>"],"prefix":"10.1093","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2044-7462","authenticated-orcid":false,"given":"Jan M","family":"Hofer","sequence":"first","affiliation":[{"name":"Institute for Plant Biochemistry, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7570-0913","authenticated-orcid":false,"given":"Tim","family":"Schulze","sequence":"additional","affiliation":[{"name":"Faculty of Biology\/Computational Biology, Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]},{"name":"Center for Biotechnology (CeBiTec), Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3980-7577","authenticated-orcid":false,"given":"Lennart","family":"Witting","sequence":"additional","affiliation":[{"name":"IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH , Wilhelm-Johnen-Str., 52428 J\u00fclich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5792-0102","authenticated-orcid":false,"given":"Bianca","family":"Laker","sequence":"additional","affiliation":[{"name":"Faculty of Biology\/Computational Biology, Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]},{"name":"Center for Biotechnology (CeBiTec), Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Krueger","sequence":"additional","affiliation":[{"name":"Institute for Plant Biochemistry, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]},{"name":"CEPLAS Metabolism and Metabolomics Laboratory, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-9420","authenticated-orcid":false,"given":"Philipp","family":"Westhoff","sequence":"additional","affiliation":[{"name":"Institute for Plant Biochemistry, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]},{"name":"CEPLAS Metabolism and Metabolomics Laboratory, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6215-1857","authenticated-orcid":false,"given":"Dietrich","family":"Kohlheyer","sequence":"additional","affiliation":[{"name":"IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH , Wilhelm-Johnen-Str., 52428 J\u00fclich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-4672","authenticated-orcid":false,"given":"Andreas P M","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Plant Biochemistry, Heinrich-Heine University D\u00fcsseldorf , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf ,","place":["Germany"]},{"name":"Heinrich-Heine University D\u00fcsseldorf Cluster of Excellence on Plant Science (CEPLAS), , Universit\u00e4tsstr. 1, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2743-8630","authenticated-orcid":false,"given":"Marion","family":"Eisenhut","sequence":"additional","affiliation":[{"name":"Faculty of Biology\/Computational Biology, Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]},{"name":"Center for Biotechnology (CeBiTec), Bielefeld University , Universit\u00e4tsstr. 27, 33615 Bielefeld ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"2026090414264831700_kiag560-B1","unstructured":"Alexa A, Rahnenfuhrer J. 2026. topGO: enrichment analysis for gene ontology. Version 2.62.0. Bioconductor. https:\/\/bioconductor.org\/packages\/topGO."},{"key":"2026090414264831700_kiag560-B2","doi-asserted-by":"publisher","first-page":"2761","DOI":"10.1128\/jb.173.9.2761-2767.1991","article-title":"Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process","volume":"173","author":"Anderson","year":"1991","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B3","unstructured":"Andrews \u00a0S\n          . 2010. FastQC: a quality control tool for high throughput sequence data. Version 0.12.1. Babraham Bioinformatics. https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"2026090414264831700_kiag560-B4","doi-asserted-by":"publisher","first-page":"4180","DOI":"10.1128\/AEM.00256-16","article-title":"Culturing Synechocystis sp. strain PCC 6803 with N2 and CO2 in a diel regime reveals multiphase glycogen dynamics with low maintenance costs","volume":"82","author":"Angermayr","year":"2016","journal-title":"Appl Environ Microbiol"},{"key":"2026090414264831700_kiag560-B5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/978-4-431-55495-0_4","volume-title":"Starch","author":"Ball","year":"2015"},{"key":"2026090414264831700_kiag560-B6","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1093\/jxb\/erq411","article-title":"The evolution of glycogen and starch metabolism in eukaryotes gives molecular clues to understand the establishment of plastid endosymbiosis","volume":"62","author":"Ball","year":"2011","journal-title":"J Exp Bot"},{"key":"2026090414264831700_kiag560-B7","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1146\/annurev.arplant.54.031902.134927","article-title":"From bacterial glycogen to starch: understanding the biogenesis of the plant starch granule","volume":"54","author":"Ball","year":"2003","journal-title":"Annu Rev Plant Biol"},{"key":"2026090414264831700_kiag560-B8","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.ceb.2020.10.013","article-title":"FtsZ dynamics in bacterial division: what, how, and why?","volume":"68","author":"Barrows","year":"2021","journal-title":"Curr Opin Cell Biol"},{"key":"2026090414264831700_kiag560-B9","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1016\/j.bbabio.2010.10.017","article-title":"Cyanobacterial NDH-1 complexes: novel insights and remaining puzzles","volume":"1807","author":"Battchikova","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"2026090414264831700_kiag560-B10","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1186\/1471-2164-13-56","article-title":"The diversity of cyanobacterial metabolism: genome analysis of multiple phototrophic microorganisms","volume":"13","author":"Beck","year":"2012","journal-title":"BMC Genomics"},{"key":"2026090414264831700_kiag560-B11","doi-asserted-by":"publisher","first-page":"475","DOI":"10.3389\/fmicb.2016.00475","article-title":"Factors altering pyruvate excretion in a glycogen storage mutant of the cyanobacterium, synechococcus PCC7942","volume":"7","author":"Benson","year":"2016","journal-title":"Front Microbiol"},{"key":"2026090414264831700_kiag560-B12","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgaf120","article-title":"Self-sustained rhythmic behavior of Synechocystis sp. PCC 6803 under continuous light conditions in the absence of light-dark entrainment","volume":"4","author":"Berwanger","year":"2025","journal-title":"PNAS Nexus"},{"key":"2026090414264831700_kiag560-B13","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/B978-0-12-416687-5.00006-3","volume-title":"Essentials of medical biochemistry","author":"Bhagavan","year":"2015"},{"key":"2026090414264831700_kiag560-B14","doi-asserted-by":"publisher","first-page":"14746","DOI":"10.1073\/pnas.1207726109","article-title":"Gene balance hypothesis: connecting issues of dosage sensitivity across biological disciplines","volume":"109","author":"Birchler","year":"2012","journal-title":"Proc Natl Acad Sci USA."},{"key":"2026090414264831700_kiag560-B15","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2026090414264831700_kiag560-B16","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1038\/nbt.3519","article-title":"Near-optimal probabilistic RNA-Seq quantification","volume":"34","author":"Bray","year":"2016","journal-title":"Nat Biotechnol"},{"key":"2026090414264831700_kiag560-B17","doi-asserted-by":"publisher","first-page":"8144","DOI":"10.1128\/JB.186.23.8144-8148.2004","article-title":"The malic enzyme is required for optimal photoautotrophic growth of Synechocystis sp. strain PCC 6803 under continuous light but not under a diurnal light regimen","volume":"186","author":"Bricker","year":"2004","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B18","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2026090414264831700_kiag560-B19","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1016\/j.celrep.2018.03.083","article-title":"Glycogen synthesis and metabolite overflow contribute to energy balancing in Cyanobacteria","volume":"23","author":"Cano","year":"2018","journal-title":"Cell Rep"},{"key":"2026090414264831700_kiag560-B20","doi-asserted-by":"publisher","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-mapper v2: functional annotation, orthology assignments, and domain prediction at the metagenomic scale","volume":"38","author":"Cantalapiedra","year":"2021","journal-title":"Mol Biol Evol"},{"key":"2026090414264831700_kiag560-B21","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.algal.2016.11.003","article-title":"Transcriptome and proteome analysis of nitrogen starvation responses in Synechocystis 6803 \u0394glgC, a mutant incapable of glycogen storage","volume":"21","author":"Carrieri","year":"2017","journal-title":"Algal Res"},{"key":"2026090414264831700_kiag560-B22","doi-asserted-by":"publisher","first-page":"9457","DOI":"10.1039\/c2ee23181f","article-title":"Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage","volume":"5","author":"Carrieri","year":"2012","journal-title":"Energy Environ Sci"},{"key":"2026090414264831700_kiag560-B23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf018","article-title":"edgeR v4: powerful differential analysis of sequencing data with expanded functionality and improved support for small counts and larger datasets","volume":"53","author":"Chen","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026090414264831700_kiag560-B24","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1093\/molbev\/msq158","article-title":"Phylogenetic and biochemical evidence supports the recruitment of an ADP-glucose translocator for the export of photosynthate during plastid endosymbiosis","volume":"27","author":"Colleoni","year":"2010","journal-title":"Mol Biol Evol"},{"key":"2026090414264831700_kiag560-B25","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/978-3-030-60173-7_8","volume-title":"Bacterial organelles and organelle-like inclusions. Microbiology monographs","author":"Colpaert","year":"2020"},{"key":"2026090414264831700_kiag560-B26","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1128\/JB.182.3.714-722.2000","article-title":"Succinate:quinol oxidoreductases in the cyanobacterium Synechocystis sp. strain PCC 6803: presence and function in metabolism and electron transport","volume":"182","author":"Cooley","year":"2000","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B27","doi-asserted-by":"publisher","first-page":"4251","DOI":"10.1128\/JB.183.14.4251-4258.2001","article-title":"Succinate dehydrogenase and other respiratory pathways in thylakoid membranes of Synechocystis sp. strain PCC 6803: capacity comparisons and physiological function","volume":"183","author":"Cooley","year":"2001","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B28","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1073\/pnas.87.3.1129","article-title":"Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems","volume":"87","author":"de Boer","year":"1990","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026090414264831700_kiag560-B29","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1093\/molbev\/msm280","article-title":"Metabolic symbiosis and the birth of the plant kingdom","volume":"25","author":"Deschamps","year":"2008","journal-title":"Mol Biol Evol"},{"key":"2026090414264831700_kiag560-B30","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1093\/jxb\/erz559","article-title":"Lethality caused by ADP-glucose accumulation is suppressed by salt-induced carbon flux redirection in cyanobacteria","volume":"71","author":"D\u00edaz-Troya","year":"2020","journal-title":"J Exp Bot"},{"key":"2026090414264831700_kiag560-B31","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1093\/plphys\/kiae083","article-title":"Double blocking of carbon metabolism causes a large increase of Calvin\u2013Benson cycle compounds in cyanobacteria","volume":"195","author":"Dom\u00ednguez-Lobo","year":"2024","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B32","doi-asserted-by":"publisher","first-page":"2660","DOI":"10.1128\/AEM.07901-11","article-title":"Rerouting carbon flux to enhance photosynthetic productivity","volume":"78","author":"Ducat","year":"2012","journal-title":"Appl Environ Microbiol"},{"key":"2026090414264831700_kiag560-B33","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/BF02187472","article-title":"Cloning and sequencing of mutant psbB genes of the cyanobacterium Synechocystis PCC 6803","volume":"37","author":"Ermakova","year":"1993","journal-title":"Photosynth Res"},{"key":"2026090414264831700_kiag560-B34","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/j.tplants.2013.09.006","article-title":"Chlamydia, cyanobiont, or host: who was on top in the m\u00e9nage \u00e0 trois?","volume":"18","author":"Facchinelli","year":"2013","journal-title":"Trends Plant Sci"},{"key":"2026090414264831700_kiag560-B35","doi-asserted-by":"publisher","first-page":"D279","DOI":"10.1093\/nar\/gkv1344","article-title":"The Pfam protein families database: towards a more sustainable future","volume":"44","author":"Finn","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"2026090414264831700_kiag560-B36","doi-asserted-by":"publisher","first-page":"2767","DOI":"10.3390\/microorganisms13122767","article-title":"Controlled carbon loss: threshold-dependent overflow metabolism in Synechocystis sp. PCC 6803","volume":"13","author":"Forchhammer","year":"2025","journal-title":"Microorganisms"},{"key":"2026090414264831700_kiag560-B37","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00063-22","article-title":"Heterologous lactate synthesis in Synechocystis sp. strain PCC 6803 causes a growth condition-dependent carbon sink effect","volume":"88","author":"Grund","year":"2022","journal-title":"Appl Environ Microbiol"},{"key":"2026090414264831700_kiag560-B38","doi-asserted-by":"publisher","first-page":"3032","DOI":"10.1099\/mic.0.062950-0","article-title":"Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803","volume":"158","author":"Gr\u00fcndel","year":"2012","journal-title":"Microbiology (Reading)"},{"key":"2026090414264831700_kiag560-B39","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/S0168-9525(02)02734-8","article-title":"Glycogen metabolism loss: a common marker of parasitic behaviour in bacteria?","volume":"18","author":"Henrissat","year":"2002","journal-title":"Trends Genet"},{"key":"2026090414264831700_kiag560-B40","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.algal.2013.01.008","article-title":"Glycogen synthesis is a required component of the nitrogen stress response in Synechococcus elongatus PCC 7942","volume":"2","author":"Hickman","year":"2013","journal-title":"Algal Res"},{"key":"2026090414264831700_kiag560-B41","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.algal.2016.09.021","article-title":"Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance","volume":"20","author":"Holland","year":"2016","journal-title":"Algal Res"},{"key":"2026090414264831700_kiag560-B42","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1093\/pcp\/pcz044","article-title":"Thylakoid localized type 2 NAD(P)H dehydrogenase NdbA optimizes light-activated heterotrophic growth of Synechocystis sp. PCC 6803","volume":"60","author":"Huokko","year":"2019","journal-title":"Plant Cell Physiol"},{"key":"2026090414264831700_kiag560-B43","doi-asserted-by":"publisher","first-page":"1863","DOI":"10.1104\/pp.17.00398","article-title":"Role of type 2 NAD(P)H dehydrogenase NdbC in redox regulation of carbon allocation in Synechocystis","volume":"174","author":"Huokko","year":"2017","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B44","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1093\/pcp\/pcab023","article-title":"The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803","volume":"62","author":"Ishikawa","year":"2021","journal-title":"Plant Cell Physiol"},{"key":"2026090414264831700_kiag560-B45","doi-asserted-by":"publisher","first-page":"6210","DOI":"10.1128\/AEM.01751-15","article-title":"Dynamics of photosynthesis in a glycogen-deficient glgC mutant of Synechococcus sp. strain PCC 7002","volume":"81","author":"Jackson","year":"2015","journal-title":"Appl Environ Microbiol"},{"key":"2026090414264831700_kiag560-B46","doi-asserted-by":"publisher","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"2026090414264831700_kiag560-B47","doi-asserted-by":"publisher","first-page":"10208","DOI":"10.1073\/pnas.1421375112","article-title":"Metabolic connectivity as a driver of host and endosymbiont integration","volume":"112","author":"Karkar","year":"2015","journal-title":"Proc Natl Acad Sci USA."},{"key":"2026090414264831700_kiag560-B48","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02187-22","article-title":"Malic enzyme, not malate dehydrogenase, mainly oxidizes malate that originates from the tricarboxylic acid cycle in Cyanobacteria","volume":"13","author":"Katayama","year":"2022","journal-title":"mBio"},{"key":"2026090414264831700_kiag560-B49","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1038\/s42003-024-05929-9","article-title":"Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria","volume":"7","author":"Kato","year":"2024","journal-title":"Commun Biol"},{"key":"2026090414264831700_kiag560-B50","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1104\/pp.110.166652","article-title":"The importance of energy balance in improving photosynthetic productivity","volume":"155","author":"Kramer","year":"2011","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B51","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.bbabio.2015.10.007","article-title":"Photosynthetic, respiratory and extracellular electron transport pathways in cyanobacteria","volume":"1857","author":"Lea-Smith","year":"2016","journal-title":"Biochim Biophys Acta."},{"key":"2026090414264831700_kiag560-B52","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1111\/febs.17348","article-title":"Deciphering the tight metabolite-level regulation of glucose-1-phosphate adenylyltransferase (GlgC) for glycogen synthesis in cyanobacteria","volume":"292","author":"Lee","year":"2025","journal-title":"FEBS J"},{"key":"2026090414264831700_kiag560-B53","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1007\/s11120-014-9987-6","article-title":"Isobutanol production as an alternative metabolic sink to rescue the growth deficiency of the glycogen mutant of Synechococcus elongatus PCC 7942","volume":"120","author":"Li","year":"2014","journal-title":"Photosynth Res"},{"key":"2026090414264831700_kiag560-B54","doi-asserted-by":"publisher","first-page":"3911","DOI":"10.1016\/j.cub.2022.07.035","article-title":"Connecting single-cell ATP dynamics to overflow metabolism, cell growth, and the cell cycle in Escherichia coli","volume":"32","author":"Lin","year":"2022","journal-title":"Curr Biol"},{"key":"2026090414264831700_kiag560-B55","doi-asserted-by":"publisher","first-page":"1417680","DOI":"10.3389\/fpls.2024.1417680","article-title":"The primary carbon metabolism in cyanobacteria and its regulation","volume":"15","author":"Lucius","year":"2024","journal-title":"Front Plant Sci"},{"key":"2026090414264831700_kiag560-B56","doi-asserted-by":"publisher","first-page":"787943","DOI":"10.3389\/fpls.2021.787943","article-title":"The Entner-Doudoroff pathway contributes to glycogen breakdown during high to low CO2 shifts in the Cyanobacterium Synechocystis sp. PCC 6803","volume":"12","author":"Lucius","year":"2021","journal-title":"Front Plant Sci"},{"key":"2026090414264831700_kiag560-B57","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.molp.2020.02.002","article-title":"Glycolytic shunts replenish the Calvin-Benson-Bassham cycle as anaplerotic reactions in Cyanobacteria","volume":"13","author":"Makowka","year":"2020","journal-title":"Mol Plant"},{"key":"2026090414264831700_kiag560-B58","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1111\/j.1365-2958.2004.04042.x","article-title":"Molecular analysis of the key cytokinetic components of cyanobacteria: FtsZ, ZipN and MinCDE","volume":"52","author":"Mazouni","year":"2004","journal-title":"Mol Microbiol"},{"key":"2026090414264831700_kiag560-B59","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1023\/A:1022446330284","article-title":"Changes in photosynthesis and pigmentation in an agp deletion mutant of the Cyanobacterium Synechocystis sp","volume":"25","author":"Miao","year":"2003","journal-title":"Biotechnol Lett."},{"key":"2026090414264831700_kiag560-B60","doi-asserted-by":"publisher","first-page":"1340413","DOI":"10.3389\/fmicb.2024.1340413","article-title":"CyanoCyc cyanobacterial web portal","volume":"15","author":"Moore","year":"2024","journal-title":"Front Microbiol"},{"key":"2026090414264831700_kiag560-B61","doi-asserted-by":"publisher","first-page":"10183","DOI":"10.1073\/pnas.93.19.10183","article-title":"Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours","volume":"93","author":"Mori","year":"1996","journal-title":"Proc Natl Acad Sci USA."},{"key":"2026090414264831700_kiag560-B62","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3389\/fpls.2014.00007","article-title":"Electron transport and light-harvesting switches in cyanobacteria","volume":"5","author":"Mullineaux","year":"2014","journal-title":"Front Plant Sci"},{"key":"2026090414264831700_kiag560-B63","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1186\/s12934-024-02462-6","article-title":"Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches","volume":"23","author":"Muth-Pawlak","year":"2024","journal-title":"Microb Cell Factories"},{"key":"2026090414264831700_kiag560-B64","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1186\/s12934-021-01622-2","article-title":"Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number","volume":"20","author":"Nagy","year":"2021","journal-title":"Microb Cell Fact"},{"key":"2026090414264831700_kiag560-B65","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1093\/pcp\/pci045","article-title":"Some Cyanobacteria synthesize semi-amylopectin type \u03b1-polyglucans instead of glycogen","volume":"46","author":"Nakamura","year":"2005","journal-title":"Plant Cell Physiol"},{"key":"2026090414264831700_kiag560-B66","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.cub.2008.02.051","article-title":"Chromatophore genome sequence of Paulinella sheds light on acquisition of photosynthesis by eukaryotes","volume":"18","author":"Nowack","year":"2008","journal-title":"Curr Biol"},{"key":"2026090414264831700_kiag560-B67","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1111\/nph.19793","article-title":"Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803","volume":"243","author":"Ortega-Mart\u00ednez","year":"2024","journal-title":"New Phytol"},{"key":"2026090414264831700_kiag560-B68","doi-asserted-by":"publisher","first-page":"1532","DOI":"10.1093\/jxb\/erac474","article-title":"Stress response requires an efficient connection between glycogen and central carbon metabolism by phosphoglucomutases in cyanobacteria","volume":"74","author":"Ortega-Mart\u00ednez","year":"2023","journal-title":"J Exp Bot"},{"key":"2026090414264831700_kiag560-B69","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.1016\/j.cub.2014.07.022","article-title":"Rhythms in energy storage control the ability of the cyanobacterial circadian clock to reset","volume":"24","author":"Pattanayak","year":"2014","journal-title":"Curr Biol"},{"key":"2026090414264831700_kiag560-B70","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1073\/pnas.1212113110","article-title":"Robust and tunable circadian rhythms from differentially sensitive catalytic domains","volume":"110","author":"Phong","year":"2013","journal-title":"Proc Natl Acad Sci USA."},{"key":"2026090414264831700_kiag560-B71","doi-asserted-by":"publisher","first-page":"6630","DOI":"10.1128\/JB.183.22.6630-6635.2001","article-title":"Escherichia coli division inhibitor MinCD blocks septation by preventing Z-ring formation","volume":"183","author":"Pichoff","year":"2001","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B72","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1146\/annurev.mi.38.100184.002223","article-title":"Bacterial glycogen synthesis and its regulation","volume":"38","author":"Preiss","year":"1984","journal-title":"Annu Rev Microbiol"},{"key":"2026090414264831700_kiag560-B73","doi-asserted-by":"publisher","first-page":"6167","DOI":"10.1021\/ac070470c","article-title":"Acidic acetonitrile for cellular metabolome extraction from Escherichia coli","volume":"79","author":"Rabinowitz","year":"2007","journal-title":"Anal Chem"},{"key":"2026090414264831700_kiag560-B74","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2025"},{"key":"2026090414264831700_kiag560-B75","doi-asserted-by":"publisher","first-page":"638255","DOI":"10.3389\/fbinf.2021.638255","article-title":"Summary visualizations of gene ontology terms with GO-figure!","volume":"1","author":"Reijnders","year":"2021","journal-title":"Front Bioinforma"},{"key":"2026090414264831700_kiag560-B76","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1617508114","article-title":"Cellular trade-offs and optimal resource allocation during cyanobacterial diurnal growth","volume":"114","author":"Reimers","year":"2017","journal-title":"Proc Natl Acad Sci USA."},{"key":"2026090414264831700_kiag560-B77","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1099\/00221287-111-1-1","article-title":"Generic assignments, strain histories and properties of pure cultures of Cyanobacteria","volume":"111","author":"Rippka","year":"1979","journal-title":"Microbiology"},{"key":"2026090414264831700_kiag560-B78","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1016\/j.cub.2005.06.040","article-title":"Monophyly of primary photosynthetic eukaryotes: green plants, red Algae, and glaucophytes","volume":"15","author":"Rodr\u00edguez-Ezpeleta","year":"2005","journal-title":"Curr Biol"},{"key":"2026090414264831700_kiag560-B79","doi-asserted-by":"publisher","first-page":"3344","DOI":"10.1128\/JB.185.11.3344-3351.2003","article-title":"Concentration and assembly of the division ring proteins FtsZ, FtsA, and ZipA during the Escherichia coli cell cycle","volume":"185","author":"Rueda","year":"2003","journal-title":"J Bacteriol"},{"key":"2026090414264831700_kiag560-B80","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1186\/s12859-019-2988-x","article-title":"FunFam protein families improve residue level molecular function prediction","volume":"20","author":"Scheibenreif","year":"2019","journal-title":"BMC Bioinformatics"},{"key":"2026090414264831700_kiag560-B81","doi-asserted-by":"publisher","first-page":"7667","DOI":"10.1021\/acs.analchem.7b01624","article-title":"Anion-exchange chromatography coupled to high-resolution mass spectrometry: a powerful tool for merging targeted and non-targeted metabolomics","volume":"89","author":"Schwaiger","year":"2017","journal-title":"Anal Chem"},{"key":"2026090414264831700_kiag560-B82","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/0092-8674(87)90221-2","article-title":"ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome","volume":"50","author":"Sekimizu","year":"1987","journal-title":"Cell"},{"key":"2026090414264831700_kiag560-B83","doi-asserted-by":"publisher","first-page":"1997","DOI":"10.1080\/09168451.2014.943648","article-title":"Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803","volume":"78","author":"Shimakawa","year":"2014","journal-title":"Biosci Biotechnol Biochem"},{"key":"2026090414264831700_kiag560-B84","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1104\/pp.19.01184","article-title":"Glycogen metabolism supports photosynthesis start through the oxidative pentose phosphate pathway in Cyanobacteria","volume":"182","author":"Shinde","year":"2020","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B85","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1104\/pp.119.1.267","article-title":"Modification of carbon partitioning, photosynthetic capacity, and O2 sensitivity in Arabidopsis plants with low ADP-glucose pyrophosphorylase activity","volume":"119","author":"Sun","year":"1999","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B86","doi-asserted-by":"publisher","first-page":"3153","DOI":"10.1128\/AEM.00397-08","article-title":"Carbohydrate metabolism in mutants of the Cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis","volume":"76","author":"Suzuki","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"2026090414264831700_kiag560-B87","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1093\/pcp\/pcs190","article-title":"Physicochemical variation of cyanobacterial starch, the insoluble \u03b1-glucans in Cyanobacteria","volume":"54","author":"Suzuki","year":"2013","journal-title":"Plant Cell Physiol"},{"key":"2026090414264831700_kiag560-B88","doi-asserted-by":"publisher","first-page":"41080","DOI":"10.1038\/srep41080","article-title":"Allosteric inhibition of phosphoenolpyruvate carboxylases is determined by a single amino acid residue in Cyanobacteria","volume":"7","author":"Takeya","year":"2017","journal-title":"Sci Rep"},{"key":"2026090414264831700_kiag560-B89","doi-asserted-by":"publisher","first-page":"2400","DOI":"10.1093\/plphys\/kiac602","article-title":"Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria","volume":"191","author":"Tanaka","year":"2023","journal-title":"Plant Physiol"},{"key":"2026090414264831700_kiag560-B90","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.tim.2018.11.002","article-title":"A hard Day's night: cyanobacteria in diel cycles","volume":"27","author":"Welkie","year":"2019","journal-title":"Trends Microbiol."},{"key":"2026090414264831700_kiag560-B91","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: elegant graphics for data analysis","author":"Wickham","year":"2016"},{"key":"2026090414264831700_kiag560-B92","doi-asserted-by":"publisher","first-page":"D380","DOI":"10.1093\/nar\/gkn762","article-title":"SUPERFAMILY\u2014sophisticated comparative genomics, data mining, visualization and phylogeny","volume":"37","author":"Wilson","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"2026090414264831700_kiag560-B93","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1039\/d4lc00567h","article-title":"A microfluidic system for the cultivation of cyanobacteria with precise light intensity and CO2 control: enabling growth data acquisition at single-cell resolution","volume":"25","author":"Witting","year":"2025","journal-title":"Lab Chip"},{"key":"2026090414264831700_kiag560-B94","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.ymben.2012.12.002","article-title":"Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: cell factories for soluble sugars","volume":"16","author":"Xu","year":"2013","journal-title":"Metab Eng"}],"container-title":["Plant Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/plphys\/advance-article-pdf\/doi\/10.1093\/plphys\/kiag560\/70385876\/kiag560.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plphys\/article-pdf\/202\/1\/kiag560\/70385876\/kiag560.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plphys\/article-pdf\/202\/1\/kiag560\/70385876\/kiag560.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:26:59Z","timestamp":1788546419000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plphys\/article\/doi\/10.1093\/plphys\/kiag560\/8747339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":94,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/plphys\/kiag560","relation":{},"ISSN":["0032-0889","1532-2548"],"issn-type":[{"value":"0032-0889","type":"print"},{"value":"1532-2548","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,7,30]]},"article-number":"kiag560"}}