{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:27:22Z","timestamp":1790191642949,"version":"4.1.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008630","name":"Children's Health Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008630","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001694","name":"International Retinal Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001694","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glaucoma is a major global cause of irreversible vision loss. It is marked by elevated intraocular pressure (IOP) and the loss of retinal ganglion cells (RGC). While there are medical and surgical therapies for glaucoma aiming to reduce aqueous humor production or enhance its drainage, these treatments are often inadequate for effectively managing the disease. In this study, we developed a targeted therapy for glaucoma by knocking down two genes associated with aqueous humor production (aquaporin 1 [AQP1] and carbonic anhydrase type 2 [CA2]) using Cas13 RNA editing systems. We demonstrate that hfCas13d-mediated knockdown of AQP1 and CA2 significantly lowers IOP in wild-type mice and in a corticosteroid-induced glaucoma mouse model. We show that the lowered IOP results from decreasing aqueous production without affecting the outflow facility; this treatment also significantly promotes RGC survival as compared with untreated control groups. Therefore, CRISPR\u2013Cas-based gene editing may be an effective treatment to lower IOP for glaucomatous optic neuropathy.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgaf168","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T08:05:06Z","timestamp":1750925106000},"source":"Crossref","is-referenced-by-count":10,"title":["Gene therapy for ocular hypertension using hfCas13d-mediated mRNA targeting"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9801-4917","authenticated-orcid":false,"given":"Siyu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5433-5656","authenticated-orcid":false,"given":"Zhiquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Hui","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8194-5680","authenticated-orcid":false,"given":"Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8994-3803","authenticated-orcid":false,"given":"Ke","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6300-6491","authenticated-orcid":false,"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0989-6593","authenticated-orcid":false,"given":"Jingyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Stanford University School of Medicine , 1651 Page Mill Road, Rm 2220, Palo Alto, CA 94304 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Medicine, John A. 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