{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T10:37:53Z","timestamp":1770460673170,"version":"3.49.0"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["HR0011-16-2\u20130036"],"award-info":[{"award-number":["HR0011-16-2\u20130036"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Health under Vector Biology","award":["1R01AI148787-01A1"],"award-info":[{"award-number":["1R01AI148787-01A1"]}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Promising genetics-based approaches are being developed to reduce or prevent the transmission of mosquito-vectored diseases. Less clear is how such transgenes can be removed from the environment, a concern that is particularly relevant for highly invasive gene drive transgenes. Here, we lay the groundwork for a transgene removal system based on single-strand annealing (SSA), a eukaryotic DNA repair mechanism. An SSA-based rescuer strain (kmoRG) was engineered to have direct repeat sequences (DRs) in the Aedes aegypti kynurenine 3-monooxygenase (kmo) gene flanking the intervening transgenic cargo genes, DsRED and EGFP. Targeted induction of DNA double-strand breaks (DSBs) in the DsRED transgene successfully triggered complete elimination of the entire cargo from the kmoRG strain, restoring the wild-type kmo gene, and thereby, normal eye pigmentation. Our work establishes the framework for strategies to remove transgene sequences during the evaluation and testing of modified strains for genetics-based mosquito control.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgac037","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T14:28:53Z","timestamp":1648650533000},"source":"Crossref","is-referenced-by-count":16,"title":["Engineering a self-eliminating transgene in the yellow fever mosquito,<i>Aedes aegypti<\/i>"],"prefix":"10.1093","volume":"1","author":[{"given":"Keun","family":"Chae","sequence":"first","affiliation":[{"name":"Department of Entomology, Texas A&M University , College Station, TX 77843 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanell","family":"Dawson","sequence":"additional","affiliation":[{"name":"Department of Entomology, Texas A&M University , College Station, TX 77843 , 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