{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:31:10Z","timestamp":1790775070045,"version":"4.1.0"},"reference-count":70,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":26,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Habitability of Martian Environments","award":["339231"],"award-info":[{"award-number":["339231"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20\u2005m. In the upper 80\u2005cm of the playa sediments, microbial communities were dominated by Firmicutes taxa showing a depth-related decrease in biomass correlating with increasing amounts of soluble salts. High salt concentrations are possibly causing microbial colonization to cease in the lower part of the playa sediments between 80 and 200\u2005cm depth. In the underlying alluvial fan deposits, microbial communities reemerge, possibly due to gypsum providing an alternative water source. The discovery of this deeper subsurface community is reshaping our understanding of desert soils, emphasizing the need to consider subsurface environments in future explorations of arid ecosystems.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgae123","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T15:03:36Z","timestamp":1713884616000},"source":"Crossref","is-referenced-by-count":20,"title":["Persistent microbial communities in hyperarid subsurface habitats of the Atacama Desert: Insights from intracellular DNA analysis"],"prefix":"10.1093","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1750-3215","authenticated-orcid":false,"given":"Lucas","family":"Horstmann","sequence":"first","affiliation":[{"name":"GFZ German Research Centre for Geosciences , Section Geomicrobiology, 14473 Potsdam , Germany"},{"name":"Department Experimental Phycology and Culture Collection of Algae (EPSAG), Albrecht-von-Haller-Institute for Plant Sciences, Georg August University G\u00f6ttingen , 37073 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-9143","authenticated-orcid":false,"given":"Daniel","family":"Lipus","sequence":"additional","affiliation":[{"name":"GFZ German Research Centre for Geosciences , Section Geomicrobiology, 14473 Potsdam , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-7304","authenticated-orcid":false,"given":"Alexander","family":"Bartholom\u00e4us","sequence":"additional","affiliation":[{"name":"GFZ German Research Centre for Geosciences , Section Geomicrobiology, 14473 Potsdam , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9038-2476","authenticated-orcid":false,"given":"Felix","family":"Arens","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Astronomie und Astrophysik, Technische Universit\u00e4t Berlin , 10623 Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Airo","sequence":"additional","affiliation":[{"name":"Museum f\u00fcr Naturkunde, Leibniz-Institut f\u00fcr Evolutions- und Biodiversit\u00e4tsforschung , 10115 Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Ganzert","sequence":"additional","affiliation":[{"name":"Department of Plankton and Microbial Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries , 16775 Stechlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7986-5880","authenticated-orcid":false,"given":"Pedro","family":"Zamorano","sequence":"additional","affiliation":[{"name":"Laboratorio de Microorganismos Extrem\u00f3filos, University of Antofagasta , Antofagasta 02800 , Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Schulze-Makuch","sequence":"additional","affiliation":[{"name":"GFZ German Research Centre for Geosciences , Section Geomicrobiology, 14473 Potsdam , Germany"},{"name":"Zentrum f\u00fcr Astronomie und Astrophysik, Technische Universit\u00e4t Berlin , 10623 Berlin , Germany"},{"name":"Department of Plankton and Microbial Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries , 16775 Stechlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5064-497X","authenticated-orcid":false,"given":"Dirk","family":"Wagner","sequence":"additional","affiliation":[{"name":"GFZ German Research Centre for Geosciences , Section Geomicrobiology, 14473 Potsdam , Germany"},{"name":"Institute of Geosciences, University of Potsdam , 14476 Potsdam , 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