{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:17:06Z","timestamp":1792621026897,"version":"4.5.0"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"vor","delay-in-days":46,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY030917"],"award-info":[{"award-number":["R01EY030917"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY026104"],"award-info":[{"award-number":["R01EY026104"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The cornea is a protective and refractive barrier in the eye crucial for vision. Understanding the human cornea in health, disease, and cell-based treatments can be greatly advanced with cornea organoids developed in culture from induced pluripotent stem cells. While a limited number of studies have investigated the single-cell transcriptomic composition of the human cornea, its organoids have not been examined similarly. Here, we elucidated the transcriptomic cell fate map of 4-month-old human cornea organoids and human donor corneas. The organoids harbor cell clusters that resemble cells of the corneal epithelium, stroma, and endothelium, with subpopulations that capture signatures of early developmental states. Unlike the adult cornea where the largest cell population is stromal, the organoids contain large proportions of epithelial and endothelial-like cells. These corneal organoids offer a 3D model to study corneal diseases and integrated responses of different cell types.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgac246","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:32:22Z","timestamp":1667003542000},"source":"Crossref","is-referenced-by-count":39,"title":["Single cell RNA-seq of human cornea organoids identifies cell fates of a developing immature cornea"],"prefix":"10.1093","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-5499","authenticated-orcid":false,"given":"George","family":"Maiti","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maith\u00ea Rocha","family":"Monteiro\u00a0de\u00a0Barros","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Dolgalev","sequence":"additional","affiliation":[{"name":"Applied Bioinformatics Laboratories, NYU Grossman School of Medicine , Science Building, Eighth Floor, 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4068-7700","authenticated-orcid":false,"given":"Mona","family":"Roshan","sequence":"additional","affiliation":[{"name":"University of California San Diego , ACTRI Building Rm Lower level 3E419, 9452 Medical Center Drive, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W","family":"Foster","sequence":"additional","affiliation":[{"name":"Wilmer Eye Institute, Johns Hopkins school of Medicine , Smith M037, 400 Broadway, Baltimore, MD 21287 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aristotelis","family":"Tsirigos","sequence":"additional","affiliation":[{"name":"Applied Bioinformatics Laboratories, NYU Grossman School of Medicine , Science Building, Eighth Floor, 435 E 30th , New York, NY 10016, USA"},{"name":"Department of Pathology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl J","family":"Wahlin","sequence":"additional","affiliation":[{"name":"University of California San Diego , ACTRI Building Rm Lower level 3E419, 9452 Medical Center Drive, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7543-1903","authenticated-orcid":false,"given":"Shukti","family":"Chakravarti","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"},{"name":"Department of Pathology, NYU Grossman School of Medicine , Science Building, Fifth Floor 435 E 30th , New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"2022121308593170900_bib1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1113\/jphysiol.1957.sp005758","article-title":"The structure and transparency of the cornea","volume":"136","author":"Maurice","year":"1957","journal-title":"J Physiol"},{"key":"2022121308593170900_bib2","article-title":"World report on 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