{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T11:07:20Z","timestamp":1788606440948,"version":"build-2803163510"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","award":["AID-OAA-F-16\u201300082"],"award-info":[{"award-number":["AID-OAA-F-16\u201300082"]}],"id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["YUC2017-03-01-556"],"award-info":[{"award-number":["YUC2017-03-01-556"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The ability of the maternally transmitted endosymbiotic bacterium Wolbachia to induce cytoplasmic incompatibility (CI) and virus blocking makes it a promising weapon for combatting mosquito-borne diseases through either suppression or replacement of wild-type populations. Recent field trials show that both approaches significantly reduce the incidence of dengue fever in humans. However, new questions emerge about how Wolbachia-mosquito associations will co-evolve over time and whether Wolbachia-mediated virus blocking will be affected by the genetic diversity of mosquitoes and arboviruses in the real world. Here, we have compared the Wolbachia density and CI expression of two wAlbB-infected Aedes aegypti lines transinfected 15 years apart. We have also assessed wAlbB-mediated virus blocking against dengue (DENV), Zika (ZIKV), and Chikungunya (CHIKV) viruses and examined whether host genetic backgrounds modulate viral blocking effects by comparing ZIKV infection in mosquitoes with a Mexican genetic background to those with a Singaporean background. Our results show that over 15 years, wAlbB maintained the capacity to form a stable association with Ae. aegypti in terms of both density and CI expression. There were variations in wAlbB-induced virus blocking against CHIKV, DENV, and ZIKV, and higher inhibitory effects on ZIKV in mosquitoes on the Singaporean genetic background than on the Mexican background. These results provide important information concerning the robustness and long-term stability of Wolbachia as a biocontrol agent for arbovirus disease control.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgac203","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T04:24:12Z","timestamp":1663907052000},"source":"Crossref","is-referenced-by-count":37,"title":["<i>Wolbachia w<\/i>AlbB remains stable in <i>Aedes aegypti<\/i> over 15 years but exhibits genetic background-dependent variation in virus blocking"],"prefix":"10.1093","volume":"1","author":[{"given":"Xiao","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheong Huat","family":"Tan","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency , Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meichun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei Sze Jeslyn","family":"Wong","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency , Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meizhi Irene","family":"Li","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency , Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng Wai","family":"Mak","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency , Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdiel","family":"Mart\u00edn-Park","sequence":"additional","affiliation":[{"name":"Laboratorio para el Control Biologico de Aedes aegypti (LCB-UADY), Unidad Colaborativa para Bioensayos Entomologicos, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan , M\u00e9rida, Yucat\u00e1n CP 97315 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamili","family":"Contreras-Perera","sequence":"additional","affiliation":[{"name":"Laboratorio para el Control Biologico de Aedes aegypti (LCB-UADY), Unidad Colaborativa para Bioensayos Entomologicos, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan , M\u00e9rida, Yucat\u00e1n CP 97315 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Puerta-Guardo","sequence":"additional","affiliation":[{"name":"Laboratorio para el Control Biologico de Aedes aegypti (LCB-UADY), Unidad Colaborativa para Bioensayos Entomologicos, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan , M\u00e9rida, Yucat\u00e1n CP 97315 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Manrique-Saide","sequence":"additional","affiliation":[{"name":"Laboratorio para el Control Biologico de Aedes aegypti (LCB-UADY), Unidad Colaborativa para Bioensayos Entomologicos, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan , M\u00e9rida, Yucat\u00e1n CP 97315 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Ching","family":"Ng","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency , Singapore 138667"},{"name":"School of Biological Sciences, Nanyang Technological Institute , Singapore 637551"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-012X","authenticated-orcid":false,"given":"Zhiyong","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,22]]},"reference":[{"key":"2023012507023589400_bib1","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1038\/s41564-019-0476-8","article-title":"The current and future global distribution and population at risk of dengue","volume":"4","author":"Messina","year":"2019","journal-title":"Nat Microbiol"},{"key":"2023012507023589400_bib2","article-title":"Global strategy for dengue prevention and control 2012\u20132020","author":"Organization 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