{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:25:00Z","timestamp":1788441900452,"version":"build-2803163510"},"reference-count":93,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T00:00:00Z","timestamp":1693612800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Agriculture and Food Research Initiative Competitive","award":["2018-67015-31936"],"award-info":[{"award-number":["2018-67015-31936"]}]},{"DOI":"10.13039\/100000199","name":"USDA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pregnancy loss is a significant problem when embryos produced in vitro are transferred to a synchronized uterus. Currently, mechanisms that underlie losses of in vitro\u2013produced embryos during implantation are largely unknown. We investigated this problem using cattle as a model of conceptus attachment by analyzing transcriptome data of paired extraembryonic membrane and endometrial samples collected on gestation days 18 and 25, which spans the attachment window in cattle. We identified that the transfer of an in vitro\u2013produced embryo caused a significant alteration in transcript abundance of hundreds of genes in extraembryonic and endometrial tissues on gestation days 18 and 25, when compared to pregnancies initiated by artificial insemination. Many of the genes with altered transcript abundance are associated with biological processes that are relevant to the establishment of pregnancy. An integrative analysis of transcriptome data from the conceptus and endometrium identified hundreds of putative ligand\u2013receptor pairs. There was a limited variation of ligand\u2013receptor pairs in pregnancies initiated by in vitro\u2013produced embryos on gestation day 18, and no alteration was observed on gestation day 25. In parallel, we identified that in vitro production of embryos caused an extensive alteration in the coexpression of genes expressed in the extraembryonic membranes and the corresponding endometrium on both gestation days. Both the transcriptional dysregulation that exists in the conceptus or endometrium independently and the rewiring of gene transcription between the conceptus and endometrium are a potential component of the mechanisms that contribute to pregnancy losses caused by in vitro production of embryos.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgad284","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T14:02:41Z","timestamp":1693663361000},"source":"Crossref","is-referenced-by-count":9,"title":["Extensive rewiring of the gene regulatory interactions between in vitro\u2013produced conceptuses and endometrium during attachment"],"prefix":"10.1093","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7895-0223","authenticated-orcid":false,"given":"Fernando H","family":"Biase","sequence":"first","affiliation":[{"name":"School of Animal Sciences, Virginia Polytechnic Institute and State University , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-3863","authenticated-orcid":false,"given":"Sarah E","family":"Moorey","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Tennessee , Knoxville, TN 37996 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1851-634X","authenticated-orcid":false,"given":"Julie G","family":"Schnuelle","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Auburn University , Auburn, AL 36849 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soren","family":"Rodning","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Auburn University , Auburn, AL 36849 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-7314","authenticated-orcid":false,"given":"Marta Sofia","family":"Ortega","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin Madison , Madison, WI 53706 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2815-766X","authenticated-orcid":false,"given":"Thomas E","family":"Spencer","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,2]]},"reference":[{"key":"2023091314114801600_pgad284-B1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1006\/dbio.2000.9767","article-title":"Embryo implantation","volume":"223","author":"Carson","year":"2000","journal-title":"Dev Biol"},{"key":"2023091314114801600_pgad284-B2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF00048682","article-title":"Multinomial logistic-regression 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