{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:04:37Z","timestamp":1790103877465,"version":"4.0.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fruit size and shape, which influence horticultural quality, are determined by the number and the size of the cells in the local region. In fruit trees, however, the difficulty of applying molecular genetic approaches has hindered a detailed understanding of the localization and orientation of cell division in developing fruit tissues. In this study, we established a novel framework to visualize cell division in pre-anthesis ovaries of drupe crops, providing clear insight into the spatial distribution and orientation of dividing cells. We systematically optimized a 5-ethynyl-2\u2032-deoxyuridine (EdU) labeling protocol for thick ovary tissues by adjusting infiltration conditions and fixation methods. In addition, electron microscopy combined with wide-view tiling visualization was applied to directly identify dividing cells, including those undergoing chromosome segregation and cell plate formation. By combining with machine learning-based detection, we efficiently and objectively identified dividing cells. Using these complementary approaches, we found that cell division activity was broadly distributed throughout pre-anthesis ovaries in several drupe crops analyzed, without pronounced spatial restriction. In contrast, analysis of division orientation revealed region-specific patterns: cells in the outermost exocarp divided predominantly anticlinally, whereas cells in the mesocarp divided largely periclinally, consistent with subsequent ovary (fruit) enlargement. The integrated framework presented here provides a foundation for understanding the spatial and three-dimensional regulation of cell division during pre-anthesis ovary development and may be extended in future studies to later stages of fruit development.<\/jats:p>","DOI":"10.1093\/plphys\/kiag711","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:36:40Z","timestamp":1790077000000},"source":"Crossref","is-referenced-by-count":0,"title":["A visualization framework for cell division activity and orientation in pre-anthesis ovaries of\n                    <i>Prunus<\/i>\n                    species"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3180-5389","authenticated-orcid":false,"given":"Ayame","family":"Shimbo","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3451-6166","authenticated-orcid":false,"given":"Soichiro","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8502 ,","place":["Japan"]},{"name":"Research Center for Agricultural Information Technology, National Agriculture and Food Research Organization , Tsukuba, Ibaraki 305-8517 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-4382","authenticated-orcid":false,"given":"Tatsuya","family":"Katsuno","sequence":"additional","affiliation":[{"name":"Center of Anatomical Studies, Graduate School of Medicine, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8501 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5183-6979","authenticated-orcid":false,"given":"Akane","family":"Kusumi","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-7863","authenticated-orcid":false,"given":"Hisayo","family":"Yamane","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4913-6278","authenticated-orcid":false,"given":"Masahiro M","family":"Kanaoka","sequence":"additional","affiliation":[{"name":"Faculty of Bioresource Sciences, Prefectural University of Hiroshima , Nanatsuka-Cho, Shobara, Hiroshima 727-0023 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7811-5789","authenticated-orcid":false,"given":"Ryutaro","family":"Tao","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto, Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,22]]},"container-title":["Plant Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/plphys\/advance-article-pdf\/doi\/10.1093\/plphys\/kiag711\/71319668\/kiag711.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plphys\/advance-article-pdf\/doi\/10.1093\/plphys\/kiag711\/71319668\/kiag711.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:34:04Z","timestamp":1790102044000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plphys\/advance-article\/doi\/10.1093\/plphys\/kiag711\/8828989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/plphys\/kiag711","relation":{},"ISSN":["0032-0889","1532-2548"],"issn-type":[{"value":"0032-0889","type":"print"},{"value":"1532-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"article-number":"kiag711"}}