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Elevated levels of VDGs are a hallmark of the autoantibodies most specific to rheumatoid arthritis and are also associated with other autoimmune diseases. Nonetheless, the effect of VDGs on IgG function remains poorly understood. This study investigates the impact of VDGs on the ability of antibodies to activate the complement system, a key immune effector mechanism. We demonstrate that VDGs on IgG inhibit the initiation of the classical complement pathway, as evidenced by complement activation assays with IgG displaying varying degrees of VDGs. Structure\u2013function analyses show that VDGs reduce classical complement activation by impeding IgG oligomer formation on antigenic surfaces. As IgG oligomerization is essential for effective C1q binding, VDG-mediated interference with this process lowers IgG's complement activation potential. This impaired ability to recruit complement was further substantiated through direct visualization of IgG oligomer\u2013C1q complexes on antigen-coated vesicles by cryoelectron tomography. Together, these data reveal that VDGs increase the functional diversity of IgG and identify a novel regulatory mechanism that modulates the ability of antibodies to activate one of their main immune effector mechanisms.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgaf216","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T18:02:40Z","timestamp":1752688960000},"source":"Crossref","is-referenced-by-count":1,"title":["Modulation of immunoglobulin G oligomerization by variable domain glycans: A mechanism to regulate complement activation"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3915-3472","authenticated-orcid":false,"given":"Roxane","family":"Biersteker","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7634-5353","authenticated-orcid":false,"given":"Leoni","family":"Abendstein","sequence":"additional","affiliation":[{"name":"Department of Cell and Chemical Biology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"van de Bovenkamp","sequence":"additional","affiliation":[{"name":"Department of Immunology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo J","family":"van Dooren","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8535-1728","authenticated-orcid":false,"given":"Sebastiaan M W R","family":"Hamers","sequence":"additional","affiliation":[{"name":"Department of Cell and Chemical Biology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanneke C","family":"Kwekkeboom","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9629-8950","authenticated-orcid":false,"given":"Eva Maria","family":"Stork","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan W","family":"Drijfhout","sequence":"additional","affiliation":[{"name":"Department of Immunology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9600-1312","authenticated-orcid":false,"given":"Theo","family":"Rispens","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam , 1066 CX Amsterdam ,","place":["The Netherlands"]},{"name":"Amsterdam Institute for Infection and Immunity , 1105 AZ, 1081 HV Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolien A M","family":"Koeleman","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-4995","authenticated-orcid":false,"given":"Manfred","family":"Wuhrer","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5186-2290","authenticated-orcid":false,"given":"Leendert A","family":"Trouw","sequence":"additional","affiliation":[{"name":"Department of Immunology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1990-2333","authenticated-orcid":false,"given":"Thomas H","family":"Sharp","sequence":"additional","affiliation":[{"name":"Department of Cell and Chemical Biology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]},{"name":"School of Biochemistry, University of Bristol , Bristol BS8 1TD ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-6414","authenticated-orcid":false,"given":"Ren\u00e9 E M","family":"Toes","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5749-8087","authenticated-orcid":false,"given":"Theresa","family":"Kissel","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center , 2333 ZA Leiden ,","place":["The 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