{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:21:59Z","timestamp":1787854919294,"version":"build-2784847793"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370737"],"award-info":[{"award-number":["32370737"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Most plastid proteins are encoded by nuclear genes and synthesized in the cytosol as preproteins (or precursors). These preproteins consist of N-terminal transit peptides (TPs) followed by mature peptides (MPs, which ultimately fold into their mature conformations inside plastids). The preproteins are transported across plastid membranes via the translocon at the outer membrane of chloroplasts (TOC)\/translocon at the inner membrane of chloroplasts (TIC) complex. Since the channel of TOC\/TIC is too narrow to accommodate fully or highly folded preproteins, it has been widely assumed that preproteins are maintained in an unfolded or partly folded state in the cytosol to facilitate their import. However, whether a dedicated mechanism exists to actively inhibit MP folding in the cytosol remains unclear. Here, we show that such a mechanism may not exist, because MPs can acquire folded conformations while still in the cytosol. Surprisingly, we found that 2 preproteins with identical amino-acid sequences\u2014one produced by de novo synthesis and the other by protease-mediated cleavage\u2014exhibited different organelle-targeting abilities, suggesting that the primary structures of TPs are not sufficient to direct exclusive plastid import and that TPs need to fold into a specific conformation to function properly. Based on these results, we propose that the faster folding of TPs, relative to downstream MPs, plays an important role in maintaining preproteins in an import-competent state.<\/jats:p>","DOI":"10.1093\/plphys\/kiag619","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:50:16Z","timestamp":1787226616000},"source":"Crossref","is-referenced-by-count":0,"title":["Folding states of transit peptides and mature peptides in the cytosol: implications for the import-competent state of preproteins"],"prefix":"10.1093","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4411-8735","authenticated-orcid":false,"given":"Jiao-Jiao","family":"Shi","sequence":"first","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University , Xi\u2019an 710069 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