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While secretoneurin (SN) is a phylogenetically conserved secretogranin-2-derived peptide that stimulates Lh, its role in ovulation has not been established. To directly compare periovulatory changes in the classical hormones to the emerging reproductive neuropeptides SNa and SNb, simultaneous mass spectrometry measurement of 9 peptides and 5 steroids was conducted in female zebrafish. Regression analysis indicated that levels of SNa1\u201334 in the brain peaked when type 3 gonadotropin-releasing hormone (Gnrh3) increased (R2 = 0.71) at the time of the Lh surge, 3.5\u2005h before ovulation. Levels of the naturally occurring derivative SNa1\u201314 were highest at ovulation, while SNb1\u201331 was invariable. The bioactivities of SNa1\u201334 and SNa1\u201314 were investigated. After injection of SNa1\u201334 in females that had been isolated from males, 61% (11\/18) ovulated within 6\u2005h, which was like the effects of the Lh analog human chorionic gonadotropin (72%; 13\/18 females). SNa1\u201334 injection induces ovulation by increasing time-dependent expression of gnrh3 in the brain, a likely direct stimulation of chorionic gonadotropin alpha (cga) and luteinizing hormone b (lhb) subunit in pituitary, and via the subsequent time-dependent increase in nuclear progesterone receptor (npr) in ovaries. In contrast, SNa1\u201314 exhibited far fewer effects on gene expression and did not induce ovulation. Our results support the proposal that SN is a reproductive hormone.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgaf097","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T05:35:21Z","timestamp":1743744921000},"source":"Crossref","is-referenced-by-count":10,"title":["Hormonal dynamics reveal a stimulatory role for secretoneurin in zebrafish ovulation"],"prefix":"10.1093","volume":"4","author":[{"given":"Di","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa , Ottawa K1N 6N5, Ontario ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa , Ottawa K1N 6N5, Ontario ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5383-3149","authenticated-orcid":false,"given":"Victoria","family":"Spadacini","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa , Ottawa K1N 6N5, Ontario ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2029-2031","authenticated-orcid":false,"given":"Kimberly","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa , Ottawa K1N 6N5, Ontario ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Biology, Bioinformatics and Computational Biology Program, College of Arts and Sciences, Saint Louis University , Saint Louis, MO 63103-2010 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dapeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biology, Bioinformatics and Computational Biology Program, College of Arts and Sciences, Saint Louis University , Saint Louis, MO 63103-2010 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0183-9524","authenticated-orcid":false,"given":"Berta","family":"Levavi-Sivan","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Robert H. 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