{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:30:03Z","timestamp":1772155803196,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["#1826135"],"award-info":[{"award-number":["#1826135"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["#2143997"],"award-info":[{"award-number":["#2143997"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institutes of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM150623"],"award-info":[{"award-number":["R35GM150623"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pemphigus vulgaris (PV) is a blistering autoimmune disease that affects the skin and mucous membranes. The mechanisms by which PV antibodies induce loss of cohesion in keratinocytes are not fully understood. It is accepted that the process starts with antibody binding to desmosomal targets, which leads to its disassembly and subsequent structural changes to cell\u2013cell adhesions. In vitro imaging of desmosome molecules has been used to characterize this initial phase. However, there remains an untapped potential of image analysis in providing us with more in-depth knowledge regarding biophysical changes after antibody binding. Currently, there is no quantitative framework from immunofluorescence images in PV pathology. Here, we seek to establish a correlation of biophysical changes with antibody pathogenicity by examining the effects of PV antibodies on adhesion molecules and the cytoskeletal network. Specifically, we introduced a data-driven approach to quantitatively evaluate perturbations in adhesion molecules following antibody treatment. We identify distinct imaging signatures that mark the impact of antibody binding on the remodeling of adhesion molecules and introduce a pathogenicity score to compare the relative effects of different antibodies. From this analysis, we showed that the biophysical response of keratinocytes to distinct PV antibodies is highly specific, allowing for accurate prediction of their pathogenicity. For instance, the high pathogenicity scores of the PVIgG and AK23 antibodies show strong agreement with their reported PV pathology. Our data-driven approach offers a detailed framework for the action of antibodies in pemphigus and paves the way for the development of effective diagnostic and therapeutic strategies.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgaf218","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T06:28:51Z","timestamp":1752820131000},"source":"Crossref","is-referenced-by-count":0,"title":["Data-driven image analysis to determine antibody-induced dissociation of cell\u2013cell adhesion and antibody pathogenicity in pemphigus"],"prefix":"10.1093","volume":"4","author":[{"given":"Amir","family":"Ostadi Moghaddam","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]},{"name":"Institute of Quantitative Health Science and Engineering, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]},{"name":"Department of Mechanical and Materials Engineering, University 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,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwei","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1916-0879","authenticated-orcid":false,"given":"Jordan","family":"Rosenbohm","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3309-2568","authenticated-orcid":false,"given":"Fanben","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4305-2938","authenticated-orcid":false,"given":"Animesh A","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University at Buffalo , Buffalo, NY 14203 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1361-4277","authenticated-orcid":false,"given":"Ruiguo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]},{"name":"Institute of Quantitative Health Science and Engineering, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]},{"name":"Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 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Anti-thyroid peroxidase antibodies target a cytoplasmic protein in keratinocytes and may contribute to blister formation in pemphigus vulgaris","volume":"139","author":"Seiffert-Sinha","year":"2019","journal-title":"J Invest Dermatol"},{"key":"2026020603513733800_pgaf218-B39","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.jid.2021.02.030","article-title":"014 a novel pemphigus vulgaris patient-derived antibody with sequence homology to antibodies directed against desmosomal and non-desmosomal targets induces keratinocyte dissociation","volume":"141","author":"Seiffert-Sinha","year":"2021","journal-title":"J Invest Dermatol"},{"key":"2026020603513733800_pgaf218-B40","doi-asserted-by":"crossref","first-page":"e50696","DOI":"10.1371\/journal.pone.0050696","article-title":"Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation","volume":"7","author":"Saito","year":"2012","journal-title":"PLoS 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