{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:35:14Z","timestamp":1789594514699,"version":"build-2803163510"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"MICIU"},{"name":"ERDF A way of making Europe"},{"name":"CERCA Programme\/Generalitat de Catalunya","award":["2017SGR-718"],"award-info":[{"award-number":["2017SGR-718"]}]},{"name":"CERCA Programme\/Generalitat de Catalunya","award":["2021SGR-792"],"award-info":[{"award-number":["2021SGR-792"]}]},{"DOI":"10.13039\/501100003329","name":"Spanish Ministry of Economy and Competitiveness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2021-123500NB-I00"],"award-info":[{"award-number":["PID2021-123500NB-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["TED2021-130912B-I00"],"award-info":[{"award-number":["TED2021-130912B-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Sevilla"},{"name":"Juan de la Cierva Incorporacio\u0301n","award":["IJC2018\u2013035773"],"award-info":[{"award-number":["IJC2018\u2013035773"]}]},{"name":"Beatriu de Pino\u0301s","award":["2018 BP 0032"],"award-info":[{"award-number":["2018 BP 0032"]}]},{"name":"AGAUR-FI ajuts","award":["2020 FI_B_00343"],"award-info":[{"award-number":["2020 FI_B_00343"]}]},{"name":"Department of Research and Universities of the Generalitat of Catalonia"},{"DOI":"10.13039\/501100004895","name":"European Social Plus Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["CEX2019-000902-S"],"award-info":[{"award-number":["CEX2019-000902-S"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MICIU"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Understanding chloroplast biogenesis is key given the importance of photosynthesis and chloroplast metabolism. Chloroplast assembly has been extensively studied in angiosperms, where the main hallmarks have been established, including the need for nuclear and plastid genome coordination through anterograde and retrograde signals. However, recent works in other photosynthetic lineages have revealed both divergent and conserved regulators. These differences likely represent adaptive strategies that emerged during the evolution of the green lineage and highlight the need for evolutionary studies to fully elucidate the mechanisms controlling chloroplast biogenesis, a light-promoted process. Here, we characterize the yellow-in-the-dark chIL mutant of Chlamydomonas reinhardtii, which carries a mutation in the CHLL subunit of the dark-operative POR enzyme. Dark-adapted chIL cells cannot synthesize chlorophylls and display a de-differentiated chloroplast, and our results show that upon exposure to light, chloroplast biogenesis is initiated very rapidly based on starch reserves. We demonstrate that accumulation of pigments, photosynthesis-related proteins, and functional chloroplast activity is detected within 1 to 3\u2005h of illumination. Additionally, we show that lincomycin, an inhibitor of plastid translation, specifically affects chloroplast physiology, blocks light-induced chloroplast biogenesis and cell growth, and triggers retrograde signaling, establishing lincomycin as a valuable tool for investigating chloroplast physiology and plastid-to-nucleus communication in Chlamydomonas. Together, this study introduces the chIL mutant as a powerful model system to dissect light-induced, time-resolved chloroplast biogenesis, as well as the responses and signaling triggered by lincomycin. By promoting broader adoption of this model, we aim to help inform strategies to enhance photosynthesis and stress tolerance and optimize bioproduct production.<\/jats:p>","DOI":"10.1093\/plphys\/kiag600","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:19:12Z","timestamp":1786706352000},"source":"Crossref","is-referenced-by-count":1,"title":["The yellow-in-the-dark\n                    <i>chIL<\/i>\n                    Chlamydomonas mutant as a model for time-resolved chloroplast biogenesis and retrograde signaling"],"prefix":"10.1093","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2281-6700","authenticated-orcid":false,"given":"M \u00c1guila","family":"Ruiz-Sola","sequence":"first","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB, Bellaterra, Barcelona 08193 ,","place":["Spain"]},{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, Universidad de Sevilla-CSIC , Av. Am\u00e9rico Vespucio 49, Sevilla 41092 ,","place":["Spain"]},{"name":"Departamento de Bioqu\u00edmica Vegetal y Biolog\u00eda Molecular, Facultad de Qu\u00edmica, Universidad de Sevilla , Calle Profesor Garc\u00eda Gonz\u00e1lez 1, Sevilla 41012 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6455-8075","authenticated-orcid":false,"given":"Mariano A","family":"De Silvio","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB, Bellaterra, Barcelona 08193 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6060-5919","authenticated-orcid":false,"given":"Mario","family":"Becerra-Tinoco","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, Universidad de Sevilla-CSIC , Av. Am\u00e9rico Vespucio 49, Sevilla 41092 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6254-3187","authenticated-orcid":false,"given":"Andrea M","family":"Quintero-Moreno","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, Universidad de Sevilla-CSIC , Av. Am\u00e9rico Vespucio 49, Sevilla 41092 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7738-5002","authenticated-orcid":false,"given":"Beatrice","family":"Pacenza","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB, Bellaterra, Barcelona 08193 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3514-1025","authenticated-orcid":false,"given":"Jos\u00e9 L","family":"Crespo","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, Universidad de Sevilla-CSIC , Av. Am\u00e9rico Vespucio 49, Sevilla 41092 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7340-9355","authenticated-orcid":false,"given":"Elena","family":"Monte","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB, Bellaterra, Barcelona 08193 ,","place":["Spain"]},{"name":"Consejo Superior de Investigaciones Cient\u00edficas (CSIC) , Barcelona 08028 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"2026082713420447900_kiag600-B1","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S1011-1344(98)00063-3","article-title":"Greening in the dark: light-independent chlorophyll biosynthesis from anoxygenic photosynthetic bacteria to gymnosperms","volume":"43","author":"Armstrong","year":"1998","journal-title":"J Photochem Photobiol 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