{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:50:29Z","timestamp":1791571829524,"version":"4.3.4"},"reference-count":93,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007131","name":"Texas Tech University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007131","id-type":"DOI","asserted-by":"publisher"}]},{"name":"USDA-ARS CRIS","award":["3096-21000-023-00-D"],"award-info":[{"award-number":["3096-21000-023-00-D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sorghum (Sorghum bicolor), a C4 grass adapted to hot semi-arid environments, depends on reinforced xylem vessels to maintain hydraulic conductance under high evaporative demand. During xylem differentiation, coordinated microtubule and actin dynamics guide secondary cell wall (SCW) deposition; however, whether actin-based motors regulate vascular architecture and hydraulic performance has remained unknown. Here, we identify HEAT-SENSITIVE 1 (HS1), a previously uncharacterized myosin VIII, as a central regulator of protoxylem (PX) integrity and water transport in sorghum. The hs1 mutant exhibited severe leaf scorching under field conditions, accompanied by pronounced PX defects under controlled environments, including vessel collapse, reduced lumen area, and attenuated lignified SCWs. These structural abnormalities compromised longitudinal hydraulic conductance, diminished whole-plant water use, and rendered developing leaves unable to meet transpirational demand, resulting in transient water deficit and secondary tissue injury. HS1 encoded a grass-specific myosin VIII with a grass-specific N-terminal extension exhibiting high intrinsic disorder in N-terminal and lineage-specific substitutions in the motor domain. Single-cell transcriptome analyses positioned HS1 within differentiating PX cells of developing leaves, revealing pronounced temporal and cell type specificity. Furthermore, bulk transcriptome profiling and quantitative lignin measurements indicated that HS1 is required for proper lignin deposition during PX differentiation, linking an actin-based motor to wall reinforcement. Reduced nucleotide diversity at the HS1 locus further supports strong evolutionary constraint, suggesting an adaptive role in sorghum hydraulic resilience. Together, these findings establish HS1 as an actin-based motor controlling xylem architecture and hydraulic function in plants, a previously unrecognized component of grass hydraulic regulation not evident in Arabidopsis.<\/jats:p>","DOI":"10.1093\/plphys\/kiag712","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:36:40Z","timestamp":1790077000000},"source":"Crossref","is-referenced-by-count":0,"title":["A grass-specific structural myosin VIII regulates protoxylem development and hydraulic conductance in sorghum"],"prefix":"10.1093","volume":"202","author":[{"given":"Zhiyuan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University , 2500 Broadway, Lubbock, TX 79409 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University , 2500 Broadway, Lubbock, TX 79409 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8855-5521","authenticated-orcid":false,"given":"Leonidas","family":"D\u2019Agostino","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University , 2500 Broadway, Lubbock, TX 79409 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1471-7785","authenticated-orcid":false,"given":"Zhanguo","family":"Xin","sequence":"additional","affiliation":[{"name":"USDA-ARS, Cropping Systems Research Laboratory, Plant Stress and Germplasm Development Unit , 3810 4th Street, Lubbock, TX 79415 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9060-8017","authenticated-orcid":false,"given":"Junping","family":"Chen","sequence":"additional","affiliation":[{"name":"USDA-ARS, Cropping Systems Research Laboratory, Plant Stress and Germplasm Development Unit , 3810 4th Street, Lubbock, TX 79415 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5420-9884","authenticated-orcid":false,"given":"Gunvant Baliram","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University , 2500 Broadway, Lubbock, TX 79409 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6016-9639","authenticated-orcid":false,"given":"Yinping","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University , 2500 Broadway, Lubbock, TX 79409 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"2026100914015494400_kiag712-B1","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/tpj.15930","article-title":"Barley Ror1 encodes a class XI myosin required for mlo-based broad-spectrum resistance to the fungal powdery mildew pathogen","volume":"112","author":"Acevedo-Garcia","year":"2022","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B2","doi-asserted-by":"publisher","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B3","doi-asserted-by":"publisher","first-page":"1074","DOI":"10.1111\/tpj.14675","article-title":"A rapid and quantitative safranin-based fluorescent microscopy method to evaluate cell wall lignification","volume":"102","author":"Baldacci-Cresp","year":"2020","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B4","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1111\/tpj.15853","article-title":"Sorghum association panel whole-genome sequencing establishes cornerstone resource for dissecting genomic diversity","volume":"111","author":"Boatwright","year":"2022","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B5","doi-asserted-by":"publisher","first-page":"17555","DOI":"10.1073\/pnas.0408024101","article-title":"Evolution of xylem lignification and hydrogel transport regulation","volume":"101","author":"Boyce","year":"2004","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026100914015494400_kiag712-B6","doi-asserted-by":"publisher","first-page":"170","DOI":"10.3389\/fpls.2012.00170","article-title":"Are kinesins required for organelle trafficking in plant cells?","volume":"3","author":"Cai","year":"2012","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B7","doi-asserted-by":"publisher","first-page":"1718","DOI":"10.1104\/pp.113.228379","article-title":"A hydraulic model is compatible with rapid changes in leaf elongation under fluctuating evaporative demand and soil water status","volume":"164","author":"Caldeira","year":"2014","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B8","doi-asserted-by":"publisher","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: an integrative toolkit developed for interactive analyses of big biological data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol Plant."},{"key":"2026100914015494400_kiag712-B9","doi-asserted-by":"publisher","first-page":"7565","DOI":"10.1038\/s41467-025-62844-1","article-title":"Fibrous network nature of plant cell walls enables tunable mechanics for development","volume":"16","author":"Chen","year":"2025","journal-title":"Nat Commun."},{"key":"2026100914015494400_kiag712-B10","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1111\/j.1365-313X.2005.02452.x","article-title":"Disruption of the cellulose synthase gene, AtCesA8\/IRX1, enhances drought and osmotic stress tolerance in Arabidopsis","volume":"43","author":"Chen","year":"2005","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B11","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1007\/s40502-025-00912-w","article-title":"Single-cell transcriptomic landscape of sorghum leaf reveals cell-specific mineral nutrient regulation","volume":"30","author":"D\u2019Agostino","year":"2025","journal-title":"Plant Physiol Rep"},{"key":"2026100914015494400_kiag712-B12","doi-asserted-by":"publisher","first-page":"20908","DOI":"10.1073\/pnas.2005014117","article-title":"Necrotic upper tips1 mimics heat and drought stress and encodes a protoxylem-specific transcription factor in maize","volume":"117","author":"Dong","year":"2020","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026100914015494400_kiag712-B13","doi-asserted-by":"publisher","first-page":"571072","DOI":"10.3389\/fpls.2021.571072","article-title":"Drought-induced root pressure in Sorghum bicolor","volume":"12","author":"Drobnitch","year":"2021","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated profile HMM searches","volume":"7","author":"Eddy","year":"2011","journal-title":"PLoS Comput Biol."},{"key":"2026100914015494400_kiag712-B15","doi-asserted-by":"publisher","first-page":"1665967","DOI":"10.3389\/fpls.2025.1665967","article-title":"Sorghum as a monocot model for drought research","volume":"16","author":"Fontanet-Manzaneque","year":"2025","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B16","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1104\/pp.010514","article-title":"The Arabidopsis xylem peptidase XCP1 is a tracheary element vacuolar protein that may be a papain ortholog","volume":"128","author":"Funk","year":"2002","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B17","doi-asserted-by":"publisher","first-page":"54","DOI":"10.11648\/j.abb.20231103.14","article-title":"Sorghum as a model crop for drought stress tolerance","volume":"11","author":"Gidi","year":"2023","journal-title":"Adv Biosci Bioeng"},{"key":"2026100914015494400_kiag712-B18","doi-asserted-by":"publisher","first-page":"D1178","DOI":"10.1093\/nar\/gkr944","article-title":"Phytozome: a comparative platform for green plant genomics","volume":"40","author":"Goodstein","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B19","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1002\/j.1537-2197.1942.tb10286.x","article-title":"On the development of xylary elements in the first internode of Avena in dark and light","volume":"29","author":"Goodwin","year":"1942","journal-title":"Am J Bot"},{"key":"2026100914015494400_kiag712-B20","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1104\/pp.119.2.375","article-title":"Tracheary element differentiation uses a novel mechanism coordinating programmed cell death and secondary cell wall synthesis","volume":"119","author":"Groover","year":"1999","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B21","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"2026100914015494400_kiag712-B22","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1038\/s41477-023-01593-9","article-title":"Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana","volume":"10","author":"Higa","year":"2024","journal-title":"Nat Plants."},{"key":"2026100914015494400_kiag712-B23","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1021\/acs.jcim.7b00397","article-title":"ATPbind: accurate protein\u2013ATP binding site prediction by combining sequence-profiling and structure-based comparisons","volume":"58","author":"Hu","year":"2018","journal-title":"J Chem Inf Model."},{"key":"2026100914015494400_kiag712-B24","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1093\/plphys\/kiae329","article-title":"Tubulin participates in establishing protoxylem vessel reinforcement patterns and hydraulic conductivity in maize","volume":"196","author":"Huang","year":"2024","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B25","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1023\/A:1021236307508","article-title":"The plant cytoskeleton: recent advances in the study of the plant microtubule-associated proteins MAP-65, MAP-190 and the Xenopus MAP215-like protein, MOR1","volume":"50","author":"Hussey","year":"2002","journal-title":"Plant Mol Biol."},{"key":"2026100914015494400_kiag712-B26","doi-asserted-by":"publisher","first-page":"941","DOI":"10.3389\/fpls.2016.00941","article-title":"Vulnerability of protoxylem and metaxylem vessels to embolisms and radial refilling in a vascular bundle of maize leaves","volume":"7","author":"Hwang","year":"2016","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B27","doi-asserted-by":"publisher","first-page":"1551","DOI":"10.1105\/tpc.16.00373","article-title":"A sorghum mutant resource as an efficient platform for gene discovery in grasses","volume":"28","author":"Jiao","year":"2016","journal-title":"Plant Cell"},{"key":"2026100914015494400_kiag712-B28","doi-asserted-by":"publisher","first-page":"2267","DOI":"10.3389\/fpls.2017.02267","article-title":"Efficient identification of causal mutations through sequencing of bulked F 2 from two allelic bloomless mutants of Sorghum bicolor","volume":"8","author":"Jiao","year":"2018","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B29","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1111\/tpj.16582","article-title":"A large sequenced mutant library\u2013valuable reverse genetic resource that covers 98% of sorghum genes","volume":"117","author":"Jiao","year":"2024","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B30","doi-asserted-by":"publisher","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"2026100914015494400_kiag712-B31","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1093\/aob\/mci130","article-title":"Biomechanical model of the xylem vessels in vascular plants","volume":"95","author":"Karam","year":"2005","journal-title":"Ann Bot."},{"key":"2026100914015494400_kiag712-B32","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1939-8433-6-4","article-title":"Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data","volume":"6","author":"Kawahara","year":"2013","journal-title":"Rice"},{"key":"2026100914015494400_kiag712-B33","doi-asserted-by":"publisher","first-page":"1921","DOI":"10.1038\/s41467-025-56866-y","article-title":"Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels","volume":"16","author":"Kijima","year":"2025","journal-title":"Nat Commun."},{"key":"2026100914015494400_kiag712-B34","doi-asserted-by":"publisher","first-page":"bar030","DOI":"10.1093\/database\/bar030","article-title":"Ensembl BioMarts: a hub for data retrieval across taxonomic space","volume":"2011","author":"Kinsella","year":"2011","journal-title":"Database"},{"key":"2026100914015494400_kiag712-B35","doi-asserted-by":"publisher","first-page":"1855","DOI":"10.1101\/gad.1331305","article-title":"Transcription switches for protoxylem and metaxylem vessel formation","volume":"19","author":"Kubo","year":"2005","journal-title":"Genes Dev."},{"key":"2026100914015494400_kiag712-B36","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1111\/j.1365-313X.2006.02918.x","article-title":"The Arabidopsis cinnamoyl CoA reductase irx4 mutant has a delayed but coherent (normal) program of lignification","volume":"48","author":"Laskar","year":"2006","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B37","doi-asserted-by":"publisher","DOI":"10.1002\/pld3.498","article-title":"Plant height shapes hydraulic architecture but does not predict metaxylem area under drought in Sorghum bicolor","volume":"7","author":"Lehrer","year":"2023","journal-title":"Plant Direct"},{"key":"2026100914015494400_kiag712-B38","doi-asserted-by":"publisher","first-page":"1834","DOI":"10.1093\/plphys\/kiad390","article-title":"IQ domain-containing protein ZmIQD27 modulates water transport in maize","volume":"193","author":"Li","year":"2023","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B39","doi-asserted-by":"publisher","first-page":"101616","DOI":"10.1016\/j.xplc.2025.101616","article-title":"Myosin VIII and XI isoforms interact with Agrobacterium VirE2 protein and help direct transport from the plasma membrane to the perinuclear region during plant transformation","volume":"7","author":"Liu","year":"2026","journal-title":"Plant Commun"},{"key":"2026100914015494400_kiag712-B40","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1186\/s13068-021-02016-7","article-title":"SorGSD: updating and expanding the sorghum genome science database with new contents and tools","volume":"14","author":"Liu","year":"2021","journal-title":"Biotechnol Biofuels."},{"key":"2026100914015494400_kiag712-B41","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1016\/j.pbi.2013.10.004","article-title":"Understanding myosin functions in plants: are we there yet?","volume":"16","author":"Madison","year":"2013","journal-title":"Curr Opin Plant Biol."},{"key":"2026100914015494400_kiag712-B42","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1046\/j.1469-8137.2000.00641.x","article-title":"Changes in axial hydraulic conductivity along elongating leaf blades in relation to xylem maturation in tall fescue","volume":"146","author":"Martre","year":"2000","journal-title":"New Phytol."},{"key":"2026100914015494400_kiag712-B43","doi-asserted-by":"publisher","first-page":"W329","DOI":"10.1093\/nar\/gky384","article-title":"IUPred2A: context-dependent prediction of protein disorder as a function of redox state and protein binding","volume":"46","author":"M\u00e9sz\u00e1ros","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B44","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1073\/pnas.1215985110","article-title":"Population genomic and genome-wide association studies of agroclimatic traits in sorghum","volume":"110","author":"Morris","year":"2013","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026100914015494400_kiag712-B45","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1186\/1471-2148-13-202","article-title":"Whole genome duplication events in plant evolution reconstructed and predicted using myosin motor proteins","volume":"13","author":"M\u00fchlhausen","year":"2013","journal-title":"BMC Evol Biol."},{"key":"2026100914015494400_kiag712-B46","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1146\/annurev-arplant-042817-040024","article-title":"Kinesins and myosins: molecular motors that coordinate cellular functions in plants","volume":"69","author":"Nebenf\u00fchr","year":"2018","journal-title":"Annu Rev Plant Biol."},{"key":"2026100914015494400_kiag712-B47","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.17244","article-title":"Emerging frontiers in sorghum genetic engineering","volume":"121","author":"Nigam","year":"2025","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B48","doi-asserted-by":"publisher","first-page":"4439","DOI":"10.1105\/tpc.113.117853","article-title":"Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns","volume":"25","author":"Oda","year":"2013","journal-title":"Plant Cell."},{"key":"2026100914015494400_kiag712-B49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/nplants.2015.31","article-title":"Regulation of organ straightening and plant posture by an actin\u2013myosin XI cytoskeleton","volume":"1","author":"Okamoto","year":"2015","journal-title":"Nat Plants."},{"key":"2026100914015494400_kiag712-B50","doi-asserted-by":"publisher","first-page":"dev201762","DOI":"10.1242\/dev.201762","article-title":"The class VIII myosin ATM1 is required for root apical meristem function","volume":"150","author":"Olatunji","year":"2023","journal-title":"Development"},{"key":"2026100914015494400_kiag712-B51","doi-asserted-by":"publisher","first-page":"D246","DOI":"10.1093\/nar\/gkx1158","article-title":"Expression Atlas: gene and protein expression across multiple studies and organisms","volume":"46","author":"Papatheodorou","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B52","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1111\/j.1469-8137.2012.04086.x","article-title":"Temperature responses of developmental processes have not been affected by breeding in different ecological areas for 17 crop species","volume":"194","author":"Parent","year":"2012","journal-title":"New Phytol."},{"key":"2026100914015494400_kiag712-B53","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1104\/pp.110.170720","article-title":"Expression, splicing, and evolution of the myosin gene family in plants","volume":"155","author":"Peremyslov","year":"2011","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B54","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1093\/bioinformatics\/btab667","article-title":"ATPdock: a template-based method for ATP-specific protein\u2013ligand docking","volume":"38","author":"Rao","year":"2022","journal-title":"Bioinformatics"},{"key":"2026100914015494400_kiag712-B55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/gb-2001-2-7-research0024","article-title":"Analysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence","volume":"2","author":"Reddy","year":"2001","journal-title":"Genome Biol."},{"key":"2026100914015494400_kiag712-B56","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1471-2164-2-2","article-title":"Kinesins in the Arabidopsis genome: a comparative analysis among eukaryotes","volume":"2","author":"Reddy","year":"2001","journal-title":"BMC Genomics"},{"key":"2026100914015494400_kiag712-B57","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1046\/j.1365-313X.1999.00553.x","article-title":"Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall","volume":"19","author":"Reichelt","year":"1999","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B58","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1038\/nature03949","article-title":"Myosin domain evolution and the primary divergence of eukaryotes","volume":"436","author":"Richards","year":"2005","journal-title":"Nature"},{"key":"2026100914015494400_kiag712-B59","doi-asserted-by":"publisher","first-page":"8930","DOI":"10.1073\/pnas.0403002101","article-title":"Myosin subfragment 1 structures reveal a partially bound nucleotide and a complex salt bridge that helps couple nucleotide and actin binding","volume":"101","author":"Risal","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026100914015494400_kiag712-B60","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1111\/j.1469-8137.2004.01143.x","article-title":"The genetic control of lignin deposition during plant growth and development","volume":"164","author":"Rogers","year":"2004","journal-title":"New Phytol."},{"key":"2026100914015494400_kiag712-B61","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1104\/pp.17.01429","article-title":"Update on myosin motors: molecular mechanisms and physiological functions","volume":"176","author":"Ryan","year":"2018","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B62","doi-asserted-by":"publisher","first-page":"e6","DOI":"10.1093\/pcp\/pcs183","article-title":"Rice Annotation Project Database (RAP-DB): an integrative and interactive database for rice genomics","volume":"54","author":"Sakai","year":"2013","journal-title":"Plant Cell Physiol."},{"key":"2026100914015494400_kiag712-B63","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat Methods."},{"key":"2026100914015494400_kiag712-B64","volume-title":"The PyMOL molecular graphics system, version 1.8","author":"Schrodinger","year":"2015"},{"key":"2026100914015494400_kiag712-B65","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1111\/nph.14256","article-title":"Leaf vein xylem conduit diameter influences susceptibility to embolism and hydraulic decline","volume":"213","author":"Scoffoni","year":"2017","journal-title":"New Phytol."},{"key":"2026100914015494400_kiag712-B66","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1093\/gbe\/evu013","article-title":"Evolution and classification of myosins, a paneukaryotic whole-genome approach","volume":"6","author":"Sebe-Pedros","year":"2014","journal-title":"Genome Biol Evol."},{"key":"2026100914015494400_kiag712-B67","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1093\/oxfordjournals.aob.a088407","article-title":"Developmental anatomy of the shoot of Zea mays L","volume":"6","author":"Sharman","year":"1942","journal-title":"Ann Bot."},{"key":"2026100914015494400_kiag712-B68","doi-asserted-by":"publisher","first-page":"D451","DOI":"10.1093\/nar\/gkaf1188","article-title":"The PROSITE database for protein families, domains, and sites","volume":"54","author":"Sigrist","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B69","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S1369-5266(02)00012-2","article-title":"Cytoskeletal control of plant cell shape: getting the fine points","volume":"6","author":"Smith","year":"2003","journal-title":"Curr Opin Plant Biol."},{"key":"2026100914015494400_kiag712-B70","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1093\/mp\/ssr063","article-title":"Recent advances in understanding plant myosin function: life in the fast lane","volume":"4","author":"Sparkes","year":"2011","journal-title":"Mol Plant."},{"key":"2026100914015494400_kiag712-B71","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/s41586-024-08204-3","article-title":"Exaptation of ancestral cell-identity networks enables C4 photosynthesis","volume":"636","author":"Swift","year":"2024","journal-title":"Nature"},{"key":"2026100914015494400_kiag712-B72","doi-asserted-by":"publisher","first-page":"2313","DOI":"10.1093\/jxb\/erae031","article-title":"Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins","volume":"75","author":"Symonds","year":"2024","journal-title":"J Exp Bot."},{"key":"2026100914015494400_kiag712-B73","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1023\/B:CELL.0000046405.11326.a8","article-title":"Cellulose synthesis in the Arabidopsis secondary cell wall","volume":"11","author":"Taylor","year":"2004","journal-title":"Cellulose"},{"key":"2026100914015494400_kiag712-B74","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1111\/nph.19463","article-title":"Plant hydraulics at the heart of plant, crops and ecosystem functions in the face of climate change","volume":"241","author":"Torres-Ruiz","year":"2024","journal-title":"New Phytol."},{"key":"2026100914015494400_kiag712-B75","doi-asserted-by":"publisher","first-page":"689","DOI":"10.2307\/3870425","article-title":"Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall","volume":"9","author":"Turner","year":"1997","journal-title":"Plant Cell."},{"key":"2026100914015494400_kiag712-B76","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1104\/pp.110.155119","article-title":"Lignin biosynthesis and structure","volume":"153","author":"Vanholme","year":"2010","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B77","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1111\/j.1365-3040.2010.02270.x","article-title":"Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival","volume":"34","author":"Voelker","year":"2011","journal-title":"Plant Cell Environ."},{"key":"2026100914015494400_kiag712-B78","doi-asserted-by":"publisher","first-page":"3447","DOI":"10.1105\/tpc.112.101360","article-title":"Opaque1 encodes a myosin XI motor protein that is required for endoplasmic reticulum motility and protein body formation in maize endosperm","volume":"24","author":"Wang","year":"2012","journal-title":"Plant Cell."},{"key":"2026100914015494400_kiag712-B79","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/jxb\/ert437","article-title":"Genome-wide identification, splicing, and expression analysis of the myosin gene family in maize (Zea mays)","volume":"65","author":"Wang","year":"2014","journal-title":"J Exp Bot."},{"key":"2026100914015494400_kiag712-B80","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview version 2\u2014a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"2026100914015494400_kiag712-B81","doi-asserted-by":"publisher","first-page":"718","DOI":"10.1111\/j.1365-313X.2008.03724.x","article-title":"The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation","volume":"57","author":"Wu","year":"2009","journal-title":"Plant J."},{"key":"2026100914015494400_kiag712-B82","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03498","article-title":"Myosin VIII associates with microtubule ends and together with actin plays a role in guiding plant cell division","volume":"3","author":"Wu","year":"2014","journal-title":"Elife"},{"key":"2026100914015494400_kiag712-B83","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1093\/mp\/ssr068","article-title":"Myosin VIII regulates protonemal patterning and developmental timing in the moss Physcomitrella patens","volume":"4","author":"Wu","year":"2011","journal-title":"Mol Plant."},{"key":"2026100914015494400_kiag712-B84","doi-asserted-by":"publisher","first-page":"3560","DOI":"10.3390\/ijms22073560","article-title":"MYB transcription factors and its regulation in secondary cell wall formation and lignin biosynthesis during xylem development","volume":"22","author":"Xiao","year":"2021","journal-title":"Int J Mol Sci."},{"key":"2026100914015494400_kiag712-B85","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1186\/1471-2229-8-103","article-title":"Applying genotyping (TILLING) and phenotyping analyses to elucidate gene function in a chemically induced sorghum mutant population","volume":"8","author":"Xin","year":"2008","journal-title":"BMC Plant Biol."},{"key":"2026100914015494400_kiag712-B86","doi-asserted-by":"publisher","first-page":"237","DOI":"10.5511\/plantbiotechnology.27.237","article-title":"Transcriptional regulation of secondary wall formation controlled by NAC domain proteins","volume":"27","author":"Yamaguchi","year":"2010","journal-title":"Plant Biotechnol"},{"key":"2026100914015494400_kiag712-B87","doi-asserted-by":"publisher","first-page":"D996","DOI":"10.1093\/nar\/gkab1007","article-title":"Ensembl genomes 2022: an expanding genome resource for non-vertebrates","volume":"50","author":"Yates","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2026100914015494400_kiag712-B88","doi-asserted-by":"publisher","first-page":"1706603","DOI":"10.3389\/fpls.2025.1706603","article-title":"Integrated morphological, physiological, and transcriptomic analyses uncover the mechanisms of waterlogging tolerance in Sorghum bicolor (L.)","volume":"16","author":"Zhang","year":"2025","journal-title":"Front Plant Sci."},{"key":"2026100914015494400_kiag712-B89","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.1104\/pp.19.00018","article-title":"Myosins XI are involved in exocytosis of cellulose synthase complexes","volume":"179","author":"Zhang","year":"2019","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B90","doi-asserted-by":"publisher","first-page":"2539","DOI":"10.1104\/pp.15.01011","article-title":"MicroRNA857 is involved in the regulation of secondary growth of vascular tissues in Arabidopsis","volume":"169","author":"Zhao","year":"2015","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B91","doi-asserted-by":"publisher","first-page":"2776","DOI":"10.1105\/tpc.107.053678","article-title":"The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis","volume":"19","author":"Zhong","year":"2007","journal-title":"Plant Cell."},{"key":"2026100914015494400_kiag712-B92","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1104\/pp.114.251462","article-title":"The fragile Fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components","volume":"167","author":"Zhu","year":"2015","journal-title":"Plant Physiol."},{"key":"2026100914015494400_kiag712-B93","doi-asserted-by":"publisher","first-page":"2816","DOI":"10.1038\/s41467-026-69436-7","article-title":"NUT1-Exo70A1 regulates xylem vessel development and influences water use efficiency in maize","volume":"7","author":"Zhu","year":"2026","journal-title":"Nat Commun."}],"container-title":["Plant Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/plphys\/advance-article-pdf\/doi\/10.1093\/plphys\/kiag712\/71319673\/kiag712.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plphys\/article-pdf\/202\/2\/kiag712\/71319673\/kiag712.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/plphys\/article-pdf\/202\/2\/kiag712\/71319673\/kiag712.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:06:57Z","timestamp":1791569217000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plphys\/article\/doi\/10.1093\/plphys\/kiag712\/8828990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":93,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1093\/plphys\/kiag712","relation":{},"ISSN":["0032-0889","1532-2548"],"issn-type":[{"value":"0032-0889","type":"print"},{"value":"1532-2548","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,10]]},"published":{"date-parts":[[2026,9,22]]},"article-number":"kiag712"}}